Non-infected (–) and infected (+) H1299 whole cell extracts (5 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody (GTX125989) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody (GTX125989) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Influenza A (H1N1) viral lysate (0.1 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody (GTX125989) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sandwich ELISA detection of recombinant full-length Influenza A virus NP (nucleoprotein) protein, DDDDK tag (GTX135868-pro) using Influenza A virus NP (nucleoprotein) antibody (GTX125989) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Influenza A viral lysate (1 ug & 1ng μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody (GTX125989) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-infected (–) and infected (+) DF1 whole cell extracts (5 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody (GTX125989) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sandwich ELISA detection of non-transfected and Influenza A virus nucleoprotein transfected 293T whole cell extracts using Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody (GTX125989) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Sandwich ELISA detection of recombinant full-length Influenza A virus NP (nucleoprotein) protein, DDDDK tag (GTX135868-pro) using Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody (GTX125989) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Immunofluorescent analysis of Influenza virus infected cells using Influenza A virus Nucleoprotein antibody antibody (GTX125989).
Sample: Influenza A and B virus infected cells slide.
Green: Influenza A virus Nucleoprotein antibody antibody (GTX125989) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Influenza A virus lysate (H1N1) infected 293T whole cell extract was separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody (GTX125989) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Influenza A virus Nucleoprotein antibody detects Influenza A virus Nucleoprotein protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Influenza A virus Nucleoprotein stained by Influenza A virus Nucleoprotein antibody (GTX125989) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

The data was published in the journal Virol J in 2020. PMID: 32321537

The data was published in the journal Virol J in 2020. PMID: 32321537

The data was published in the journal Front Immunol in 2017. PMID: 29312300

The data was published in the journal Front Immunol in 2017. PMID: 29312300

The data was published in the journal Oncotarget in 2018. PMID: 29492200

The data was published in the journal J Virol in 2018.PMID: 30209172

The data was published in the journal J Virol in 2018.PMID: 30209172

The data was published in the journal Front Immunol in 2017. PMID: 29312300

The data was published in the journal Front Immunol in 2017. PMID: 29312300

The data was published in the journal Front Immunol in 2017. PMID: 29312300

The data was published in the journal Front Immunol in 2017. PMID: 29312300

The data was published in the journal Sci Rep in 2017. PMID: 28842649

The data was published in the journal Front Immunol in 2018.PMID: 29904383

The data was published in the journal J Virol in 2018.PMID: 30209172

The data was published in the journal Sci Rep in 2015.PMID: 26030097

The data was published in the journal Front Immunol in 2018.PMID: 29904383

The data was published in the journal J Virol in 2018.PMID: 30209172

The data was published in the journal Front Immunol in 2018.PMID: 29904383

The data was published in the 2022 in Cell Death Discov. PMID: 35246529