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Influenza A virus M2 (matrix protein) antibody

Anti-Influenza A virus M2 (matrix protein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125951
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

Cat. No. GTX125951

Reference 34
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, EM

Reactivity

 Influenza A virus
Package
100 μl ($399),
25 μl ($169)
See all Influenza A virus M2 (matrix protein) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
EM Assay dependent
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

WSN-infected DF-1 cell

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein corresponding to Influenza A virus M2 (matrix protein) (A/Puerto Rico/8/1934(H1N1). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170983

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza A Virus Membrane 2 protein , Influenza A Virus M2

Research Area

DATA IMAGES

Anti-Influenza A virus M2 (matrix protein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125951

GTX125951 ICC/IF Image

Influenza A Virus M2 antibody detects M2 protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: M2 protein stained by Influenza A Virus M2 antibody (GTX125951) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

GTX125951 WB Image

Sample (50 μg of whole cell lysate)
A: mock DF-1 cell lysates
B: WSN-infected DF-1 cell lysates
15% SDS PAGE
GTX125951 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

GTX125951 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Influenza A virus M2 (matrix protein) antibody (GTX125951) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

GTX125951 WB Image

The data was published in the journal J Virol in 2017.PMID: 27795429

Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

GTX125951 WB Image

The data was published in the journal PLoS Pathog in 2018.PMID: 29723291

Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

GTX125951 WB Image

The data was published in the journal J Virol in 2017.PMID: 27795429

Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

GTX125951 WB Image

The data was published in the journal Front Microbiol in 2017. PMID: 29312159

REFERENCE

Application Reference

Ouyang A et al. Adv Sci (Weinh) 2024; 11 (39) : e2404365 The Hemagglutinin of Influenza A Virus Induces Ferroptosis to Facilitate Viral Replication.
Liu T et al. Viruses 2024; 16 (8) The M2 Protein of the Influenza A Virus Interacts with PEX19 to Facilitate Virus Replication by Disrupting the Function of Peroxisome.
Wang X et al. Virulence 2024; 15 (1) : 2301242 Immunosuppressants exert antiviral effects against influenza A(H1N1)pdm09 virus via inhibition of nucleic acid synthesis, mRNA splicing, and protein stability.
WK Cho et al. J Funct ?Foods 2024; 112 : 105940 Hoveniae Semen Seu Fructus water extract inhibits influenza A virus infection
Application : ICC/IF
Choi S et al. Sci Adv 2023; 9 (4) : eade2708 Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles.
Application : EM
Show More +
Submit a Reference

