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Influenza A virus NS1 (nonstructural protein) antibody

Orthogonal
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

Cat. No. GTX125990

Reference 49
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Influenza A virus
Package
100 μl ($399),
25 μl ($169)
See all Influenza A virus NS1 (nonstructural protein) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Positive Control

WSN-infected DF-1 cell s , H1N1-infected H1299 cell

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein of Influenza A virus NS1 (nonstructural protein) (A/WSN/1933(H1N1). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170327

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza A Virus Non-structural protein 1 , Influenza A Virus NS1

Research Area

DATA IMAGES

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

Orthogonal

Influenza A viral lysate and Influenza B viral lysate (0.1 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus NS1 (nonstructural protein) antibody (GTX125990) diluted at 1:440. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125990

GTX125990 ICC/IF Image

Influenza A Virus NS1 antibody detects NS1 (H1N1) protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: NS1 (H1N1) protein stained by Influenza A Virus NS1 antibody (GTX125990) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

Influenza A virus NS1 (nonstructural protein) antibody detects Influenza A virus NS1 (nonstructural protein) protein by western blot analysis. Non-infected (-) and Influenza A virus (H1N1)-infected (+) H1299 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus NS1 (nonstructural protein) antibody (GTX125990) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

Sample (10 μg of whole cell lysate)
A: mock DF-1 cell lysates
B: WSN-infected DF-1 cell lysates
12% SDS PAGE
GTX125990 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus NS1 (nonstructural protein) antibody (GTX125990) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

Orthogonal

The data was published in the journal Viruses in 2019.PMID: 31500389

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

The data was published in the journal PLoS Pathog in 2018.PMID: 29723291

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

The data was published in the journal Nat Commun in 2019. PMID: 31604929

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

The data was published in the journal Front Microbiol in 2018. PMID: 30258431

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

The data was published in the journal Nat Commun in 2019. PMID: 31604929

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

The data was published in the journal Front Microbiol in 2018. PMID: 30258431

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

The data was published in the journal Front Microbiol in 2018. PMID: 30258431

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

The data was published in the journal Front Microbiol in 2018. PMID: 30258431

REFERENCE

Application Reference

Ouyang A et al. Adv Sci (Weinh) 2024; 11 (39) : e2404365 The Hemagglutinin of Influenza A Virus Induces Ferroptosis to Facilitate Viral Replication.
YJ Li et al. PLoS Pathog 2024; 20 (5) : e1012279 OTUB1 contributes to the stability and function of Influenza A virus NS2
Li Q et al. Viruses 2024; 16 (5) HACD3 Prevents PB1 from Autophagic Degradation to Facilitate the Replication of Influenza A Virus.
Q Li et al. Viruses 2024; 16 (5) : 702 HACD3 Prevents PB1 from Autophagic Degradation to Facilitate the Replication of Influenza A Virus
Wang X et al. Virulence 2024; 15 (1) : 2301242 Immunosuppressants exert antiviral effects against influenza A(H1N1)pdm09 virus via inhibition of nucleic acid synthesis, mRNA splicing, and protein stability.
Show More +
Submit a Reference

