APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:20000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
Not tested in other applications.
Observed MW
36 kDa.
Positive Control
NIH-3T3 , mouse brain , PC-12 , rat brain , coli M15 , 293T , A431 , HeLa , HepG2
Predict Reactivity
Rabbit, Sheep, Bovine, Cat, Chicken, Guinea pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_11174761
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP
Cellular Localization
Cytoplasm , perinuclear region , Membrane
Background
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX627408 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627408 WB Image
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis. A. 20 μg 293T whole cell lysate/extract B. 10 μg 293T whole cell lysate/extract C. 5 μg 293T whole cell lysate/extract D. 1 μg 293T whole cell lysate/extract 10% SDS-PAGE GAPDH antibody [GT239] (GTX627408) dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627408 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627408 WB Image
Sample (30 μg of whole cell lysate) A: Drosophila 10% SDS PAGE GTX627408 diluted at 1:5000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627408 ICC/IF Image
GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: GAPDH stained by GAPDH antibody [GT239] (GTX627408) diluted at 1:500.Blue: Hoechst 33342 staining.
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GTX627408 WB Image
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627408 IHC-P Image
GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.GAPDH stained by GAPDH antibody [GT239] (GTX627408) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX627408 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
GTX627408 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
GTX627408 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
GTX627408 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627408 WB Image
Yeast lysate was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627408 WB Image
The data was published in the Nat Commun in 2020. PMID: 33257668
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GTX627408 WB Image
The data was published in the journal Oncotarget in 2015.PMID: 26327325
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GTX627408 WB Image
The data was published in the journal Front Physiol in 2019. PMID: 31681015
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GTX627408 WB Image
The data was published in the journal Biomed Res Int in 2015. PMID: 26448933
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GTX627408 WB Image
The data was published in the journal Biomed Res Int in 2015. PMID: 26448933
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GTX627408 WB Image
The data was published in the journal PLoS One in 2016. PMID: 27832139
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GTX627408 WB Image
The data was published in the journal J Biol Chem in 2019.PMID: 30898877
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GTX627408 WB Image
The data was published in the journal J Biol Chem in 2016.PMID: 26742848
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2015.PMID: 26681405
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GTX627408 WB Image
The data was published in the journal PLoS One in 2014.PMID: 24718614
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GTX627408 WB Image
The data was published in the journal J Biol Chem in 2016.PMID: 26742848
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2016.PMID: 27681590
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2018.PMID: 29933620
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2016.PMID: 27307030
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GTX627408 WB Image
The data was published in the 2022 in Int J Mol Sci. PMID: 35054778
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GTX627408 WB Image
The data was published in the 2020 in Cell Commun Signal. PMID: 33228717
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GTX627408 WB Image
The data was published in the 2020 in PLoS One. PMID: 32163428
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GTX627408 WB Image
The data was published in the 2022 in Nat Commun. PMID: 35618735
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GTX627408 WB Image
The data was published in the 2016 in PLoS Genet. PMID: 27727273
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GTX627408 WB Image
The data was published in the 2022 in Nat Commun. PMID: 35618735
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GTX627408 WB Image
The data was published in the 2022 in Nat Commun. PMID: 35618735
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GTX627408 WB Image
The data was published in the 2022 in EMBO Mol Med. PMID: 35253392
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GTX627408 WB Image
The data was published in the 2022 in Front Aging Neurosci. PMID: 35264943
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GTX627408 WB Image
The data was published in the 2022 in Front Aging Neurosci. PMID: 35264943
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GTX627408 WB Image
The data was published in the 2016 in PLoS Genet. PMID: 27727273
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GTX627408 WB Image
The data was published in the 2021 in Nat Commun. PMID: 33712616
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2015.PMID: 26681405
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2017.PMID: 29038428
