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Influenza A virus H1N1 HA (Hemagglutinin) antibody

Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127357
Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357
Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357
Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357
Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357
Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357
Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357
Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357
Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357

Cat. No. GTX127357

Reference 44
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA, Multiplexing

Reactivity

 Influenza A virus ( H1N1)
Package
100 μl ($399),
25 μl ($169)
See all Influenza A virus HA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
ELISA Assay dependent
Multiplexing Assay dependent
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Influenza A virus H1N1 HA (Hemagglutinin) (A/WSN/1933(H1N1). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2728683

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza A Virus Hemagglutinin , Influenza A Virus HA

Research Area

DATA IMAGES

Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127357

GTX127357 ICC/IF Image

Influenza A Virus H1N1 Hemagglutinin (HA) antibody detects HA (H1N1) protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: HA (H1N1) protein stained by Influenza A Virus H1N1 Hemagglutinin (HA) antibody (GTX127357) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357

GTX127357 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus H1N1 HA (Hemagglutinin) antibody (GTX127357) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357

GTX127357 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus H1N1 HA (Hemagglutinin) antibody (GTX127357) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357

GTX127357 WB Image

The data was published in the journal Sci Rep in 2017.PMID: 28842649

Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357

GTX127357 WB Image

The data was published in the journal Nat Commun in 2019. PMID: 30631056

Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357

GTX127357 WB Image

The data was published in the journal PLoS Pathog in 2017. PMID: 28953980

Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357

GTX127357 WB Image

The data was published in the journal PLoS Pathog in 2017. PMID: 28953980

Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357

GTX127357 WB Image

The data was published in the journal PLoS Pathog in 2017. PMID: 28953980

Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357

GTX127357 WB Image

The data was published in the journal PLoS Pathog in 2017. PMID: 28953980

REFERENCE

Application Reference

Liu X et al. Vaccines (Basel) 2024; 12 (9) A Recombinant Mosaic HAs Influenza Vaccine Elicits Broad-Spectrum Immune Response and Protection of Influenza a Viruses.
H?ring C et al. Front Microbiol 2024; 15 : 1422651 ProcCluster(?) and procaine hydrochloride inhibit the replication of influenza A virus in vitro.
X Liu et al. Vaccines 2024; 12 (9) : 1008 A Recombinant Mosaic HAs Influenza Vaccine Elicits Broad-Spectrum Immune Response and Protection of Influenza a Viruses
L Guzman Ruiz et al. Vaccine 2024; 42 (24) : 126270 Enhancing NA immunogenicity through novel VLP designs
Dupont M et al. Nucleic Acids Res 2024; 52 (12) : 7188-7210 The RBPome of influenza A virus NP-mRNA reveals a role for TDP-43 in viral replication.
Show More +
Submit a Reference

