GeneTex

  • Country / Location Selection

United States (US)

Influenza A virus PA protein antibody

Anti-Influenza A virus PA protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125932
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932

Cat. No. GTX125932

Reference 34

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Influenza A virus
Package
100 μl ($399),
25 μl ($169)
See all Influenza A virus PA protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

WSN virus infected DF1

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Influenza A virus PA (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175740

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza A Virus Polymerase acidic protein , Influenza A Virus PA

Research Area

DATA IMAGES

Anti-Influenza A virus PA protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125932

GTX125932 ICC/IF Image

Influenza A Virus PA antibody detects PA protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: PA protein stained by Influenza A Virus PA antibody (GTX125932) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932

GTX125932 WB Image

Sample (5 μg of whole cell lysate)
A: DF1
B: WSN virus infected DF1 10 hr
7.5% SDS PAGE
GTX125932 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932

GTX125932 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PA protein antibody (GTX125932) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932

GTX125932 WB Image

The data was published in the journal Sci Rep in 2015.PMID: 26030097

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932

GTX125932 WB Image

The data was published in the journal Nat Commun in 2017.PMID: 28169297

REFERENCE

Application Reference

X Li et al. Emerg Microbes Infect 2024; 13 (1) : 2387439 The PB2 I714S mutation influenced mammalian adaptation of the H3N2 canine influenza virus by interfering with nuclear import efficiency and RNP complex assembly
Dupont M et al. Nucleic Acids Res 2024; 52 (12) : 7188-7210 The RBPome of influenza A virus NP-mRNA reveals a role for TDP-43 in viral replication.
Chen YL et al. Viruses 2024; 16 (4) Novel Anti-Viral Properties of the Herbal Extract of Davallia mariesii against Influenza A Virus.
J Bougon et al. J Virol 2024; : e0190123 Influenza A virus NS1 effector domain is required for PA-Xmediated host shutoff in infected cells
Application : WB
YL Chen et al. Viruses 2024; 16 : 523 Novel Anti-Viral Properties of the Herbal Extract of Davallia mariesii against Influenza A Virus
Show More +
Submit a Reference