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GTX627408 WB Image
The data was published in the journal Nat Commun in 2016.PMID: 27492621
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2015.PMID: 26681405
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GTX627408 WB Image
The data was published in the journal J Cancer Sci Ther in 2018.PMID: 29780443
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2018.PMID: 29933620
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2016.PMID: 27681590
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GTX627408 WB Image
The data was published in the journal Oxid Med Cell Longev in 2020.PMID: 32089770
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GTX627408 WB Image
The data was published in the journal PLoS One in 2013.PMID: 23977396
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GTX627408 WB Image
The data was published in the journal Front Neurol in 2019.PMID: 31258504
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GTX627408 WB Image
The data was published in the journal Oncotarget in 2018.PMID: 29581854
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GTX627408 WB Image
The data was published in the journal ASN Neuro in 2013.PMID: 24286475
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GTX627408 WB Image
The data was published in the 2022 in Sci Rep. PMID: 35484165
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GTX627408 WB Image
The data was published in the 2022 in Pathogens. PMID: 35335638
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GTX627408 WB Image
The data was published in the J Cell Mol Med in 2020. PMID: 32827244
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GTX627408 WB Image
The data was published in the J Cell Mol Med in 2020. PMID: 32827244
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GTX627408 WB Image
The data was published in the J Cell Mol Med in 2020. PMID: 32827244
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GTX627408 WB Image
The data was published in the J Cell Mol Med in 2020. PMID: 32827244
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GTX627408 WB Image
The data was published in the journal J Biol Chem in 2016.PMID: 26742848
|
|
GTX627408 WB Image
The data was published in the journal Sci Rep in 2015.PMID: 26681405
|
|
GTX627408 WB Image
The data was published in the journal EMBO Mol Med in 2013.PMID: 23828861
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|
GTX627408 WB Image
The data was published in the journal Sci Rep in 2016.PMID: 27681590
|
|
GTX627408 WB Image
The data was published in the journal J Biol Chem in 2016.PMID: 26742848
|
|
GTX627408 WB Image
The data was published in the journal J Biol Chem in 2016.PMID: 26742848
|
|
GTX627408 WB Image
The data was published in the journal J Biol Chem in 2016.PMID: 26742848
|
|
GTX627408 WB Image
The data was published in the journal Front Neurol in 2019.PMID: 31258504
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GTX627408 WB Image
The data was published in the journal PLoS One in 2013.PMID: 24282540
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GTX627408 WB Image
The data was published in the journal J Cell Mol Med in 2015.PMID: 25683605
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GTX627408 WB Image
The data was published in the journal Nat Commun in 2019.PMID: 31337756
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2020.PMID: 32150830
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GTX627408 WB Image
The data was published in the journal Cell Death Dis in 2020.PMID: 32034133
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GTX627408 WB Image
The data was published in the journal Nat Commun in 2017.PMID: 28198375
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2018.PMID: 29933620
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GTX627408 WB Image
The data was published in the journal Nat Commun in 2018.PMID: 30030436
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GTX627408 WB Image
The data was published in the journal J Cancer Sci Ther in 2018.PMID: 29780443
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2017.PMID: 28747643
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GTX627408 WB Image
The data was published in the journal Front Cell Neurosci in 2014. PMID: 25071443
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GTX627408 WB Image
The data was published in the journal Front Cell Neurosci in 2014. PMID: 25071443
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GTX627408 WB Image
The data was published in the journal Acta Neuropathol in 2018. PMID: 28780615
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GTX627408 WB Image
The data was published in the journal Acta Neuropathol in 2018. PMID: 28780615
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GTX627408 WB Image
The data was published in the journal Nat Commun in 2016. PMID: 27460411
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GTX627408 WB Image
The data was published in the journal Acta Neuropathol in 2018. PMID: 28780615
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GTX627408 WB Image
The data was published in the journal PLoS One in 2018. PMID: 29444136
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2014. PMID: 24406502
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GTX627408 WB Image
The data was published in the journal Mol Oncol in 2017. PMID: 28100038
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2017. PMID: 28829370