REVIEW

Influenza A virus H1N1 HA (Hemagglutinin) antibody Cat. No. GTX127357

Rating
( Average 4.8 based on 4 users reviews)
Application
Western Blot(WB)
( Average 4.7 based on 3 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357
Date : Anonymous submitted on 05-Mar-2024
Score :
Application Tested : WB
Sample : mouse alveolar lavage fluid with Influenza A virus H1N1 virus (A/California/04) infection
Primary Antibody : 1:5000, °C, Hr
Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357
Date : Chen-I Hsu submitted on 10-Sep-2021
Score :
Application Tested : WB
Sample Species : H1N1
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Secondary Antibody : GTX213110-01, 1:2000, 25°C, 1Hr
Lane Description :
Left : A549 cells were transfected with pcDNA3-HA (WSN) using Lipofectamine3000. The cell was harvested at 24h post-transfection.
Right : A549 cells were transfected with Influenza A virus (WSN) (MOI=0.1) and harvested at 6, 24, 48 hours postinfection.
Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127357
Date : Anonymous submitted on 27-Jul-2018
Score :
Application Tested : ICC/IF
Sample : H1N1 influenza virus A/PR/8/1934 infected MDCK cell
Blocking : 2%BSA in PBS(+), 25°C, 1Hr
Fixation : 3% PFA/100mM HEPES pH7.2 with 2mM MgCl2, 3.6mM CaCl2
Permeabilization /Antigen retrieval : 0.5% TritonX-100 in PBS(+)
Primary Antibody : 1:500, 25°C, 1Hr
Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357
Date : Anonymous submitted on 03-Jul-2015
Score :
Application Tested : WB
Sample Species : H1N1
Sample : influenza virus (A/WSN/33) infected MDCK cells
Amount used : 3 x 10^3 cells
Blocking : 5% nonfat drymilk in TBST, 25°C, overnightHr
Primary Antibody : 1:5000, 25°C, 4Hr
Lane Description : Lane 1: influenza virus A/WSN/33 infected MDCK cells (MOI=1 9hr).Lane 2: MDCK cells.
Submit a Review
Application Reference
Liu X et al. Vaccines (Basel) 2024; 12 (9) A Recombinant Mosaic HAs Influenza Vaccine Elicits Broad-Spectrum Immune Response and Protection of Influenza a Viruses.
H?ring C et al. Front Microbiol 2024; 15:1422651 ProcCluster(?) and procaine hydrochloride inhibit the replication of influenza A virus in vitro.
X Liu et al. Vaccines 2024; 12 (9):1008 A Recombinant Mosaic HAs Influenza Vaccine Elicits Broad-Spectrum Immune Response and Protection of Influenza a Viruses
L Guzman Ruiz et al. Vaccine 2024; 42 (24):126270 Enhancing NA immunogenicity through novel VLP designs
Dupont M et al. Nucleic Acids Res 2024; 52 (12):7188-7210 The RBPome of influenza A virus NP-mRNA reveals a role for TDP-43 in viral replication.
P Chlanda et al. Research Square 2024; Influenza A virus hemagglutinin remodels membranes into a vRNP clustering platform
Application : ICC/IF
Wang X et al. Virulence 2024; 15 (1):2301242 Immunosuppressants exert antiviral effects against influenza A(H1N1)pdm09 virus via inhibition of nucleic acid synthesis, mRNA splicing, and protein stability.
WK Cho et al. J Funct ?Foods 2024; 112:105940 Hoveniae Semen Seu Fructus water extract inhibits influenza A virus infection
Application : ICC/IF
W Shi et al. Emerg Microbes Infect 2023; 12 (2):2270073 ABTB1 facilitates the replication of influenza A virus by counteracting TRIM4-mediated degradation of viral NP protein
Meng X et al. J Virol 2023; 97 (10):e0124523 Palmitoylation of the hemagglutinin of influenza B virus by ER-localized DHHC enzymes 1, 2, 4, and 6 is required for efficient virus replication.
Liu X et al. NPJ Vaccines 2023; 8 (1):132 A mosaic influenza virus-like particles vaccine provides broad humoral and cellular immune responses against influenza A viruses.
Application : ICC/IF
Zhao Z et al. Viruses 2023; 15 (8) Host DNA Demethylation Induced by DNMT1 Inhibition Up-Regulates Antiviral OASL Protein during Influenza a Virus Infection.
Application : WB
Thomas SN et al. iScience 2023; 26 (8):107361 Down syndrome is associated with altered frequency and functioning of tracheal multiciliated cells, and response to influenza virus infection.
Application : ICC/IF
Gao P et al. Sci Adv 2022; 8 (38):eabm6668 Apolipoprotein E mediates cell resistance to influenza virus infection.
Application : WB
Ye Q et al. NPJ Vaccines 2022; 7 (1):84 Rational development of a combined mRNA vaccine against COVID-19 and influenza.
Application : ICC/IF, Multiplexing
Yuchao Chen et al. Sci Adv 2022; 8 (21):eabn3481 Quantitative and ultrasensitive in situ immunoassay technology for SARS-CoV-2 detection in saliva.
Katherine Kaugars et al. Proc Natl Acad Sci U S A 2021; 118 (34) A recombinant herpes virus expressing influenza hemagglutinin confers protection and induces antibody-dependent cellular cytotoxicity.
Application : WB
