Influenza A virus H3N2 HA (Hemagglutinin) antibody

Cat. No. GTX127363

Reference 9

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Influenza A virus
Package	100 μl ($399), 25 μl ($169)

See all Influenza A virus HA3 products

View product citations for antibody GTX127363 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:50-1:1000

Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

H3N2 HA recombinant protein

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Influenza A virus H3N2 HA (Hemagglutinin) (A/TW/3446/02(H3N2). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885648

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza A Virus Hemagglutinin , Influenza A Virus HA

Research Area

DATA IMAGES

GTX127363 WB Image

Recombinant H3N2 HA protein were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus H3N2 HA (Hemagglutinin) antibody (GTX127363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127363 IHC-P Image

Influenza A Virus H3N2 Hemagglutinin (HA) antibody detects Influenza A Virus H3N2 Hemagglutinin (HA) protein on mouse nasal tissue(infect with Influenza Virus A ) by immunohistochemical analysis.
Sample: Paraffin-embedded mouse nasal tissue(infect with Influenza Virus A ).
Influenza A Virus H3N2 Hemagglutinin (HA) antibody (GTX127363) dilution: 1:50.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX127363 WB Image

Influenza A Virus H3N2 Hemagglutinin (HA) antibody detects HA (H3N2) protein by western blot analysis.
A. MDCK cell lysates infected with 4 HAU/ml of A/duck/Alberta/35/1976-like, H1N1
B. MDCK cell lysates infected with 4 HAU/ml of A/duck/Ukraine/1963-like, H3N8
C. MDCK cell lysates infected with 4 HAU/ml of A/mallard/Miyagi/53/1976-like, H5N3
D. MDCK cell lysates infected with 4 HAU/ml of A/duck/Taiwan/33/1993-like, H7N7
E. MDCK cell lysates infected with 4 HAU/ml of A/turkey/Wisconsin/1/1966-like, H9N2
F. MDCK cell lysates infected with 4 HAU/ml of A/PR/8/34, H1N1
G. MDCK cell lysates infected with 4 HAU/ml of A/Taiwan/126/2009, pdmH1N1
H. MDCK cell lysates infected with 4 HAU/ml of A/Taiwan/3446/2002, H3N2
12% SDS-PAGE
Influenza A Virus H3N2 Hemagglutinin (HA) antibody (GTX127363) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127363 WB Image

Influenza A and Influenza B viral lysate (0.1 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus H3N2 HA (Hemagglutinin) antibody (GTX127363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127363 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus H3N2 HA (Hemagglutinin) antibody (GTX127363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127363 WB Image

The data was published in the journal Sci Rep in 2016. PMID: 27653209

GTX127363 WB Image

The data was published in the journal Sci Rep in 2016. PMID: 27653209

REFERENCE

Application Reference

Liu X et al. Vaccines (Basel) 2024; 12 (9) A Recombinant Mosaic HAs Influenza Vaccine Elicits Broad-Spectrum Immune Response and Protection of Influenza a Viruses.

X Liu et al. Vaccines 2024; 12 (9) : 1008 A Recombinant Mosaic HAs Influenza Vaccine Elicits Broad-Spectrum Immune Response and Protection of Influenza a Viruses

Liu X et al. NPJ Vaccines 2023; 8 (1) : 132 A mosaic influenza virus-like particles vaccine provides broad humoral and cellular immune responses against influenza A viruses.

Application : ICC/IF

Dorothea Bestle et al. J Virol 2021; 95 (20) : e0090621 Hemagglutinins of Avian Influenza Viruses Are Proteolytically Activated by TMPRSS2 in Human and Murine Airway Cells.

Sch?n J et al. PLoS Pathog 2021; 17 (4) : e1009490 Neuraminidase-associated plasminogen recruitment enables systemic spread of natural avian Influenza viruses H3N1.

Application : WB

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REVIEW

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Application Reference

Liu X et al. Vaccines (Basel) 2024; 12 (9) A Recombinant Mosaic HAs Influenza Vaccine Elicits Broad-Spectrum Immune Response and Protection of Influenza a Viruses.

X Liu et al. Vaccines 2024; 12 (9):1008 A Recombinant Mosaic HAs Influenza Vaccine Elicits Broad-Spectrum Immune Response and Protection of Influenza a Viruses

Liu X et al. NPJ Vaccines 2023; 8 (1):132 A mosaic influenza virus-like particles vaccine provides broad humoral and cellular immune responses against influenza A viruses.

Application : ICC/IF

Dorothea Bestle et al. J Virol 2021; 95 (20):e0090621 Hemagglutinins of Avian Influenza Viruses Are Proteolytically Activated by TMPRSS2 in Human and Murine Airway Cells.

Sch?n J et al. PLoS Pathog 2021; 17 (4):e1009490 Neuraminidase-associated plasminogen recruitment enables systemic spread of natural avian Influenza viruses H3N1.

Application : WB

Harbig A et al. J Biol Chem 2020; 295 (33):11388-11407 Transcriptome profiling and protease inhibition experiments identify proteases that activate H3N2 influenza A and influenza B viruses in murine airways.

Limburg H et al. J Virol 2019; TMPRSS2 is the major activating protease of influenza A virus in primary human airway cells and influenza B virus in human type II pneumocytes.

Diot C et al. Sci Rep 2016; 6:33763 Influenza A Virus Polymerase Recruits the RNA Helicase DDX19 to Promote the Nuclear Export of Viral mRNAs.