Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [8F7] (GTX70113) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded Breast ca, using BRCA1(GTX70113) antibody at 1:200 dilution.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [8F7] (GTX70113) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

The data was published in the journal PLoS One in 2013. PMID: 23805307

The data was published in the journal Breast Cancer Res in 2001. PMID: 11250747