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HIF1 alpha antibody [GT10211]

Orthogonal
Anti-HIF1 alpha antibody [GT10211] used in Western Blot (WB). GTX628480
Anti-HIF1 alpha antibody [GT10211] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628480
Anti-HIF1 alpha antibody [GT10211] used in Western Blot (WB). GTX628480
Anti-HIF1 alpha antibody [GT10211] used in Immunoprecipitation (IP). GTX628480
Anti-HIF1 alpha antibody [GT10211] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628480
Anti-HIF1 alpha antibody [GT10211] used in Western Blot (WB). GTX628480
Anti-HIF1 alpha antibody [GT10211] used in Western Blot (WB). GTX628480
Anti-HIF1 alpha antibody [GT10211] used in Western Blot (WB). GTX628480

Cat. No. GTX628480

Reference 15
3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT10211

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IP, IHC-P (cell pellet)

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all HIF1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP 1:100-1:500
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

HepG2 (1%O2 treatment for 48hr)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human HIF1 alpha

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888039

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hypoxia inducible factor 1 subunit alpha , HIF-1-alpha , HIF-1A , HIF-1alpha , HIF1 , HIF1-ALPHA , MOP1 , PASD8 , bHLHe78

Cellular Localization

Cytoplasm , Nucleus

Background

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HIF1 alpha antibody [GT10211] used in Western Blot (WB). GTX628480

GTX628480 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody [GT10211] (GTX628480) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody [GT10211] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628480

GTX628480 ICC/IF Image

Orthogonal

HIF1 alpha antibody [GT10211] detects HIF1 alpha protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF1 alpha stained by HIF1 alpha antibody [GT10211] (GTX628480) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-HIF1 alpha antibody [GT10211] used in Western Blot (WB). GTX628480

GTX628480 WB Image

Orthogonal

HIF1 alpha antibody [GT10211] detects HIF1A protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract (untreated)
B. 30 μg HepG2 whole cell lysate/extract (1%O2 treatment for 48hr)
7.5% SDS-PAGE
HIF1 alpha antibody [GT10211] (GTX628480) dilution: 1:250
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody [GT10211] used in Immunoprecipitation (IP). GTX628480

GTX628480 IP Image

HIF-1 alpha antibody immunoprecipitates HIF-1 alpha protein in IP experiments.
IP Sample: 1000 μg HepG2 whole cell lysate/extract
A. 40 μg HepG2 whole cell lysate/extract from normoxia
B. 40 μg HepG2 whole cell lysate/extract from hypoxia 24hr (1%O2)
C. Control with 2.5 μg of preimmune mouse IgG/HepG2 whole cell
lysate/extract from normoxia
D. Control with 2.5 μg of preimmune mouse IgG/ HepG2 whole cell
lysate/extract from hypoxia 24hr (1%O2)
E. Immunoprecipitation of HIF-1 alpha protein by 2.5 μg of HIF-1
alpha antibody (GTX628480)/HepG2 whole cell lysate/extract
from normoxia
F. Immunoprecipitation of HIF-1 alpha protein by 2.5 μg of HIF-1
alpha antibody (GTX628480)/HepG2 whole cell lysate/extract
from hypoxia 24hr (1%O2)
10% SDS-PAGE
The immunoprecipitated HIF-1 alpha protein was detected by HIF-1 alpha antibody (GTX628480) diluted at 1:500.
EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
Anti-HIF1 alpha antibody [GT10211] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628480

GTX628480 ICC/IF Image

HIF1 alpha antibody detects HIF1 alpha protein at nuclear by confocal immunofluorescent analysis. Sample: Hypoxia (500μM CoCl2) treated 24hr (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Red: HIF1 alpha protein stained by HIF1 alpha antibody (GTX628480) diluted at 1:250. Green: Alpha-tubulin, a cytoskeleton marker, stained by Rabbit Polyclonal antibody (GTX102078) diluted at 1:500. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

Anti-HIF1 alpha antibody [GT10211] used in Western Blot (WB). GTX628480

GTX628480 WB Image

Orthogonal

HIF1 alpha antibody [GT10211] detects HIF1 alpha protein by Western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract (untreated)
B. 30 μg HepG2 whole cell lysate/extract (500 μM CoCl2 treatment for 24 hr)
7.5 % SDS-PAGE
HIF1 alpha antibody [GT10211] (GTX628480) dilution: 1:500

Anti-HIF1 alpha antibody [GT10211] used in Western Blot (WB). GTX628480

GTX628480 WB Image

Orthogonal

The data was published in the journal Nat Commun in 2016.PMID: 27094744

Anti-HIF1 alpha antibody [GT10211] used in Western Blot (WB). GTX628480

GTX628480 WB Image

Orthogonal

The data was published in the journal Genome Biol in 2019.PMID: 30791937

REFERENCE

Application Reference

DR Sojka et al. Biochim Biophys Acta Mol Cell Res 2024; 1871 (5) : 119735 The human testis-enriched HSPA2 interacts with HIF-1α in epidermal keratinocytes, yet HIF-1α stability and HIF-1-dependent gene expression rely on the HSPA (HSP70) activity
Application : WB, IHC-P (cell pellet)
Keitoku M et al. J Clin Med 2022; 11 (24) Differential Recovery Patterns of the Maxilla and Mandible after Eliminating Nasal Obstruction in Growing Rats.
Application : IHC-P
Reactivity : Rat
Yao-Lung Chang et al. Genes (Basel) 2022; 13 (5) Effect of Hypoxia on Glucose Transporter 1 and 3 Gene Expression in Placental Mesenchymal Stem Cells Derived from Growth-Restricted Fetuses.
Application : WB
Reactivity : Human
Chen Zhang et al. Int J Med Sci 2021; 18 (15) : 3403-3411 Cobalt chloride-stimulated hypoxia promotes the proliferation of cholesteatoma keratinocytes via the PI3K/Akt signaling pathway.
Application : WB, IHC-P
Reactivity : Human
Beak JY et al. J Biol Chem 2021; 297 (6) : 101358 The nuclear receptor RORα preserves cardiomyocyte mitochondrial function by regulating caveolin-3-mediated mitophagy.
Application : WB
Reactivity : Rat
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Submit a Reference