REVIEW

There are currently no reviews for Influenza A virus PA protein antibody (GTX125932). Be the first to share your experience with this product.
Submit a Review
Application Reference
X Li et al. Emerg Microbes Infect 2024; 13 (1):2387439 The PB2 I714S mutation influenced mammalian adaptation of the H3N2 canine influenza virus by interfering with nuclear import efficiency and RNP complex assembly
Dupont M et al. Nucleic Acids Res 2024; 52 (12):7188-7210 The RBPome of influenza A virus NP-mRNA reveals a role for TDP-43 in viral replication.
Chen YL et al. Viruses 2024; 16 (4) Novel Anti-Viral Properties of the Herbal Extract of Davallia mariesii against Influenza A Virus.
J Bougon et al. J Virol 2024;:e0190123 Influenza A virus NS1 effector domain is required for PA-Xmediated host shutoff in infected cells
Application : WB
YL Chen et al. Viruses 2024; 16:523 Novel Anti-Viral Properties of the Herbal Extract of Davallia mariesii against Influenza A Virus
Zhang L et al. J Virol 2024; 98 (1):e0116623 Twenty natural amino acid substitution screening at the last residue 121 of influenza A virus NS2 protein reveals the critical role of NS2 in promoting virus genome replication by coordinating with viral polymerase.
Yang H et al. PLoS Pathog 2023; 19 (7):e1011489 The synergistic effect of residues 32T and 550L in the PA protein of H5 subtype avian influenza virus contributes to viral pathogenicity in mice.
Kleinehr J et al. PLoS Pathog 2023; 19 (7):e1010986 Glycolytic interference blocks influenza A virus propagation by impairing viral polymerase-driven synthesis of genomic vRNA.
Chen J et al. Virol Sin 2023; 38 (1):128-141 Nonmuscle myosin IIA promotes the internalization of influenza A virus and regulates viral polymerase activity through interacting with nucleoprotein in human pulmonary cells.
Application : WB
G?nl F et al. Nat Commun 2023; 14 (1):787 The ubiquitination landscape of the influenza A virus polymerase.
Application : WB, ICC/IF
Chen Y et al. Influenza Other Respir Viruses 2023; 17 (3):e13112 Antiviral effect and mechanism of Phillyrin and its reformulated FS21 against influenza.
Application : WB
Yang ML et al. J Biomed Sci 2023; 30 (1):14 Upregulation of galectin-3 in influenza A virus infection promotes viral RNA synthesis through its association with viral PA protein.
Application : WB, ICC/IF
Zheng H et al. J Virol 2022; 96 (12):e0049422 G Protein Subunit β1 Facilitates Influenza A Virus Replication by Promoting the Nuclear Import of PB2.
Application : WB
Jianyuan Zhao et al. Nat Commun 2022; 13 (1):2079 An anti-influenza A virus microbial metabolite acts by degrading viral endonuclease PA.
Application : WB
Xiaoman Liu et al. Nat Commun 2021; 12 (1):4427 MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.
Hatakeyama D et al. FEBS J 2021; (Epub) Acetylation of the influenza A virus polymerase subunit PA in the N-terminal domain positively regulates its endonuclease activity.
Application : WB
Van den Hoecke S et al. mBio 2021; 12 (4):e0074521 In Vivo Therapy with M2e-Specific IgG Selects for an Influenza A Virus Mutant with Delayed Matrix Protein 2 Expression.
Application : WB
Slaine PD et al. J Virol 2021; Thiopurines activate an antiviral unfolded protein response that blocks influenza A virus glycoprotein accumulation.
Application : WB
Walker AP et al. bioRxiv 2020; (Epub) Enisamium is a small molecule inhibitor of the influenza A virus and SARS-CoV-2 RNA polymerases.
Rigby RE et al. Sci Rep 2019; 9 (1):7216 PA-X antagonises MAVS-dependent accumulation of early type I interferon messenger RNAs during influenza A virus infection.
Zhang J et al. Eur J Pharm Sci 2019; 133:86-94 Identification of NMS-873, an allosteric and specific p97 inhibitor, as a broad antiviral against both influenza A and B viruses.
Application : WB
Sethy B et al. J Med Chem 2019; 62 (5):2390-2403 Design, Synthesis, and Biological Evaluation of Itaconic Acid Derivatives as Potential Anti-Influenza Agents.
Application : WB
Li J et al. Biochem J 2019; 476 (3):467-481 MOV10 sequesters the RNP of influenza A virus in the cytoplasm and is antagonized by viral NS1 protein.
Application : WB
Kupke SY et al. J Virol 2018; (Epub) A novel type of influenza A virus-derived defective interfering particle with nucleotide substitutions in its genome.
Application : WB
Wang L et al. Nat Commun 2017; 8:13876 Comparative influenza protein interactomes identify the role of plakophilin 2 in virus restriction.
Application : WB
Kuo SM et al. MBio 2017; 8 (3) Inhibition of Avian Influenza A Virus Replication in Human Cells by Host Restriction Factor TUFM Is Correlated with Autophagy.
Application : WB
Xu G et al. Virology 2017; 508:45-53 PA-X protein contributes to virulence of triple-reassortant H1N2 influenza virus by suppressing early immune responses in swine.
Application : WB
Sadewasser A et al. Mol Cell Proteomics 2017; (Epub) Quantitative proteomic approach identifies Vpr binding protein as novel host factor supporting influenza A virus infections in human cells.
Application : WB
Hu J et al. Med Microbiol Immunol 2016; (Epub) iTRAQ-based quantitative proteomics reveals important host factors involved in the high pathogenicity of the H5N1 avian influenza virus in mice.
Application : ICC/IF
Hu J et al. Med Microbiol Immunol 2016; 205 (4):381-95 PA-X-associated early alleviation of the acute lung injury contributes to the attenuation of a highly pathogenic H5N1 avian influenza virus in mice.
Application : ICC/IF
Sun H et al. Sci Rep 2015; 5:10130 The infection of turkeys and chickens by reassortants derived from pandemic H1N1 2009 and avian H9N2 influenza viruses.
Application : WB
Gao H et al. J Gen Virol 2015; 96 (9):2587-94 PA-X is a virulence factor in avian H9N2 influenza virus.
Application : WB
Wu NC et al. PLoS Genet 2015; 11 (7):e1005310 Functional Constraint Profiling of a Viral Protein Reveals Discordance of Evolutionary Conservation and Functionality.
Application : WB
Li W et al. J Virol 2014; (Epub) Interactions between the Influenza A Virus RNA Polymerase Components and Retinoic Acid-Inducible Gene I.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169