REVIEW

Influenza A virus NS1 (nonstructural protein) antibody Cat. No. GTX125990

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Date : Anonymous submitted on 06-Nov-2014
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : Influenza virus-infected monkey vero cells
Amount used : 20μg
Blocking : 5% skim milk, 25°C, 1Hr
Submit a Review
Application Reference
Ouyang A et al. Adv Sci (Weinh) 2024; 11 (39):e2404365 The Hemagglutinin of Influenza A Virus Induces Ferroptosis to Facilitate Viral Replication.
YJ Li et al. PLoS Pathog 2024; 20 (5):e1012279 OTUB1 contributes to the stability and function of Influenza A virus NS2
Li Q et al. Viruses 2024; 16 (5) HACD3 Prevents PB1 from Autophagic Degradation to Facilitate the Replication of Influenza A Virus.
Q Li et al. Viruses 2024; 16 (5):702 HACD3 Prevents PB1 from Autophagic Degradation to Facilitate the Replication of Influenza A Virus
Wang X et al. Virulence 2024; 15 (1):2301242 Immunosuppressants exert antiviral effects against influenza A(H1N1)pdm09 virus via inhibition of nucleic acid synthesis, mRNA splicing, and protein stability.
WK Cho et al. J Funct ?Foods 2024; 112:105940 Hoveniae Semen Seu Fructus water extract inhibits influenza A virus infection
Application : ICC/IF
Zhang L et al. J Virol 2023; 97 (5):e0033723 Fine Regulation of Influenza Virus RNA Transcription and Replication by Stoichiometric Changes in Viral NS1 and NS2 Proteins.
Kleinehr J et al. PLoS Pathog 2023; 19 (7):e1010986 Glycolytic interference blocks influenza A virus propagation by impairing viral polymerase-driven synthesis of genomic vRNA.
Zhu Y et al. PLoS Pathog 2023; 19 (4):e1011305 N6-methyladenosine reader protein YTHDC1 regulates influenza A virus NS segment splicing and replication.
Vicary AC et al. PLoS Pathog 2023; 19 (4):e1010943 Maximal interferon induction by influenza lacking NS1 is infrequent owing to requirements for replication and export.
Seoane R et al. Front Cell Infect Microbiol 2022; 12:960138 eIF5A is activated by virus infection or dsRNA and facilitates virus replication through modulation of interferon production.
Application : WB
Lee MC et al. Front Cell Infect Microbiol 2022; 12:998584 Influenza A virus NS1 protein represses antiviral immune response by hijacking NF-κB to mediate transcription of type III IFN.
Ling YH et al. Front Microbiol 2022; 13:895779 Nucleoporin 85 interacts with influenza A virus PB1 and PB2 to promote its replication by facilitating nuclear import of ribonucleoprotein.
Jiang J et al. iScience 2022; 25 (7):104607 LncNSPL facilitates influenza A viral immune escape by restricting TRIM25-mediated K63-linked RIG-I ubiquitination.
Huapeng Feng et al. Viruses 2022; 14 (4) KAP1 Positively Modulates Influenza A Virus Replication by Interacting with PB2 and NS1 Proteins in Human Lung Epithelial Cells.
Application : WB
Amol Patil et al. J Virol 2021; 95 (6) Phosphorylation of Influenza A Virus NS1 at Serine 205 Mediates Its Viral Polymerase-Enhancing Function.
Application : WB
Meng-Ting Huang et al. Viruses 2021; 13 (5) Dual R108K and G189D Mutations in the NS1 Protein of A/H1N1 Influenza Virus Counteract Host Innate Immune Responses.
Application : WB
Vanessa Gerlt et al. Int J Mol Sci 2021; 22 (20) Cellular Protein Phosphatase 2A Regulates Cell Survival Mechanisms in Influenza A Virus Infection.
Application : WB
Tseng YY et al. Viruses 2021; 13 (10) Interaction between NS1 and Cellular MAVS Contributes to NS1 Mitochondria Targeting.
Application : ICC/IF
Nacken W et al. mBio 2021; 12 (5):e0219621 The Effector Domain of the Influenza A Virus Nonstructural Protein NS1 Triggers Host Shutoff by Mediating Inhibition and Global Deregulation of Host Transcription When Associated with Specific Structures in the Nucleus.
Application : WB
Liu X et al. Nat Commun 2021; 12 (1):4427 MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.
Application : WB
Burke JM et al. Sci Adv 2021; 7 (23) RNase L limits host and viral protein synthesis via inhibition of mRNA export.
Application : ICC/IF
Sarto J.D. et al. bioRxiv 2021; (Epub) Phosphorylation of JIP4 at S730 presents anti-viral properties against influenza A virus infection.
Kwon EB et al. Molecules 2021; 26 (4) 8-O-(E-p-methoxycinnamoyl)harpagide Inhibits Influenza A Virus Infection by Suppressing Intracellular Calcium.
