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Influenza A virus PB2 protein antibody

KO/KD Orthogonal
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

Cat. No. GTX125926

Reference 70
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Influenza A virus, Influenza A virus ( H1N1)
Package
100 μl ($399),
25 μl ($169)
See all Influenza A virus PB2 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

WSN virus infected DF1

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Influenza A virus PB2 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162999

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza A Virus Polymerase basic protein 2 , Influenza A Virus PB2

Research Area

DATA IMAGES

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PB2 protein antibody (GTX125926) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

Sample (5 μg of whole cell lysate)
A: DF1
B: WSN virus infected DF1 10 hr
7.5% SDS PAGE
GTX125926 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PB2 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125926

GTX125926 ICC/IF Image

Influenza A Virus PB2 antibody detects PB2 protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: PB2 protein stained by Influenza A Virus PB2 antibody (GTX125926) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

0.5 μg Influenza A viral lysate (0.5 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PB2 protein antibody (GTX125926) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

Orthogonal

The data was published in the journal J Virol in 2018.PMID: 30209172

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

The data was published in the journal J Virol in 2018.PMID: 30209172

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

The data was published in the journal J Virol in 2018.PMID: 30209172

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

The data was published in the journal J Virol in 2018.PMID: 29118119

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

The data was published in the journal Nat Commun in 2017.PMID: 28169297

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

The data was published in the journal J Virol in 2018.PMID: 30209172

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

The data was published in the journal J Virol in 2019.PMID: 31217244

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

The data was published in the journal J Virol in 2019.PMID: 31217244

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

The data was published in the journal J Virol in 2019.PMID: 31217244

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

The data was published in the journal Elife in 2019. PMID: 31159925

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

The data was published in the journal Elife in 2019. PMID: 31159925

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

The data was published in the journal Elife in 2019. PMID: 31159925

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

The data was published in the journal J Virol in 2019. PMID: 31217244

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

The data was published in the journal J Virol in 2019. PMID: 31217244

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

The data was published in the journal J Virol in 2019. PMID: 31217244

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

The data was published in the journal J Virol in 2019. PMID: 31217244

REFERENCE

Application Reference

G Dugied et al. Mol Cell Proteomics 2024; : 100856 Profiling Cullin4-E3 ligases interactomes and their rewiring in influenza A virus infection
Zhang J et al. iScience 2024; 27 (8) : 110424 Influenza A virus infection activates STAT3 to enhance SREBP2 expression, cholesterol biosynthesis, and virus replication.
Yu H et al. Cell Mol Life Sci 2024; 81 (1) : 355 Influenza A virus infection activates caspase-8 to enhance innate antiviral immunity by cleaving CYLD and blocking TAK1 and RIG-I deubiquitination.
X Li et al. Emerg Microbes Infect 2024; 13 (1) : 2387439 The PB2 I714S mutation influenced mammalian adaptation of the H3N2 canine influenza virus by interfering with nuclear import efficiency and RNP complex assembly
H Zhang et al. Research Square 2024; Influenza A Virus Employs Nei Endonuclease VIIILike 1 (NEIL1) to Strength vRNP Function and Block IFN-β Production
Show More +
Submit a Reference

