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Influenza A virus PB1 protein antibody

Anti-Influenza A virus PB1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125923
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923

Cat. No. GTX125923

Reference 61
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Influenza A virus
Package
100 μl ($399),
25 μl ($169)
See all Influenza A virus PB1 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

DF1 (WSN virus treatment for 10hr)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Influenza A virus PB1 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2753122

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza A Virus Polymerase basic protein 1 , Influenza A Virus PB1

Research Area

DATA IMAGES

Anti-Influenza A virus PB1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125923

GTX125923 ICC/IF Image

Influenza A Virus PB1 antibody detects PB1 protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: PB1 protein stained by Influenza A Virus PB1 antibody (GTX125923) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923

GTX125923 WB Image

Influenza A Virus PB1 antibody detects protein by western blot analysis.
A. 5 μg DF1 whole cell lysate/extract (untreated)
B. 5 μg DF1 whole cell lysate/extract (WSN virus treatment for 10hr)
7.5% SDS-PAGE
Influenza A Virus PB1 antibody (GTX125923) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923

GTX125923 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PB1 protein antibody (GTX125923) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923

GTX125923 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28842649

Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923

GTX125923 WB Image

The data was published in the journal J Virol in 2018.PMID: 30209172

Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923

GTX125923 WB Image

The data was published in the journal J Virol in 2018.PMID: 30209172

Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923

GTX125923 WB Image

The data was published in the journal J Virol in 2018.PMID: 29118119

Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923

GTX125923 WB Image

The data was published in the journal J Virol in 2018.PMID: 29118119

Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923

GTX125923 WB Image

The data was published in the journal J Virol in 2019. PMID: 31068418

REFERENCE

Application Reference

K?nig S et al. Front Cell Infect Microbiol 2024; 14 : 1445428 ProcCluster? and procaine hydrochloride inhibit the growth of Aspergillus species and exert antimicrobial properties during coinfection with influenza A viruses and A. fumigatus in vitro.
H?ring C et al. Front Microbiol 2024; 15 : 1422651 ProcCluster(?) and procaine hydrochloride inhibit the replication of influenza A virus in vitro.
X Li et al. Emerg Microbes Infect 2024; 13 (1) : 2387439 The PB2 I714S mutation influenced mammalian adaptation of the H3N2 canine influenza virus by interfering with nuclear import efficiency and RNP complex assembly
L Cao et al. Emerg Microbes Infect 2024; : 2387910 Inhibition of RAN Attenuates Influenza A Virus Replication and Nucleoprotein Nuclear Export
H Zhang et al. Research Square 2024; Influenza A Virus Employs Nei Endonuclease VIIILike 1 (NEIL1) to Strength vRNP Function and Block IFN-β Production
Show More +
Submit a Reference

