Immunofluorescence analysis of methanol-fixed HCT116, using HSP90 beta(GTX101423) antibody at 1:500 dilution.

HSP90 beta antibody [N2C2], Internal detects HSP90AB1 protein at cytosol on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HSP90 beta antibody [N2C2], Internal (GTX101423) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX101423 diluted at 1:2000

HSP90 beta antibody [N2C2], Internal detects HSP90 beta protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
HSP90 beta antibody [N2C2], Internal (GTX101423) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody [N2C2], Internal (GTX101423) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody [N2C2], Internal (GTX101423) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 beta antibody [N2C2], Internal (GTX101423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A: zebrafish eye
7.5% SDS PAGE
GTX101423 diluted at 1:1000

Hsp90 beta antibody [N2C2], Internal detects Hsp90 beta protein at cytosol on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Hsp90 beta antibody [N2C2], Internal (GTX101423) dilution: 1:200.

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Hsp90 beta antibody [N2C2], Internal (GTX101423) at 1:300 dilution.