REVIEW

Influenza A virus PB2 protein antibody Cat. No. GTX125926

Rating
( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 4.5 based on 2 users reviews)
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Date : Chen-I Hsu submitted on 16-Sep-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Secondary Antibody : GTX213110-01, Undiluted, 25°C, 1Hr
Lane Description :
Left : A549 cells were transfected with pcDNA3-PB2 (WSN) using Lipofectamine3000. The cell was harvested at 24h post-transfection.
Right : A549 cells were infected with Influenza A (WSN) (MOI=0.1) and harvested at 6, 24, 48 hours postinfection.
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Date : Anonymous submitted on 07-Nov-2014
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : Influenza virus-infected 293T cells
Amount used : 20μg
Blocking : 5% skim milk, 25°C, 1Hr
Submit a Review
Application Reference
G Dugied et al. Mol Cell Proteomics 2024;:100856 Profiling Cullin4-E3 ligases interactomes and their rewiring in influenza A virus infection
Zhang J et al. iScience 2024; 27 (8):110424 Influenza A virus infection activates STAT3 to enhance SREBP2 expression, cholesterol biosynthesis, and virus replication.
Yu H et al. Cell Mol Life Sci 2024; 81 (1):355 Influenza A virus infection activates caspase-8 to enhance innate antiviral immunity by cleaving CYLD and blocking TAK1 and RIG-I deubiquitination.
X Li et al. Emerg Microbes Infect 2024; 13 (1):2387439 The PB2 I714S mutation influenced mammalian adaptation of the H3N2 canine influenza virus by interfering with nuclear import efficiency and RNP complex assembly
H Zhang et al. Research Square 2024; Influenza A Virus Employs Nei Endonuclease VIIILike 1 (NEIL1) to Strength vRNP Function and Block IFN-β Production
YJ Li et al. PLoS Pathog 2024; 20 (5):e1012279 OTUB1 contributes to the stability and function of Influenza A virus NS2
Y Sun et al. Research Square 2024; Influenza virus infection activates TAK1 to suppress RIPK3- independent apoptosis and RIPK1-dependent necroptosis
Y Li et al. Phytomed Plus 2024; 4 (2):100553 Cepharanthine inhibits influenza A virus replication by impairing viral polymerase activity and regulating influenza-induced immune response
WK Cho et al. J Funct ?Foods 2024; 112:105940 Hoveniae Semen Seu Fructus water extract inhibits influenza A virus infection
Application : ICC/IF
Sun L et al. Sci Adv 2023; 9 (28):eadg5175 The SUMO-interacting motif in NS2 promotes adaptation of avian influenza virus to mammals
Application : WB
Reactivity : Influenza A virus
Sheppard CM et al. Nat Commun 2023; 14 (1):6135 An Influenza A virus can evolve to use human ANP32E through altering polymerase dimerization.
Yang H et al. PLoS Pathog 2023; 19 (7):e1011489 The synergistic effect of residues 32T and 550L in the PA protein of H5 subtype avian influenza virus contributes to viral pathogenicity in mice.
Peacock TP et al. J Virol 2023; 97 (5):e0021323 Mammalian ANP32A and ANP32B Proteins Drive Differential Polymerase Adaptations in Avian Influenza Virus.
Swann OC et al. mBio 2023; 14 (1):e0339922 Avian Influenza A Virus Polymerase Can Utilize Human ANP32 Proteins To Support cRNA but Not vRNA Synthesis.
Application : ICC/IF
Chen J et al. Virol Sin 2023; 38 (1):128-141 Nonmuscle myosin IIA promotes the internalization of influenza A virus and regulates viral polymerase activity through interacting with nucleoprotein in human pulmonary cells.
Application : WB
G?nl F et al. Nat Commun 2023; 14 (1):787 The ubiquitination landscape of the influenza A virus polymerase.
Application : WB, ICC/IF
Panagiotidis GD et al. Arch Virol 2023; 168 (2):63 Influenza A virus strain PR/8/34, but neither HAM/2009 nor WSN/33, is transiently inhibited by the PB2-targeting drug paliperidone.
Application : WB, IP
Seoane R et al. Front Cell Infect Microbiol 2022; 12:960138 eIF5A is activated by virus infection or dsRNA and facilitates virus replication through modulation of interferon production.
Application : WB
Zheng H et al. J Virol 2022; 96 (12):e0049422 G Protein Subunit β1 Facilitates Influenza A Virus Replication by Promoting the Nuclear Import of PB2.
Application : WB
