Influenza A virus Nucleoprotein antibody [GT1236]

Cat. No. GTX629633

Reference 12

Host	Mouse
Clonality	Monoclonal
Clone Name	GT1236
Isotype	IgG2b
Application	WB, ICC/IF, IHC-Fr, ELISA, Sandwich ELISA
Reactivity	Influenza A virus
Package	100 μl ($399), 25 μl ($169)

See all Influenza A virus Nucleoprotein products

View product citations for antibody GTX629633 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-Fr	Assay dependent
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture: GTX629633, Detection: GTX125989　or Capture: GTX125989, Detection: GTX629633

Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

WSN virus infected DF1 cells (10 hr)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein of Influenza A virus Nucleoprotein (A/WSN/1933(H1N1). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888128

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza A Virus Nucleoprotein , Influenza A Virus NP

Research Area

DATA IMAGES

GTX629633 ELISA Image

Sandwich ELISA detection of non-transfected and Influenza A virus nucleoprotein transfected 293T whole cell extracts using Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody (GTX125989) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX629633 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza A virus NP (nucleoprotein) protein, DDDDK tag (GTX135868-pro) using Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody (GTX125989) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX629633 WB Image

Influenza A Virus NP antibody [GT1236] detects Influenza A Virus NP protein by Western blot analysis.
A. 5 μg DF1 whole cell lysate/extract
B. 5 μg whole cell lysate/extract of WSN virus infected DF1 cells (10 hr)
7.5 % SDS-PAGE
Influenza A Virus NP antibody [GT1236] (GTX629633) dilution: 1:5000

GTX629633 ICC/IF Image

Influenza A Virus NP antibody [GT1236] detects Influenza A Virus NP protein by immunofluorescent analysis.
Sample: DF1 cells infected with Influenza A virus (WSN) .
Green: Influenza A Virus NP protein stained by Influenza A Virus NP antibody [GT1236] (GTX629633) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX629633 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza A virus NP (nucleoprotein) protein, DDDDK tag (GTX135868-pro) using Influenza A virus NP (nucleoprotein) antibody (GTX125989) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX629633 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody [GT1236] (GTX629633) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Cai Q et al. Cell Biosci 2024; 14 (1) : 23 Interferon-stimulated gene PVRL4 broadly suppresses viral entry by inhibiting viral-cellular membrane fusion.

Yang H et al. PLoS Pathog 2023; 19 (7) : e1011489 The synergistic effect of residues 32T and 550L in the PA protein of H5 subtype avian influenza virus contributes to viral pathogenicity in mice.

Yang H et al. Nat Commun 2022; 13 (1) : 6288 The influenza virus PB2 protein evades antiviral innate immunity by inhibiting JAK1/STAT signalling.

Application : WB

Lee JH et al. Viruses 2021; 13 (9) Nonstructural Protein NS1 of Influenza Virus Disrupts Mitochondrial Dynamics and Enhances Mitophagy via ULK1 and BNIP3.

Application : WB

Zhang B et al. Nat Biomed Eng 2021; 5 (11) : 1288-1305 Site-specific PEGylation of interleukin-2 enhances immunosuppression via the sustained activation of regulatory T cells.

Application : IHC-Fr

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REVIEW

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Application Reference

Cai Q et al. Cell Biosci 2024; 14 (1):23 Interferon-stimulated gene PVRL4 broadly suppresses viral entry by inhibiting viral-cellular membrane fusion.

Yang H et al. PLoS Pathog 2023; 19 (7):e1011489 The synergistic effect of residues 32T and 550L in the PA protein of H5 subtype avian influenza virus contributes to viral pathogenicity in mice.

Yang H et al. Nat Commun 2022; 13 (1):6288 The influenza virus PB2 protein evades antiviral innate immunity by inhibiting JAK1/STAT signalling.

Application : WB

Lee JH et al. Viruses 2021; 13 (9) Nonstructural Protein NS1 of Influenza Virus Disrupts Mitochondrial Dynamics and Enhances Mitophagy via ULK1 and BNIP3.

Application : WB

Zhang B et al. Nat Biomed Eng 2021; 5 (11):1288-1305 Site-specific PEGylation of interleukin-2 enhances immunosuppression via the sustained activation of regulatory T cells.

Application : IHC-Fr

Melano I et al. Viruses 2021; 13 (7) Effects of Basic Amino Acids and Their Derivatives on SARS-CoV-2 and Influenza-A Virus Infection.

Application : WB