HIF2 alpha antibody [N1N3]

Name: HIF2 alpha antibody [N1N3]
Brand: GeneTex
SKU: GTX103707
Price: 399.00 USD
Rating: 4 (2 reviews)

Anti-HIF2 alpha antibody [N1N3] used in Western Blot (WB). GTX103707

Anti-HIF2 alpha antibody [N1N3] used in ChIP assay (ChIP assay). GTX103707

Cat. No. GTX103707

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ChIP assay
Reactivity	Human, Zebrafish
Package	100 μl ($399), 25 μl ($169)

See all HIF2 alpha products

View product citations for antibody GTX103707 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ChIP assay	Assay dependent

Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Positive Control

HepG2 (1% O2 treatment for 24hr)

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HIF2 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617613

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

endothelial PAS domain protein 1 , ECYT4 , HIF2A , HLF , MOP2 , PASD2 , bHLHe73

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq]

Database

Gene ID: 2034 EPAS1
UniProt: Q99814 EPAS1

Research Area

- Cancer
- Cancer
- Hypoxia

DATA IMAGES

GTX103707 WB Image

HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract
B. 30 μg HepG2 whole cell lysate/extract (1% O2 treatment for 24 hr)
7.5% SDS-PAGE
HIF2 alpha antibody (GTX103707) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103707 ChIP assay Image

The data was published in the journal PLoS One in 2014. PMID: 25000307

GTX103707 WB Image

The data was published in the journal Oncol Lett in 2015. PMID: 26622817

GTX103707 WB Image

The data was published in the journal Oncol Lett in 2015. PMID: 26622817

REFERENCE

Application Reference

Abd-Aziz N et al. Oncol Lett 2015; 10 (4) : 2192-2196 * attenuates hif-1- but not hif-2-mediated transcriptional activation.

Lin TY et al. PLoS One 2014; 9 (7) : e101980 Hypoxia-Inducible Factor 2 Alpha Is Essential for Hepatic Outgrowth and Functions via the Regulation of leg1 Transcription in the Zebrafish Embryo.

Application : ChIP assay

Reactivity : Zebrafish

Ko CY et al. Cell Death Differ 2011; 18 (11) : 1757-70 Integration of CNS survival and differentiation by HIF2α.

Application : ChIP assay

Reactivity : Zebrafish

Submit a Reference

REVIEW