GeneTex

  • Country / Location Selection

United States (US)

HIF1AN antibody

Anti-HIF1AN antibody used in Western Blot (WB). GTX116251
Anti-HIF1AN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116251
Anti-HIF1AN antibody used in IHC (Paraffin sections) (IHC-P). GTX116251
Anti-HIF1AN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116251

Cat. No. GTX116251

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HIF1AN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

HCT116 , MCF-7

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HIF1AN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176323

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hypoxia inducible factor 1 subunit alpha inhibitor , FIH1

Cellular Localization

Nucleus

Background

Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.

Database

Research Area

DATA IMAGES

Anti-HIF1AN antibody used in Western Blot (WB). GTX116251

GTX116251 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
B: MCF-7
10% SDS PAGE
GTX116251 diluted at 1:1000

Anti-HIF1AN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116251

GTX116251 ICC/IF Image

HIF1AN antibody detects HIF1AN protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF1AN protein stained by HIF1AN antibody (GTX116251) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-HIF1AN antibody used in IHC (Paraffin sections) (IHC-P). GTX116251

GTX116251 IHC-P Image

HIF1AN antibody detects HIF1AN protein at cytosol on U373 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U373 xenograft.
HIF1AN antibody (GTX116251) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HIF1AN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116251

GTX116251 ICC/IF Image

HIF1AN antibody detects HIF1AN protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF1AN protein stained by HIF1AN antibody (GTX116251) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

REFERENCE

There are currently no references for HIF1AN antibody (GTX116251). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for HIF1AN antibody (GTX116251). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169