Carbonic Anhydrase IX antibody

Cat. No. GTX128428

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, IHC
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all Carbonic Anhydrase IX products

View product citations for antibody GTX128428 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human Carbonic Anhydrase IX. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885777

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carbonic anhydrase 9 , CAIX , MN

Cellular Localization

Nucleus , nucleolus , Cell membrane , Cell projection , microvillus membrane

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq]

Database

Gene ID: 768 CA9
UniProt: Q16790 CA9

Research Area

- Cancer
- Cancer
- Hypoxia

DATA IMAGES

GTX128428 IHC-P Image

Carbonic Anhydrase IX antibody detects Carbonic Anhydrase IX protein at cell membrane in human renal cell carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human renal cell carcinoma.
Carbonic Anhydrase IX antibody (GTX128428) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX128428 ICC/IF Image

Carbonic Anhydrase IX antibody detects Carbonic Anhydrase IX protein at cell membrane by immunofluorescent analysis.
Sample: Mock and treated A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody (GTX128428) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX128428 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Carbonic Anhydrase IX antibody (GTX128428) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX128428 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Carbonic Anhydrase IX antibody (GTX128428) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX128428 IHC-P Image

Carbonic Anhydrase IX antibody detects Carbonic Anhydrase IX protein at membrane on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded cervical carcinoma.
Carbonic Anhydrase IX antibody (GTX128428) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX128428 IHC-P Image

Carbonic Anhydrase IX antibody detects Carbonic Anhydrase IX protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody (GTX128428) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min