REVIEW

Influenza A virus M2 (matrix protein) antibody Cat. No. GTX125951

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951
Date : Chen-I Hsu submitted on 16-Sep-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 25°C, 1Hr
Secondary Antibody : GTX213110-01, 1:2000, 25°C, 1Hr
Lane Description : A549 cells were infected with Influenza A (WSN) (MOI=0.1) and harvested at 6, 24, 48 hours postinfection.
Submit a Review
Application Reference
Ouyang A et al. Adv Sci (Weinh) 2024; 11 (39):e2404365 The Hemagglutinin of Influenza A Virus Induces Ferroptosis to Facilitate Viral Replication.
Liu T et al. Viruses 2024; 16 (8) The M2 Protein of the Influenza A Virus Interacts with PEX19 to Facilitate Virus Replication by Disrupting the Function of Peroxisome.
Wang X et al. Virulence 2024; 15 (1):2301242 Immunosuppressants exert antiviral effects against influenza A(H1N1)pdm09 virus via inhibition of nucleic acid synthesis, mRNA splicing, and protein stability.
WK Cho et al. J Funct ?Foods 2024; 112:105940 Hoveniae Semen Seu Fructus water extract inhibits influenza A virus infection
Application : ICC/IF
Choi S et al. Sci Adv 2023; 9 (4):eade2708 Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles.
Application : EM
Han YJ et al. J Biomed Sci 2023; 30 (1):10 Targeting influenza A virus by splicing inhibitor herboxidiene reveals the importance of subtype-specific signatures around splice sites.
Wu W et al. Front Cell Infect Microbiol 2022; 12:904775 MicroRNA let-7 Suppresses Influenza A Virus Infection by Targeting RPS16 and Enhancing Type I Interferon Response.
Li Q et al. Adv Sci (Weinh) 2022; 9 (34):e2203088 SRSF5-Mediated Alternative Splicing of M Gene is Essential for Influenza A Virus Replication: A Host-Directed Target Against Influenza Virus.
Guangwen Wang et al. PLoS Pathog 2022; 18 (4):e1010446 PIAS1-mediated SUMOylation of influenza A virus PB2 restricts viral replication and virulence.
Xin Mao et al. Evid Based Complement Alternat Med 2021; 2021:5547424 Essential Oil-Rich Chinese Formula Luofushan-Baicao Oil Inhibits the Infection of Influenza A Virus through the Regulation of NF-κB P65 and IRF3 Activation.
Application : WB
Ulferts R et al. Cell Rep 2021; 37 (4):109899 Subtractive CRISPR screen identifies the ATG16L1/vacuolar ATPase axis as required for non-canonical LC3 lipidation.
Hara H et al. Sci Rep 2021; 11 (1):10109 Neurotropic influenza A virus infection causes prion protein misfolding into infectious prions in neuroblastoma cells.
Application : WB
Kwon EB et al. Molecules 2021; 26 (4) 8-O-(E-p-methoxycinnamoyl)harpagide Inhibits Influenza A Virus Infection by Suppressing Intracellular Calcium.
Application : WB
Mruk K et al. Development 2020; 147 (12) Targeted cell ablation in zebrafish using optogenetic transcriptional control.
Zhu Y et al. Sci Adv 2020; 6 (25):eaaz5764 Human TRA2A determines influenza A virus host adaptation by regulating viral mRNA splicing.
Zhang Q et al. Microbes Infect 2020; (Epub) SNW1 interacts with IKKγ to positively regulate antiviral innate immune responses against influenza A virus infection.
Application : WB
Chida J et al. PLoS Pathog 2020; 16 (8):e1008823 Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice.
Application : WB
Omi J et al. Nat Commun 2020; 11 (1):162 The inducible amphisome isolates viral hemagglutinin and defends against influenza A virus infection.
Application : WB
Wang R et al. Autophagy 2019; 15 (7):1163-1181 Influenza M2 protein regulates MAVS-mediated signaling pathway through interacting with MAVS and increasing ROS production.
Wang R et al. J Virol 2019; 93 (4) Autophagy Promotes Replication of Influenza A Virus In Vitro.
Ren X et al. Front Microbiol 2019; 10:1625 Avian Influenza A Virus Polymerase Recruits Cellular RNA Helicase eIF4A3 to Promote Viral mRNA Splicing and Spliced mRNA Nuclear Export.
Application : WB
Hao X et al. Transbound Emerg Dis 2019; (Epub) The PB2 and M genes are critical for the superiority of genotype S H9N2 virus to genotype H in optimizing viral fitness of H5Nx and H7N9 avian influenza viruses in mice.
Application : WB
Karen Mruk et al. bioRxiv 2019; (Epub) Targeted cell ablation in zebrafish using optogenetic transcriptional control.
Application : ICC/IF
Choi JG et al. Sci Rep 2019; 9 (1):12132 Antiviral activity of ethanol extract of Geranii Herba and its components against influenza viruses via neuraminidase inhibition.
Application : WB
Hao X et al. Virology 2019; 535:218-226 The PB2 and M genes of genotype S H9N2 virus contribute to the enhanced fitness of H5Nx and H7N9 avian influenza viruses in chickens.
Application : WB
Lin HT et al. Sci Rep 2018; 8 (1):17242 Grail attenuates influenza A virus infection and pathogenesis by inhibiting viral nucleoprotein.
Application : WB
Wohlgemuth N et al. J Virol 2018; (Epub) Influenza A virus M2 protein apical targeting is required for efficient virus replication.
Chida J et al. PLoS Pathog 2018; 14 (5):e1007049 Prion protein protects mice from lethal infection with influenza A viruses.
Application : WB
Holzberg M et al. Front Microbiol 2017; 8:2426 Vemurafenib Limits Influenza A Virus Propagation by Targeting Multiple Signaling Pathways.
Su WC et al. J Virol 2017; (Epub) Ubiquitination of the Cytoplasmic Domain of Influenza A Virus M2 Protein is Crucial for Production of Infectious Virus Particles.
Qian W et al. Dev Comp Immunol 2017; 78:43113 duck * regulatory factor 1 acts as a positive regulator in duck innate antiviral response.
Application : WB
Choi J et al. Front. Pharmacol. 2017; (Epub) Eupatorium fortunei and Its Components Increase Antiviral Immune Responses against RNA Viruses.
Application : ICC/IF
Zhu P et al. J Virol 2016; (Epub) Host cellular protein TRAPPC6AΔ interacts with influenza A virus M2 protein and regulates viral propagation by modulating M2 trafficking.
Application : WB
Perwitasari O et al. J Virol 2014; 88 (17):10228-43 Verdinexor, a novel selective inhibitor of nuclear export, reduces influenza a virus replication in vitro and in vivo.
Application : ICC/IF
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169