APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
| Assay dependent |
| 1:100-1:1000 |
| Assay dependent |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat-2
Predict Reactivity
Sheep, Bovine, Cat, Chicken, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.51 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide encompassing a sequence within the Intracellular domain of human Integrin beta 1 / CD29. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885823
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
integrin subunit beta 1 , CD29 , FNRB , GPIIA , MDF2 , MSK12 , VLA-BETA , VLAB
Cellular Localization
Cell membrane,Cleavage furrow,Melanosome
Background
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX128839 WB Image
Wild-type (WT) and Integrin beta 1 / CD29 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:400. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128839 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:10000 and competitor's antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX128839 ICC/IF Image
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cell membrane by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX128839 IP Image
Immunoprecipitation of Integrin beta 1 / CD29 protein from HeLa whole cell extracts using 5 μg of Integrin beta 1 / CD29 antibody (GTX128839).
Western blot analysis was performed using Integrin beta 1 / CD29 antibody (GTX128839).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX128839 IHC-P Image
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein on breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
Integrin beta 1 / CD29 antibody (GTX128839) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX128839 IHC-P Image
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX128839 WB Image
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg BCL-1 whole cell lysate/extract
C. 30 μg Raw264.7 whole cell lysate/extract
D. 30 μg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
Integrin beta 1 / CD29 antibody (GTX128839) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128839 WB Image
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
7.5% SDS-PAGE
Integrin beta 1 / CD29 antibody (GTX128839) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128839 IHC-P Image
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX128839 IHC-P Image
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brown adipocyte.Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX128839 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128839 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128839 WB Image
Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)
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GTX128839 IHC-P Image
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX128839 ICC/IF Image
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cell membrane by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX128839 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128839 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128839 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128839 WB Image
The data was published in the journal Oncotarget in 2017. PMID: 29050309
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REFERENCE
REVIEW
Integrin beta 1 / CD29 antibody
Cat. No. GTX128839
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Rating
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( Average 4.3 based on 3 users reviews)
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Application
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Western Blot(WB)
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( Average 4 based on 1 users reviews)
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IHC (Frozen sections)(IHC-Fr)
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( Average 5 based on 1 users reviews)
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Immunoprecipitation(IP)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 17-Aug-2021
Application Tested :
IHC-Fr
Sample Species :
Ms
Sample :
14dpp C57BL/6J mouse testis
Blocking :
5% donkey-serum in TNB, 25°C, 1Hr
Fixation :
methanol:acetone = 1:1 for 7min
Primary Antibody :
Figure Note :
Peritubular myoid cells (PMCs, arrows; smooth muscle actin (SMA) is a marker) which surround seminiferous tubules (*) express integrin beta 1.
Red : Integrin beta 1 / CD29
Green : SMA
Blue : DAPI
Date :
Anonymous submitted on 18-Jun-2019
Application Tested :
WB
Sample Species :
Hu, Ms
Amount used :
30 μg
Blocking :
5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody :
1:500, 25°C, 1Hr
Lane Description :
Lane 1: mouse endothelial cells; Lane 2: TOV112D; Lane 3: CaCo2; Lane 4: HT29
Date :
Anonymous submitted on 13-Dec-2013
Application Tested :
IP
Sample Species :
Hu
Sample :
J82 cells
Amount used :
500μg
Blocking :
5% BSA, 25°C, 2Hr
Fixation :
NP-40 lysis buffer
Primary Antibody :
1:10000, 4°C, 16Hr
Figure Note :
1 ug for IP
