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Estrogen Receptor alpha (phospho Ser118) antibody

Anti-Estrogen Receptor alpha (phospho Ser118) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50139
Anti-Estrogen Receptor alpha (phospho Ser118) antibody used in Western Blot (WB). GTX50139
Anti-Estrogen Receptor alpha (phospho Ser118) antibody used in Western Blot (WB). GTX50139
Anti-Estrogen Receptor alpha (phospho Ser118) antibody used in IHC (Paraffin sections) (IHC-P). GTX50139

Cat. No. GTX50139

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Monkey
Package
100 μl ($399)
See all Estrogen Receptor alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 118 (Q-L-S(p)-P-F) derived from human Estrogen Receptor alpha.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

estrogen receptor 1 , ER , ESR , ESRA , ESTRR , Era , NR3A1

Cellular Localization

Cytoplasm,Cell membrane,Cell membrane; Single-pass type I membrane protein,Golgi apparatus

Background

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-Estrogen Receptor alpha (phospho Ser118) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50139

GTX50139 ICC/IF Image

ICC/IF analysis of methanol-fixed MCF cells using GTX50139 Estrogen Receptor alpha (phospho Ser118) antibody.

Anti-Estrogen Receptor alpha (phospho Ser118) antibody used in Western Blot (WB). GTX50139

GTX50139 WB Image

WB analysis of extracts from COS-7 cells treated with starve or calf intestinal phosphatase (CIP) using GTX50139 Estrogen Receptor alpha (phospho Ser118) antibody.

Anti-Estrogen Receptor alpha (phospho Ser118) antibody used in Western Blot (WB). GTX50139

GTX50139 WB Image

WB analysis of extracts from MDA cells untreated or treated with Estradiol using GTX50139 Estrogen Receptor alpha (phospho Ser118) antibody.

Anti-Estrogen Receptor alpha (phospho Ser118) antibody used in IHC (Paraffin sections) (IHC-P). GTX50139

GTX50139 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50139 Estrogen Receptor alpha (phospho Ser118) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399