Yang H et al. Nat Commun 2022; 13 (1):6288 The influenza virus PB2 protein evades antiviral innate immunity by inhibiting JAK1/STAT signalling.
Application : WB, ICC/IF
Huang Y et al. Viruses 2022; 14 (12) Nisoldipine Inhibits Influenza A Virus Infection by Interfering with Virus Internalization Process.
Li Q et al. Adv Sci (Weinh) 2022; 9 (34):e2203088 SRSF5-Mediated Alternative Splicing of M Gene is Essential for Influenza A Virus Replication: A Host-Directed Target Against Influenza Virus.
French H et al. Sci Adv 2022; 8 (36):eabp8655 Transient RNA structures cause aberrant influenza virus replication and innate immune activation.
Guangwen Wang et al. PLoS Pathog 2022; 18 (4):e1010446 PIAS1-mediated SUMOylation of influenza A virus PB2 restricts viral replication and virulence.
Ma C et al. PLoS Pathog 2022; 18 (2):e1010295 p21 restricts influenza A virus by perturbing the viral polymerase complex and upregulating type I interferon signaling.
Application : WB
Xin Mao et al. Evid Based Complement Alternat Med 2021; 2021:5547424 Essential Oil-Rich Chinese Formula Luofushan-Baicao Oil Inhibits the Infection of Influenza A Virus through the Regulation of NF-κB P65 and IRF3 Activation.
Application : WB
Bdeir N et al. Sci Rep 2021; 11 (1):20477 Evidence that two instead of one defective interfering RNA in influenza A virus-derived defective interfering particles (DIPs) does not enhance antiviral activity.
Application : WB
Zhao X et al. Viruses 2021; 13 (8) 3-Indoleacetonitrile Is Highly Effective in Treating Influenza A Virus Infection In Vitro and In Vivo.
Application : WB
Liu X et al. Nat Commun 2021; 12 (1):4427 MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.
Application : WB
Van den Hoecke S et al. mBio 2021; 12 (4):e0074521 In Vivo Therapy with M2e-Specific IgG Selects for an Influenza A Virus Mutant with Delayed Matrix Protein 2 Expression.
Application : WB
Gong W et al. J Virol 2021; Interaction of NEP with G Protein Pathway Suppressor 2 Facilitates Influenza A Virus Replication by Weakening the Inhibition of GPS2 to RNA Synthesis and Ribonucleoprotein Assembly.
Application : WB
Zhu Y et al. Sci Adv 2020; 6 (25):eaaz5764 Human TRA2A determines influenza A virus host adaptation by regulating viral mRNA splicing.
Peacock TP et al. J Virol 2020; 94 (12) Swine ANP32A Supports Avian Influenza Virus Polymerase.
Gao Q et al. J Virol 2020; 95 (2) Eukaryotic Translation Elongation Factor 1 Delta Inhibits the Nuclear Import of the Nucleoprotein and PA-PB1 Heterodimer of Influenza A Virus.
Application : WB
Wang J et al. Viruses 2020; 12 (10) In Vitro and In Vivo Antiviral Activity of Gingerenone A on Influenza A Virus Is Mediated by Targeting Janus Kinase 2.
Application : WB
Szeto WC et al. PLoS One 2020; 15 (9):e0239899 Interaction between influenza A virus nucleoprotein and PB2 cap-binding domain is mediated by RNA.
Application : WB
Carrique L et al. Nature 2020; 587 (7835):638-643 Host ANP32A mediates the assembly of the influenza virus replicase.
Application : WB
Declercq M et al. Nucleic Acids Res 2020; 48 (18):10428-10440 Influenza A virus co-opts ERI1 exonuclease bound to histone mRNA to promote viral transcription.
Application : WB
https://www.biorxiv.org/content/10.1101/2020.04.06.027482v1.abstract et al. bioRxiv 2020; (Epub) A rare variant in Anp32B impairs influenza virus replication in human cells.
Application : WB
Thomas P. Peacock et al. bioRxiv 2020; (Epub) Swine ANP32A supports avian influenza virus polymerase3
Application : WB
Mistry B et al. J Virol 2020; 94 (3) Elucidating the Interactions between Influenza Virus Polymerase and Host Factor ANP32A.
Zhang Q et al. Microbes Infect 2020; (Epub) SNW1 interacts with IKKγ to positively regulate antiviral innate immune responses against influenza A virus infection.
Application : WB
Zhang X et al. Front Microbiol 2020; 11:538355 Avian Chaperonin Containing TCP1 Subunit 5 Supports Influenza A Virus Replication by Interacting With Viral Nucleoprotein, PB1, and PB2 Proteins.
Application : WB
Hu A et al. Front Microbiol 2020; 11:178 Anthralin Suppresses the Proliferation of Influenza Virus by Inhibiting the Cap-Binding and Endonuclease Activity of Viral RNA Polymerase.
Application : WB
