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Hsp90 beta antibody [N2C2], Internal

Anti-Hsp90 beta antibody [N2C2], Internal used in Western Blot (WB). GTX101423
Anti-Hsp90 beta antibody [N2C2], Internal used in Western Blot (WB). GTX101423
Anti-Hsp90 beta antibody [N2C2], Internal used in Western Blot (WB). GTX101423
Anti-Hsp90 beta antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101423
Anti-Hsp90 beta antibody [N2C2], Internal used in Western Blot (WB). GTX101423
Anti-Hsp90 beta antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101423
Anti-Hsp90 beta antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101423
Anti-Hsp90 beta antibody [N2C2], Internal used in IHC (Whole mount) (IHC-Wm). GTX101423
Anti-Hsp90 beta antibody [N2C2], Internal used in Western Blot (WB). GTX101423
Anti-Hsp90 beta antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101423

Cat. No. GTX101423

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all Hsp90 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

HeLaS3 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

5.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSP90 beta. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1240970

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein 90 alpha family class B member 1 , D6S182 , HSP84 , HSP90B , HSPC2 , HSPCB

Cellular Localization

Cytoplasm , Melanosome

Background

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1 (MIM 140571), an inducible form, and HSP90AB1, a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Hsp90 beta antibody [N2C2], Internal used in Western Blot (WB). GTX101423

GTX101423 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 beta antibody [N2C2], Internal (GTX101423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Drosophila Hsp83 is the homolog of mammalian Hsp90. (Gene ID: 38389/ UniProt: P02828)

Anti-Hsp90 beta antibody [N2C2], Internal used in Western Blot (WB). GTX101423

GTX101423 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody [N2C2], Internal (GTX101423) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hsp90 beta antibody [N2C2], Internal used in Western Blot (WB). GTX101423

GTX101423 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody [N2C2], Internal (GTX101423) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hsp90 beta antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101423

GTX101423 IHC-P Image

HSP90 beta antibody [N2C2], Internal detects HSP90 beta protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
HSP90 beta antibody [N2C2], Internal (GTX101423) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Hsp90 beta antibody [N2C2], Internal used in Western Blot (WB). GTX101423

GTX101423 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX101423 diluted at 1:2000

Anti-Hsp90 beta antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101423

GTX101423 IHC-P Image

HSP90 beta antibody [N2C2], Internal detects HSP90AB1 protein at cytosol on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HSP90 beta antibody [N2C2], Internal (GTX101423) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Hsp90 beta antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101423

GTX101423 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HCT116, using HSP90 beta(GTX101423) antibody at 1:500 dilution.

Anti-Hsp90 beta antibody [N2C2], Internal used in IHC (Whole mount) (IHC-Wm). GTX101423

GTX101423 IHC-Wm Image

Hsp90 beta antibody [N2C2], Internal detects Hsp90 beta protein at cytosol on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Hsp90 beta antibody [N2C2], Internal (GTX101423) dilution: 1:200.
Anti-Hsp90 beta antibody [N2C2], Internal used in Western Blot (WB). GTX101423

GTX101423 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
7.5% SDS PAGE
GTX101423 diluted at 1:1000

Anti-Hsp90 beta antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101423

GTX101423 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Hsp90 beta antibody [N2C2], Internal (GTX101423) at 1:300 dilution.

REFERENCE

Application Reference

Galmozzi A et al. Nature 2019; 576 (7785) : 138-142 PGRMC2 is an intracellular haem chaperone critical for adipocyte function.
Application : WB
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169