REVIEW

Influenza A virus PB1 protein antibody Cat. No. GTX125923

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Date : Anonymous submitted on 19-Apr-2022
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : MDCK cells with/without Influenza A virus (A/Puerto Rico/8/1934) infection
Amount used : 15μg
Blocking : 5% skim milk in 0.1% TBS-T, 20°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Date : Chen-I Hsu submitted on 16-Sep-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Secondary Antibody : GTX213110-01, 1:2000, 25°C, 1Hr
Lane Description : A549 cells were infected with Influenza A (WSN) (MOI=0.1) and harvested at 6, 24, 48 hours postinfection.
Submit a Review
Application Reference
K?nig S et al. Front Cell Infect Microbiol 2024; 14:1445428 ProcCluster? and procaine hydrochloride inhibit the growth of Aspergillus species and exert antimicrobial properties during coinfection with influenza A viruses and A. fumigatus in vitro.
H?ring C et al. Front Microbiol 2024; 15:1422651 ProcCluster(?) and procaine hydrochloride inhibit the replication of influenza A virus in vitro.
X Li et al. Emerg Microbes Infect 2024; 13 (1):2387439 The PB2 I714S mutation influenced mammalian adaptation of the H3N2 canine influenza virus by interfering with nuclear import efficiency and RNP complex assembly
L Cao et al. Emerg Microbes Infect 2024;:2387910 Inhibition of RAN Attenuates Influenza A Virus Replication and Nucleoprotein Nuclear Export
H Zhang et al. Research Square 2024; Influenza A Virus Employs Nei Endonuclease VIIILike 1 (NEIL1) to Strength vRNP Function and Block IFN-β Production
WK Cho et al. J Funct ?Foods 2024; 112:105940 Hoveniae Semen Seu Fructus water extract inhibits influenza A virus infection
Application : ICC/IF
L Ma et al. Virol Sin 2023; S1995-820X (23):00136-0 Mutual antagonism of mouse-adaptation mutations in HA and PA proteins on H9N2 virus replication
Lamb CH et al. bioRxiv 2023; Cas13-based amplification-free quantification of aberrant RNAs during influenza virus infection.
Sheppard CM et al. Nat Commun 2023; 14 (1):6135 An Influenza A virus can evolve to use human ANP32E through altering polymerase dimerization.
Rigby CV et al. Virus Evol 2023; 9 (2):vead056 Evolution of?transient RNA structure-RNA polymerase interactions in?respiratory RNA virus genomes.
Yang H et al. PLoS Pathog 2023; 19 (7):e1011489 The synergistic effect of residues 32T and 550L in the PA protein of H5 subtype avian influenza virus contributes to viral pathogenicity in mice.
Rigby C et al. bioRxiv 2023; Evolution of transient RNA structure-RNA polymerase interactions in respiratory RNA virus genomes.
Chen J et al. Virol Sin 2023; 38 (1):128-141 Nonmuscle myosin IIA promotes the internalization of influenza A virus and regulates viral polymerase activity through interacting with nucleoprotein in human pulmonary cells.
Application : WB
G?nl F et al. Nat Commun 2023; 14 (1):787 The ubiquitination landscape of the influenza A virus polymerase.
Application : WB, ICC/IF
Zheng H et al. J Virol 2022; 96 (12):e0049422 G Protein Subunit β1 Facilitates Influenza A Virus Replication by Promoting the Nuclear Import of PB2.
Application : WB
Li Q et al. Adv Sci (Weinh) 2022; 9 (34):e2203088 SRSF5-Mediated Alternative Splicing of M Gene is Essential for Influenza A Virus Replication: A Host-Directed Target Against Influenza Virus.
French H et al. Sci Adv 2022; 8 (36):eabp8655 Transient RNA structures cause aberrant influenza virus replication and innate immune activation.
Jianyuan Zhao et al. Nat Commun 2022; 13 (1):2079 An anti-influenza A virus microbial metabolite acts by degrading viral endonuclease PA.
Application : WB
Sonja Fernbach et al. Cell Rep 2022; 38 (12):110549 Restriction factor screening identifies RABGAP1L-mediated disruption of endocytosis as a host antiviral defense.
Application : WB
Ma C et al. PLoS Pathog 2022; 18 (2):e1010295 p21 restricts influenza A virus by perturbing the viral polymerase complex and upregulating type I interferon signaling.
Application : WB
Junping Li et al. PLoS Pathog 2021; 17 (2):e1009336 Viral RNA-binding ability conferred by SUMOylation at PB1 K612 of influenza A virus is essential for viral pathogenesis and transmission.
Application : WB, ICC/IF
Amol Patil et al. J Virol 2021; 95 (6) Phosphorylation of Influenza A Virus NS1 at Serine 205 Mediates Its Viral Polymerase-Enhancing Function.
Application : WB
Vanessa Gerlt et al. Int J Mol Sci 2021; 22 (20) Cellular Protein Phosphatase 2A Regulates Cell Survival Mechanisms in Influenza A Virus Infection.
Application : WB
Froggatt HM et al. PLoS Pathog 2021; 17 (9):e1009951 Influenza A virus segments five and six can harbor artificial introns allowing expanded coding capacity.
Application : WB
Liu X et al. Nat Commun 2021; 12 (1):4427 MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.
Application : WB
Deinhardt-Emmer S et al. Biomolecules 2021; 11 (6) Inhibition of Phosphatidylinositol 3-Kinase by Pictilisib Blocks Influenza Virus Propagation in Cells and in Lungs of Infected Mice.
Application : WB
Gong W et al. J Virol 2021; Interaction of NEP with G Protein Pathway Suppressor 2 Facilitates Influenza A Virus Replication by Weakening the Inhibition of GPS2 to RNA Synthesis and Ribonucleoprotein Assembly.
Application : WB
Sarto J.D. et al. bioRxiv 2021; (Epub) Phosphorylation of JIP4 at S730 presents anti-viral properties against influenza A virus infection.