Cai M et al. Viruses 2020; 12 (1) The R251K Substitution in Viral Protein PB2 Increases Viral Replication and Pathogenicity of Eurasian Avian-like H1N1 Swine Influenza Viruses.
Application : WB
Monteagudo PL et al. J Virol 2019; Differential modulation of innate immune responses in human primary cells by influenza A viruses carrying human or avian non-structural 1 proteins.
Application : WB
Staller E et al. J Virol 2019; (Epub) ANP32 proteins are essential for influenza virus replication in human cells.
Application : WB
Wang R et al. J Virol 2019; 93 (4) Autophagy Promotes Replication of Influenza A Virus In Vitro.
Chao TL et al. Front Microbiol 2019; 10:2942 Characterization of Influenza A Virus Infection in Mouse Pulmonary Stem/Progenitor Cells.
Application : WB
Bi Z et al. Emerg Microbes Infect 2019; 8 (1):1465-1478 Insights into species-specific regulation of ANP32A on the mammalian-restricted influenza virus polymerase activity.
Long JS et al. Elife 2019; 8 Species specific differences in use of ANP32 proteins by influenza A virus.
Hao X et al. Transbound Emerg Dis 2019; (Epub) The PB2 and M genes are critical for the superiority of genotype S H9N2 virus to genotype H in optimizing viral fitness of H5Nx and H7N9 avian influenza viruses in mice.
Application : WB, ICC/IF
Chen F et al. Pathog Dis 2019; 77 (6) Key amino acid residues of neuraminidase involved in influenza A virus entry.
Application : ICC/IF
Choi JG et al. Sci Rep 2019; 9 (1):12132 Antiviral activity of ethanol extract of Geranii Herba and its components against influenza viruses via neuraminidase inhibition.
Application : WB
Arai Y et al. PLoS Pathog 2019; 15 (7):e1007919 PB2 mutations arising during H9N2 influenza evolution in the Middle East confer enhanced replication and growth in mammals.
Application : WB
Hao X et al. Virology 2019; 535:218-226 The PB2 and M genes of genotype S H9N2 virus contribute to the enhanced fitness of H5Nx and H7N9 avian influenza viruses in chickens.
Application : WB
Long J.S. et al. bioRxiv 2019; (Epub) Avian ANP32B does not support influenza A virus polymerase and influenza A virus relies exclusively on ANP32A in chicken cells.
Application : WB
Li J et al. Biochem J 2019; 476 (3):467-481 MOV10 sequesters the RNP of influenza A virus in the cytoplasm and is antagonized by viral NS1 protein.
Application : WB
Aydillo T et al. J Virol 2018; 92 (7) Specific Mutations in the PB2 Protein of Influenza A Virus Compensate for the Lack of Efficient Interferon Antagonism of the NS1 Protein of Bat Influenza A-Like Viruses.
Te Velthuis AJW et al. Nat Microbiol 2018; 3 (11):1234-1242 Mini viral RNAs act as innate immune agonists during influenza virus infection.
Liu T et al. Mol Pharm 2018; 15 (9):4110-4120 A Small-Molecule Compound Has Anti-influenza A Virus Activity by Acting as a ''PB2 Inhibitor".
Application : WB
Lin HT et al. Sci Rep 2018; 8 (1):17242 Grail attenuates influenza A virus infection and pathogenesis by inhibiting viral nucleoprotein.
Application : WB
Yang C et al. J Virol 2018; (Epub) The nucleolar protein LYAR facilitates ribonucleoprotein assembly of influenza A virus.
Application : WB, ICC/IF
Wang J et al. Viruses 2018; 10 (6) Host Long Noncoding RNA lncRNA-PAAN Regulates the Replication of Influenza A Virus.
Application : WB
Aartjan te Velthuis et al. bioRxiv 2018; (Epub) Mini viral RNAs act as innate immune agonists during influenza virus infection.
Application : WB
Oymans J et al. J Virol 2017; (Epub) A mechanism for prime-realignment during influenza A virus replication.
Application : WB
Oymans J et al. bioRxiv 2017; (Epub) A mechanism for prime-realignment during influenza A virus replication.
Application : WB
Wang L et al. Nat Commun 2017; 8:13876 Comparative influenza protein interactomes identify the role of plakophilin 2 in virus restriction.
Application : WB
Shin D et al. J Virol 2017; (Epub) double phd fingers 2 (dpf2) promotes the immune escape of influenza virus by suppressing *-β production.
Application : ICC/IF
Arai Y et al. PLoS Pathog 2016; 12 (4):e1005583 Novel Polymerase Gene Mutations for Human Adaptation in Clinical Isolates of Avian H5N1 Influenza Viruses.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169