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GTX627408 WB Image
The data was published in the 2022 in Sci Rep. PMID: 35484165
|
|
GTX627408 WB Image
The data was published in the 2022 in Pathogens. PMID: 35335638
|
|
GTX627408 WB Image
The data was published in the 2020 in Cell Commun Signal. PMID: 33228717
|
|
GTX627408 WB Image
The data was published in the 2020 in Cell Commun Signal. PMID: 33228717
|
|
GTX627408 WB Image
The data was published in the 2020 in Cell Commun Signal. PMID: 33228717
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GTX627408 WB Image
The data was published in the 2020 in Cell Commun Signal. PMID: 33228717
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GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799
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GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31311187
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GTX627408 WB Image
The data was published in the journal Cell Death Dis in 2017. PMID: 29238067
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GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31311187
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GTX627408 WB Image
The data was published in the journal Cells in 2019. PMID: 31766533
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GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31311187
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GTX627408 WB Image
The data was published in the journal PLoS One in 2015. PMID: 26110811
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GTX627408 WB Image
The data was published in the journal Oncogene in 2017. PMID: 28319065
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GTX627408 WB Image
The data was published in the journal Hum Mol Genet in 2015. PMID: 26085575
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GTX627408 WB Image
The data was published in the journal Cell Death Dis in 2017. PMID: 29238067
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GTX627408 WB Image
The data was published in the journal Oncogene in 2017. PMID: 28319065
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GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2020. PMID: 31947604
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GTX627408 WB Image
The data was published in the journal Cell Death Dis in 2017. PMID: 29238067
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GTX627408 WB Image
The data was published in the journal Cells in 2019. PMID: 31766533
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GTX627408 WB Image
The data was published in the journal Cardiovasc Diabetol in 2018. PMID: 29903013
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GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2019. PMID: 31323961
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2019. PMID: 31323961
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GTX627408 WB Image
The data was published in the journal Cells in 2019. PMID: 31640218
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GTX627408 WB Image
The data was published in the journal Cells in 2019. PMID: 31766533
|
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GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799
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GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31311187
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GTX627408 WB Image
The data was published in the journal Molecules in 2017. PMID: 28531143
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GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799
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GTX627408 WB Image
The data was published in the journal Oncotarget in 2017. PMID: 27926533
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GTX627408 WB Image
The data was published in the journal Oncogene in 2017. PMID: 28319065
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GTX627408 WB Image
The data was published in the journal PLoS One in 2014. PMID: 25166596
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GTX627408 WB Image
The data was published in the journal Nucleic Acids Res in 2013. PMID: 23658231
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2017. PMID: 28829370
|
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GTX627408 WB Image
The data was published in the journal PLoS One in 2018. PMID: 29444136
|
|
GTX627408 WB Image
The data was published in the journal Sci Rep in 2017. PMID: 28157211
|
|
GTX627408 WB Image
The data was published in the journal Acta Neuropathol Commun in 2019. PMID: 30626447
|
|
GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2015. PMID: 26151845
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|
GTX627408 WB Image
The data was published in the journal Acta Neuropathol Commun in 2019. PMID: 30626447
|
|
GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2015. PMID: 26151845
|
|
GTX627408 WB Image
The data was published in the journal Acta Neuropathol Commun in 2019. PMID: 30626447
|
|
GTX627408 WB Image
The data was published in the journal Acta Neuropathol Commun in 2019. PMID: 30626447
|
|
GTX627408 WB Image
The data was published in the journal Cardiovasc Diabetol in 2018. PMID: 29903013
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|
GTX627408 WB Image
The data was published in the journal Front Cell Neurosci in 2014. PMID: 25071443
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GTX627408 WB Image