Luke Lee et al. Res Sq 2021; Quantitative and Ultrasensitive In-situ Immunoassay Technology for SARS-CoV-2 Detection in Saliva.
Yaxin Zhao et al. Int J Mol Sci 2021; 22 (11) CMAS and ST3GAL4 Play an Important Role in the Adsorption of Influenza Virus by Affecting the Synthesis of Sialic Acid Receptors.
Application : WB, ICC/IF
Hara H et al. Sci Rep 2021; 11 (1):10109 Neurotropic influenza A virus infection causes prion protein misfolding into infectious prions in neuroblastoma cells.
Application : WB
Yang F et al. J Virol 2021; 95 (9) Discovery of a Novel Specific Inhibitor Targeting Influenza A Virus Nucleoprotein with Pleiotropic Inhibitory Effects on Various Steps of the Viral Life Cycle.
Application : ICC/IF
Gadalla MR et al. Cell Microbiol 2021; 23 (6):e13322 NS1-mediated upregulation of ZDHHC22 acyltransferase in influenza a virus infected cells.
Application : WB
Zhu Y et al. Sci Adv 2020; 6 (25):eaaz5764 Human TRA2A determines influenza A virus host adaptation by regulating viral mRNA splicing.
Reiter K et al. J Sep Sci 2020; 43 (12):2270-2278 Separation of influenza virus-like particles from baculovirus by polymer-grafted anion exchanger.
Application : WB
Gadalla MR et al. Biochem J 2020; 477 (1):285-303 Hemagglutinin of Influenza A, but not of Influenza B and C viruses is acylated by ZDHHC2, 8, 15 and 20.
Application : WB
Omi J et al. Nat Commun 2020; 11 (1):162 The inducible amphisome isolates viral hemagglutinin and defends against influenza A virus infection.
Application : WB
Lee HS et al. J Infect Dis 2019; (Epub) Matrix metalloproteinase-13 in atherosclerotic plaque is increased by influenza A virus infection.
Application : IHC-P
Choi JG et al. Sci Rep 2019; 9 (1):12132 Antiviral activity of ethanol extract of Geranii Herba and its components against influenza viruses via neuraminidase inhibition.
Application : WB
Zhang K et al. Nat Microbiol 2019; (Epub) Structural basis for influenza virus NS1 protein block of mRNA nuclear export.
Application : WB
Yuan S et al. Nat Commun 2019; 10 (1):120 SREBP-dependent lipidomic reprogramming as a broad-spectrum antiviral target.
Application : WB
Zhang J et al. Eur J Pharm Sci 2019; 133:86-94 Identification of NMS-873, an allosteric and specific p97 inhibitor, as a broad antiviral against both influenza A and B viruses.
Application : WB
Hong JY et al. Antiviral Res 2019; 164:12-22 Highly immunogenic influenza virus-like particles containing B-cell-activating factor (BAFF) for multi-subtype vaccine development.
Application : WB
Lin HT et al. Sci Rep 2018; 8 (1):17242 Grail attenuates influenza A virus infection and pathogenesis by inhibiting viral nucleoprotein.
Application : WB
Mizuta S et al. ChemMedChem 2018; (Epub) Synthesis of Trifluoromethyl-α,β-unsaturated Lactones and Pyrazolinones and Discovery of Influenza Virus Polymerase Inhibitors.
Application : WB
Chida J et al. PLoS Pathog 2018; 14 (5):e1007049 Prion protein protects mice from lethal infection with influenza A viruses.
Application : WB
Koczka K et al. J Biotechnol 2018; 270:61-69 Comparative transcriptome analysis of a Trichoplusia ni cell line reveals distinct host responses to intracellular and secreted protein products expressed by recombinant baculoviruses.
Application : WB
Canale AS et al. J Mol Biol 2018; (Epub) Synonymous mutations at the beginning of the influenza A virus hemagglutinin gene impact experimental fitness.
Application : ICC/IF, ELISA
Xia C et al. J Virol 2018; (Epub) casein kinase 1α mediates degradation of receptors for type i and type ii *s caused by hemagglutinin of influenza a virus.
Application : WB
Su WC et al. J Virol 2017; (Epub) Ubiquitination of the Cytoplasmic Domain of Influenza A Virus M2 Protein is Crucial for Production of Infectious Virus Particles.
Kuss-Duerkop SK et al. PLoS Pathog 2017; 13 (9):e1006635 Influenza virus differentially activates mTORC1 and mTORC2 signaling to maximize late stage replication.
Watanabe K et al. Sci Rep 2017; 7 (1):9500 Structure-based drug discovery for combating influenza virus by targeting the PA-PB1 interaction.
Application : WB
Hu Y et al. Antiviral Res 2017; 145:103-113 Discovery of dapivirine, a nonnucleoside HIV-1 reverse transcriptase inhibitor, as a broad-spectrum antiviral against both influenza A and B viruses.
Application : WB
Weisshaar M et al. J Virol 2016; 90 (16):7368-7387 Identification and Characterization of Influenza Virus Entry Inhibitors through Dual Myxovirus High-Throughput Screening.
Application : WB
Xia C et al. J Virol 2015; Hemagglutinin of influenza A virus antagonizes type I IFN responses by inducing degradation of type I IFN receptor 1.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169