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Influenza B virus Nucleoprotein antibody [GT382]

Anti-Influenza B virus Nucleoprotein antibody [GT382] used in ELISA (ELISA). GTX629883
Anti-Influenza B virus Nucleoprotein antibody [GT382] used in Western Blot (WB). GTX629883
Anti-Influenza B virus Nucleoprotein antibody [GT382] used in ELISA (ELISA). GTX629883
Anti-Influenza B virus Nucleoprotein antibody [GT382] used in ELISA (ELISA). GTX629883
Anti-Influenza B virus Nucleoprotein antibody [GT382] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629883

Cat. No. GTX629883

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT382

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA, Sandwich ELISA

Reactivity

 Influenza B virus
Package
100 μl ($399),
25 μl ($169)
See all Influenza B virus Nucleoprotein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX629883, Detection : GTX128539 / GTX128538
Not tested in other applications.

Positive Control

Influenza B virus (Taiwan 70555) infected MDCK cells

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza B virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Influenza B virus Nucleoprotein (B/Taiwan/753/2005). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888173

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza B Virus Nucleoprotein , Influenza B Virus NP

Research Area

DATA IMAGES

Anti-Influenza B virus Nucleoprotein antibody [GT382] used in ELISA (ELISA). GTX629883

GTX629883 ELISA Image

Sandwich ELISA detection of non-transfected and Influenza B virus (B/Sydney/3/2004) nucleoprotein transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [GT382] (GTX629883) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128539) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza B virus Nucleoprotein antibody [GT382] used in Western Blot (WB). GTX629883

GTX629883 WB Image

Influenza B Virus NP antibody [GT382] detects Influenza B Virus NP protein by Western blot analysis.
A. 10 μg MDCK whole cell lysate/extract
B. 10 μg whole cell lysate/extract of Influenza B virus (Taiwan 70555) infected MDCK cells
7.5 % SDS-PAGE
Influenza B Virus NP antibody [GT382] (GTX629883) dilution: 1:5000

Anti-Influenza B virus Nucleoprotein antibody [GT382] used in ELISA (ELISA). GTX629883

GTX629883 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza B virus NP (nucleoprotein) protein, Flag tag (GTX135867-pro) using Influenza B virus Nucleoprotein antibody [GT382] (GTX629883) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128538) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza B virus Nucleoprotein antibody [GT382] used in ELISA (ELISA). GTX629883

GTX629883 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza B virus NP (nucleoprotein) protein, DDDDK tag (GTX135867-pro) using Influenza B virus Nucleoprotein antibody [GT382] (GTX629883) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128539) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza B virus Nucleoprotein antibody [GT382] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629883

GTX629883 ICC/IF Image

Influenza B Virus NP antibody [GT382] detects Influenza B Virus NP protein by immunofluorescent analysis.
Sample: Influenza B Virus infected MDCK cells were fixed in paraformaldehyde.
Green: Influenza B Virus NP protein stained by Influenza B Virus NP antibody [GT382] (GTX629883) diluted at 1:2000.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations