Carbonic Anhydrase IX antibody detects Carbonic Anhydrase IX protein at cell membrane in human renal cell carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human renal cell carcinoma.
Carbonic Anhydrase IX antibody (GTX128428) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Carbonic Anhydrase IX antibody detects Carbonic Anhydrase IX protein at cell membrane by immunofluorescent analysis.
Sample: Mock and treated A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody (GTX128428) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Carbonic Anhydrase IX antibody (GTX128428) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Carbonic Anhydrase IX antibody detects Carbonic Anhydrase IX protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody (GTX128428) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Carbonic Anhydrase IX antibody detects Carbonic Anhydrase IX protein at membrane on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded cervical carcinoma.
Carbonic Anhydrase IX antibody (GTX128428) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Carbonic Anhydrase IX antibody (GTX128428) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Carbonic Anhydrase IX antibody (GTX128428) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Sci Rep in 2017.PMID: 28687750

The data was published in the journal Sci Rep in 2017.PMID: 28687750