Recombinant H3N2 HA protein were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus H3N2 HA (Hemagglutinin) antibody (GTX127363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Influenza A Virus H3N2 Hemagglutinin (HA) antibody detects HA (H3N2) protein by western blot analysis.
A. MDCK cell lysates infected with 4 HAU/ml of A/duck/Alberta/35/1976-like, H1N1
B. MDCK cell lysates infected with 4 HAU/ml of A/duck/Ukraine/1963-like, H3N8
C. MDCK cell lysates infected with 4 HAU/ml of A/mallard/Miyagi/53/1976-like, H5N3
D. MDCK cell lysates infected with 4 HAU/ml of A/duck/Taiwan/33/1993-like, H7N7
E. MDCK cell lysates infected with 4 HAU/ml of A/turkey/Wisconsin/1/1966-like, H9N2
F. MDCK cell lysates infected with 4 HAU/ml of A/PR/8/34, H1N1
G. MDCK cell lysates infected with 4 HAU/ml of A/Taiwan/126/2009, pdmH1N1
H. MDCK cell lysates infected with 4 HAU/ml of A/Taiwan/3446/2002, H3N2
12% SDS-PAGE
Influenza A Virus H3N2 Hemagglutinin (HA) antibody (GTX127363) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Influenza A Virus H3N2 Hemagglutinin (HA) antibody detects Influenza A Virus H3N2 Hemagglutinin (HA) protein on mouse nasal tissue(infect with Influenza Virus A ) by immunohistochemical analysis.
Sample: Paraffin-embedded mouse nasal tissue(infect with Influenza Virus A ).
Influenza A Virus H3N2 Hemagglutinin (HA) antibody (GTX127363) dilution: 1:50.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus H3N2 HA (Hemagglutinin) antibody (GTX127363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Influenza A and Influenza B viral lysate (0.1 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus H3N2 HA (Hemagglutinin) antibody (GTX127363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Sci Rep in 2016. PMID: 27653209

The data was published in the journal Sci Rep in 2016. PMID: 27653209