Application : WB
Sha TW et al. Virol J 2020; 17 (1):107 Influenza A virus NS1 optimises virus infectivity by enhancing genome packaging in a dsRNA-binding dependent manner.
Zhu Y et al. Sci Adv 2020; 6 (25):eaaz5764 Human TRA2A determines influenza A virus host adaptation by regulating viral mRNA splicing.
Du Y et al. Nat Commun 2020; 11 (1):2449 mRNA display with library of even-distribution reveals cellular interactors of influenza virus NS1.
Chida J et al. PLoS Pathog 2020; 16 (8):e1008823 Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice.
Application : WB
Zhang T et al. Cell 2020; 180 (6):1115-1129 Influenza Virus Z-RNAs Induce ZBP1-Mediated Necroptosis.
Application : WB
Chao TL et al. Front Microbiol 2019; 10:2942 Characterization of Influenza A Virus Infection in Mouse Pulmonary Stem/Progenitor Cells.
Zhang X et al. Viruses 2019; 11 (9) Brevilin A, a Sesquiterpene Lactone, Inhibits the Replication of Influenza A Virus In Vitro and In Vivo.
Application : WB
Ren X et al. Front Microbiol 2019; 10:1625 Avian Influenza A Virus Polymerase Recruits Cellular RNA Helicase eIF4A3 to Promote Viral mRNA Splicing and Spliced mRNA Nuclear Export.
Application : WB
Furusawa Y et al. MBio 2019; 10 (5) Influenza Virus Polymerase Mutation Stabilizes a Foreign Gene Inserted into the Virus Genome by Enhancing the Transcription/Replication Efficiency of the Modified Segment.
Application : WB
Moriyama M et al. Nat Commun 2019; 10 (1):4624 Influenza A virus M2 protein triggers mitochondrial DNA-mediated antiviral immune responses.
Application : ICC/IF
Choi JG et al. Sci Rep 2019; 9 (1):12132 Antiviral activity of ethanol extract of Geranii Herba and its components against influenza viruses via neuraminidase inhibition.
Application : WB
Zhang J et al. Eur J Pharm Sci 2019; 133:86-94 Identification of NMS-873, an allosteric and specific p97 inhibitor, as a broad antiviral against both influenza A and B viruses.
Application : WB
Sethy B et al. J Med Chem 2019; 62 (5):2390-2403 Design, Synthesis, and Biological Evaluation of Itaconic Acid Derivatives as Potential Anti-Influenza Agents.
Application : WB
Li J et al. Biochem J 2019; 476 (3):467-481 MOV10 sequesters the RNP of influenza A virus in the cytoplasm and is antagonized by viral NS1 protein.
Application : WB
Russell AB et al. Elife 2018; 7 Extreme heterogeneity of influenza virus infection in single cells.
Chiba S et al. Front Microbiol 2018; 9:2192 The Cellular DExD/H-Box RNA Helicase UAP56 Co-localizes With the Influenza A Virus NS1 Protein.
Lin HT et al. Sci Rep 2018; 8 (1):17242 Grail attenuates influenza A virus infection and pathogenesis by inhibiting viral nucleoprotein.
Application : WB
Chida J et al. PLoS Pathog 2018; 14 (5):e1007049 Prion protein protects mice from lethal infection with influenza A viruses.
Application : WB
Coates BM et al. J Immunol 2018; (Epub) Inflammatory Monocytes Drive Influenza A Virus-Mediated Lung Injury in Juvenile Mice.
Application : IHC-P
Hu Y et al. Int J Mol Sci 2017; 18 (9) Chemical Genomics Approach Leads to the Identification of Hesperadin, an Aurora B Kinase Inhibitor, as a Broad-Spectrum Influenza Antiviral.
Application : WB
Hu Y et al. Antiviral Res 2017; 145:103-113 Discovery of dapivirine, a nonnucleoside HIV-1 reverse transcriptase inhibitor, as a broad-spectrum antiviral against both influenza A and B viruses.
Application : WB
Hsu SF et al. J Virol 2015; 89 (7):3671-82 A Host Susceptibility Gene, DR1, Facilitates Influenza A Virus Replication by Suppressing Host Innate Immunity and Enhancing Viral RNA Replication.
Application : WB
Tawaratsumida K et al. J Virol 2014; 88 (16):9038-48 Quantitative proteomic analysis of the influenza A virus nonstructural proteins NS1 and NS2 during natural cell infection identifies PACT as an NS1 target protein and antiviral host factor.
Application : WB
Wu NC et al. J Virol 2014; high-throughput identification of loss-of-function mutations for anti-* activity in influenza a virus ns segment.
Application : WB
Strengert M et al. Antioxid Redox Signal 2013; Mucosal Reactive Oxygen Species Are Required for Antiviral Response: Role of Duox in Influenza A Virus Infection.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169