The data was published in the journal J Virol in 2017. PMID: 28679762
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GTX627408 WB Image
The data was published in the journal J Virol in 2017. PMID: 28679762
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GTX627408 WB Image
The data was published in the journal Biomed Res Int in 2014. PMID: 25162007
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GTX627408 WB Image
The data was published in the journal Commun Biol in 2019. PMID: 31754649
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GTX627408 WB Image
The data was published in the journal Biomed Res Int in 2014. PMID: 25162007
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GTX627408 WB Image
The data was published in the journal Biomed Res Int in 2014. PMID: 25162007
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GTX627408 WB Image
The data was published in the journal Biomed Res Int in 2014. PMID: 25162007
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GTX627408 WB Image
The data was published in the journal Nat Commun in 2017. PMID: 28134239
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GTX627408 WB Image
The data was published in the journal Nat Commun in 2017. PMID: 28134239
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GTX627408 WB Image
The data was published in the journal eNeuro in 2017. PMID: 28856243
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GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799
|
|
GTX627408 WB Image
The data was published in the journal PLoS One in 2014. PMID: 25166596
|
|
GTX627408 WB Image
The data was published in the journal PLoS One in 2014. PMID: 25166596
|
|
GTX627408 WB Image
The data was published in the journal PLoS One in 2014. PMID: 25166596
|
|
GTX627408 WB Image
The data was published in the journal Cell Death Dis in 2017. PMID: 29238067
|
|
GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799
|
|
GTX627408 WB Image
The data was published in the journal Oncogene in 2017. PMID: 28319065
|
|
GTX627408 WB Image
The data was published in the journal Sci Rep in 2017. PMID: 29273797
|
|
GTX627408 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31311187
|
|
GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2020.PMID: 32150830
|
|
GTX627408 WB Image
The data was published in the journal Sci Rep in 2015.PMID: 26681405
|
|
GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2020.PMID: 32150830
|
|
GTX627408 WB Image
The data was published in the journal Cell Death Dis in 2020.PMID: 32034133
|
|
GTX627408 WB Image
The data was published in the journal Oncotarget in 2016.PMID: 27708250
|
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2018.PMID: 29933620
|
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GTX627408 WB Image
The data was published in the journal Int J Mol Sci in 2018.PMID: 29933620
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GTX627408 WB Image
The data was published in the journal Curr Biol in 2017.PMID: 28966089
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GTX627408 WB Image
The data was published in the journal Sci Rep in 2016.PMID: 27222304
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GTX627408 WB Image
The data was published in the journal J Biol Chem in 2016.PMID: 26742848
|
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GTX627408 WB Image
The data was published in the journal Front Neurol in 2019.PMID: 31258504
|
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GTX627408 WB Image
The data was published in the journal J Biol Chem in 2016.PMID: 26742848
|
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GTX627408 WB Image
The data was published in the journal Oxid Med Cell Longev in 2020.PMID: 32089770
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REFERENCE
REVIEW
GAPDH antibody [GT239]
Cat. No. GTX627408
Rating
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( Average 4.5 based on 6 users reviews)
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Application
Western Blot(WB)
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( Average 4.5 based on 6 users reviews)
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Date :
Anonymous submitted on 24-Apr-2017
Application Tested :
WB
Sample Species :
Rat
Sample :
Brain tissue
Amount used :
50 μg
Blocking :
3% BSA in TBST, 23°C, 1Hr
Primary Antibody :
1:10000, 4°C, 16Hr
Date :
Anonymous submitted on 30-Jun-2014
Application Tested :
WB
Sample Species :
Hu
Sample :
KATOIII cells, MKN45 cells
Amount used :
100μg
Blocking :
skim milk, 25°C, 1Hr
Primary Antibody :
1:5000, 4°C, 16Hr
Lane Description :
Lane1:KATOIII cells, Lane2:MKN45 cells
Date :
Anonymous submitted on 18-Apr-2014
Application Tested :
WB
Sample Species :
Hu
Sample :
SHSY-5Y cells
Amount used :
30μg
Blocking :
5% Skim milk in TBST, 25°C, 2Hr
Primary Antibody :
1:5000, 4°C, 16Hr
Date :
Anonymous submitted on 06-Mar-2014
Application Tested :
WB
Sample Species :
Rat
Sample :
PC12 cells
Amount used :
20μg
Blocking :
5% nonfat milk, 22°C, 0.5Hr
Primary Antibody :
1:1000, 22°C, 2Hr
Figure Note :
starreivew courtesy of Xiaobei(Laboratory of UCSD)
Date :
Anonymous submitted on 06-Feb-2012
Application Tested :
WB
Sample Species :
Hu
Sample :
293T cells
Amount used :
30μg
Blocking :
TBST with 5% milk, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 06-Feb-2012
Application Tested :
WB
Sample Species :
Hu
Sample :
M10 cells
Amount used :
40μg
Blocking :
TBST with 5% milk, 25°C, 1Hr
Primary Antibody :
1:5000, 25°C, 1Hr