Zhu Y et al. Sci Adv 2020; 6 (25):eaaz5764 Human TRA2A determines influenza A virus host adaptation by regulating viral mRNA splicing.
Sun N et al. Cell Rep 2020; 33 (5):108342 Type-IInterferon-Inducible SERTAD3 Inhibits Influenza A Virus Replication by Blocking the Assembly of Viral RNA Polymerase Complex.
Application : WB
Gao Q et al. J Virol 2020; 95 (2) Eukaryotic Translation Elongation Factor 1 Delta Inhibits the Nuclear Import of the Nucleoprotein and PA-PB1 Heterodimer of Influenza A Virus.
Application : WB
Carrique L et al. Nature 2020; 587 (7835):638-643 Host ANP32A mediates the assembly of the influenza virus replicase.
Application : WB
Chida J et al. PLoS Pathog 2020; 16 (8):e1008823 Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice.
Application : WB
Zhang X et al. Front Microbiol 2020; 11:538355 Avian Chaperonin Containing TCP1 Subunit 5 Supports Influenza A Virus Replication by Interacting With Viral Nucleoprotein, PB1, and PB2 Proteins.
Application : WB
Walker AP et al. J Virol 2020; 94 (13) Mutation of an Influenza Virus Polymerase 3' RNA Promoter Binding Site Inhibits Transcription Elongation.
Application : WB
Hu A et al. Front Microbiol 2020; 11:178 Anthralin Suppresses the Proliferation of Influenza Virus by Inhibiting the Cap-Binding and Endonuclease Activity of Viral RNA Polymerase.
Application : WB
Zhang T et al. Cell 2020; 180 (6):1115-1129 Influenza Virus Z-RNAs Induce ZBP1-Mediated Necroptosis.
Application : WB
Wang R et al. J Virol 2019; 93 (4) Autophagy Promotes Replication of Influenza A Virus In Vitro.
Chao TL et al. Front Microbiol 2019; 10:2942 Characterization of Influenza A Virus Infection in Mouse Pulmonary Stem/Progenitor Cells.
Application : WB
Russell AB et al. J Virol 2019; 93 (14) Single-Cell Virus Sequencing of Influenza Infections That Trigger Innate Immunity.
Hao X et al. Transbound Emerg Dis 2019; (Epub) The PB2 and M genes are critical for the superiority of genotype S H9N2 virus to genotype H in optimizing viral fitness of H5Nx and H7N9 avian influenza viruses in mice.
Application : WB
Choi JG et al. Sci Rep 2019; 9 (1):12132 Antiviral activity of ethanol extract of Geranii Herba and its components against influenza viruses via neuraminidase inhibition.
Application : WB
Arai Y et al. PLoS Pathog 2019; 15 (7):e1007919 PB2 mutations arising during H9N2 influenza evolution in the Middle East confer enhanced replication and growth in mammals.
Application : WB
Hao X et al. Virology 2019; 535:218-226 The PB2 and M genes of genotype S H9N2 virus contribute to the enhanced fitness of H5Nx and H7N9 avian influenza viruses in chickens.
Application : WB
Zheng W et al. Cell Rep 2019; 27 (6):1875-1885.e5 Naproxen Exhibits Broad Anti-influenza Virus Activity in Mice by Impeding Viral Nucleoprotein Nuclear Export.
Application : ICC/IF
Zhang J et al. Eur J Pharm Sci 2019; 133:86-94 Identification of NMS-873, an allosteric and specific p97 inhibitor, as a broad antiviral against both influenza A and B viruses.
Application : WB
Mohl G et al. Antiviral Res 2019; 164:81-90 Novel influenza inhibitors designed to target PB1 interactions with host importin RanBP5.
Li J et al. Biochem J 2019; 476 (3):467-481 MOV10 sequesters the RNP of influenza A virus in the cytoplasm and is antagonized by viral NS1 protein.
Application : WB
Nilsson-Payant BE et al. J Virol 2018; 92 (16) The Surface-Exposed PA 51-72-Loop of the Influenza A Virus Polymerase Is Required for Viral Genome Replication.
Lin HT et al. Sci Rep 2018; 8 (1):17242 Grail attenuates influenza A virus infection and pathogenesis by inhibiting viral nucleoprotein.
Application : WB
Yang C et al. J Virol 2018; (Epub) The nucleolar protein LYAR facilitates ribonucleoprotein assembly of influenza A virus.
Application : WB, ICC/IF
Wang J et al. Viruses 2018; 10 (6) Host Long Noncoding RNA lncRNA-PAAN Regulates the Replication of Influenza A Virus.
Application : WB
Chen SC et al. J Virol 2017; 91 (20) Zinc Finger-Containing Cellular Transcription Corepressor ZBTB25 Promotes Influenza Virus RNA Transcription and Is a Target for Zinc Ejector Drugs.
Oymans J et al. J Virol 2017; (Epub) A mechanism for prime-realignment during influenza A virus replication.
Application : WB
Oymans J et al. bioRxiv 2017; (Epub) A mechanism for prime-realignment during influenza A virus replication.
Application : WB
Watanabe K et al. Sci Rep 2017; 7 (1):9500 Structure-based drug discovery for combating influenza virus by targeting the PA-PB1 interaction.
Application : WB
Kuo SM et al. MBio 2017; 8 (3) Inhibition of Avian Influenza A Virus Replication in Human Cells by Host Restriction Factor TUFM Is Correlated with Autophagy.
Application : WB
Du Y et al. MBio 2016; 7 (6) Annotating Protein Functional Residues by Coupling High-Throughput Fitness Profile and Homologous-Structure Analysis.
Application : ICC/IF
Arai Y et al. PLoS Pathog 2016; 12 (4):e1005583 Novel Polymerase Gene Mutations for Human Adaptation in Clinical Isolates of Avian H5N1 Influenza Viruses.
Application : WB
Su WC et al. J Virol 2015; 89 (22):11245-55 A Nucleolar Protein, Ribosomal RNA Processing 1 Homolog B (RRP1B), Enhances the Recruitment of Cellular mRNA in Influenza Virus Transcription.
Application : WB
Hsu SF et al. J Virol 2015; 89 (7):3671-82 A Host Susceptibility Gene, DR1, Facilitates Influenza A Virus Replication by Suppressing Host Innate Immunity and Enhancing Viral RNA Replication.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169