REVIEW

HIF1 alpha antibody [GT10211] Cat. No. GTX628480

Rating
( Average 5 based on 3 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-HIF1 alpha antibody [GT10211] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628480
Date : Anonymous submitted on 29-Jan-2016
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : A549 cells
Fixation : 4% PFA, 2Hr
Permeabilization /Antigen retrieval : Citrate buffer, 95°C, 20minHr
Primary Antibody : 1:300, 25°C, 0.5Hr
Figure Note : A: A549 cells, B: A549 cells (1mM DMOG treatment for 3.5 h)
Anti-HIF1 alpha antibody [GT10211] used in IHC (Paraffin sections) (IHC-P). GTX628480
Date : Anonymous submitted on 29-Jan-2016
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : brain tissue
Fixation : 4% PFA, 2Hr
Permeabilization /Antigen retrieval : Citrate buffer, 95°C, 20minHr
Primary Antibody : 1:300, 25°C, 0.5Hr
Figure Note : A:Human glioblastoma tissue, B:Human healthy brain tissue
Anti-HIF1 alpha antibody [GT10211] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628480
Date : Anonymous submitted on 19-Oct-2012
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HeLa cells
Fixation : 4% paraformaldehyde
Primary Antibody : 1:250, 4°C, 16Hr
Figure Note : Right : Hypoxia (500uM CoCl2) treated 24hr, Left : untreated HeLa cells, Red: GTX628480, Green: Alpha-tubulin
Submit a Review
Application Reference
DR Sojka et al. Biochim Biophys Acta Mol Cell Res 2024; 1871 (5):119735 The human testis-enriched HSPA2 interacts with HIF-1α in epidermal keratinocytes, yet HIF-1α stability and HIF-1-dependent gene expression rely on the HSPA (HSP70) activity
Application : WB, IHC-P (cell pellet)
Keitoku M et al. J Clin Med 2022; 11 (24) Differential Recovery Patterns of the Maxilla and Mandible after Eliminating Nasal Obstruction in Growing Rats.
Application : IHC-P
Reactivity : Rat
Yao-Lung Chang et al. Genes (Basel) 2022; 13 (5) Effect of Hypoxia on Glucose Transporter 1 and 3 Gene Expression in Placental Mesenchymal Stem Cells Derived from Growth-Restricted Fetuses.
Application : WB
Reactivity : Human
Chen Zhang et al. Int J Med Sci 2021; 18 (15):3403-3411 Cobalt chloride-stimulated hypoxia promotes the proliferation of cholesteatoma keratinocytes via the PI3K/Akt signaling pathway.
Application : WB, IHC-P
Reactivity : Human
Beak JY et al. J Biol Chem 2021; 297 (6):101358 The nuclear receptor RORα preserves cardiomyocyte mitochondrial function by regulating caveolin-3-mediated mitophagy.
Application : WB
Reactivity : Rat
Sharma S et al. Genome Biol 2019; 20 (1):37 Mitochondrial hypoxic stress induces widespread RNA editing by APOBEC3G in natural killer cells.
Application : WB
Reactivity : Human
Masola V et al. Oncotarget 2018; 9 (90):36185-36201 Inhibition of heparanase protects against chronic kidney dysfunction following ischemia/reperfusion injury.
Application : WB
Matsumura-Matsuda E et al. Intern Med 2018; 57 (7):979-985 A Rare Coexistence of Pheochromocytoma and Parkinson's Disease With Diagnostic Challenges.
Application : IHC-P
Reactivity : Human
Sharma S et al. Hum Mol Genet 2017; 26 (7):1328-1339 Mitochondrial complex II regulates a distinct oxygen sensing mechanism in monocytes.
Saki M et al. Mol Cancer Res 2017; 15 (11):1503-1516 EGFR Mutations Compromise Hypoxia-Associated Radiation Resistance through Impaired Replication Fork-Associated DNA Damage Repair.
Shih JW et al. Nat Commun 2017; 8:15874 Long noncoding RNA LncHIFCAR/MIR31HG is a HIF-1α co-activator driving oral cancer progression.
Application : WB
Reactivity : Human
Berezowska S et al. Virchows Arch 2017; (Epub) Glycine decarboxylase and HIF-1α expression are negative prognostic factors in primary resected early-stage non-small cell lung cancer.
Application : IHC-P
Reactivity : Human
Bourseau-Guilmain E et al. Nat Commun 2016; 7:11371 Hypoxia regulates global membrane protein endocytosis through caveolin-1 in cancer cells.
Application : WB
Reactivity : Human
Biao Ma et al. Nat Cell Biol. 2014; 17 (1):95-103 Hypoxia regulates Hippo signalling through the SIAH2 ubiquitin E3 ligase.
Application : ICC/IF
Reactivity : Human
Cho SY et al. Evid Based Complement Alternat Med 2013; 2013:974313 Activation of AMP-Activated Protein Kinase α and Extracelluar Signal-Regulated Kinase Mediates CB-PIC-Induced Apoptosis in Hypoxic SW620 Colorectal Cancer Cells.
Application : WB
Reactivity : Human
SDS
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Package List Price ($)
$ 399
$ 169