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6X His tag antibody [GT359]

Comparison
Anti-6X His tag antibody [GT359] used in Immunoprecipitation (IP). GTX628914
Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914
Anti-6X His tag antibody [GT359] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628914
Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914
Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914
Anti-6X His tag antibody [GT359] used in ELISA (ELISA). GTX628914
Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914
Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914
Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914
Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914
Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914
Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914

Cat. No. GTX628914

Reference 45
1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT359

Isotype

 IgG3

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all 6X His tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
IP 1:100-1:500
ELISA 1:1000-1:10000
Not tested in other applications.

Positive Control

His-recombinant protein

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.59 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to 6X His tag

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2750602

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

6X His tag antibody , His antibody , 6x His antibody , 6x his tag antibody , His Tag antibody , 6xHis Tag antibody , 6 x His antibody

Research Area

DATA IMAGES

Anti-6X His tag antibody [GT359] used in Immunoprecipitation (IP). GTX628914

GTX628914 IP Image

6X His tag antibody [GT359] immunoprecipitates His-fused protein in IP experiments.
IP Sample: cell lysate/extract of His-fused gene transfected HeLa cells
A. 1/20 lysate/extract of transfected HeLa cells
B. Control with 2 μg of preimmune mouse IgG
C. Immunoprecipitation of His-fused protein by 2 μg of 6X His tag antibody [GT359] (GTX628914)
12% SDS-PAGE
The immunoprecipitated His-fused protein was detected by 6X His tag antibody [GT359] (GTX628914) diluted at 1:1000.

Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914

GTX628914 WB Image

6x His antibody detects 6x His protein by Western blot analysis.
A. Non-induced cell lysates from E. coli expressing HIS-tagged proteins
B. IPTG-induced cell lysates from E. coli expressing HIS-tagged proteins
15 % SDS-PAGE
6x His antibody (GTX628914) dilution: 1:5000

Anti-6X His tag antibody [GT359] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628914

GTX628914 ICC/IF Image

6X His tag antibody [GT359] detects 6X His tag protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: 6X His tag stained by 6X His tag antibody [GT359] (GTX628914) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914

GTX628914 WB Image

6x His antibody detects 6x His protein by Western blot analysis.
A. 2 μg GST-recombinant protein
B. 2 μg His-recombinant protein
C. 1 μg His-recombinant protein
D. 0.5 μg His-recombinant protein E. 0.1 μg His-recombinant protein
E. 0.1 μg His-recombinant protein E. 0.1 μg His-recombinant protein
12 % SDS-PAGE
6x His antibody (GTX628914) dilution: 1:5000

Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914

GTX628914 WB Image

6x His antibody detects 6x His protein by Western blot analysis.
Lane A and B were IPTG-induced cell lysate from E. coli expressing different GST-tagged proteins.
Lane C to G were IPTG induced cell lysate from E. coli expressing different His-tagged protein.
12 % SDS-PAGE
6x His antibody (GTX628914) dilution: 1:5000

Anti-6X His tag antibody [GT359] used in ELISA (ELISA). GTX628914

GTX628914 ELISA Image

6X His tag antibody [GT359] detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 0.5 μg/ml 6X His tag antibody [GT359] (GTX628914). Bound Abs were further detected by using HRP-conjugated goat anti-mouse IgG (GTX213111-01) with HRP substrates.

Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914

GTX628914 WB Image

Comparison

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with 6X His tag antibody [GT359] (GTX628914) diluted at 1:1000 and competitor's antibody (sc-8036) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914

GTX628914 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6X His tag antibody [GT359] (GTX628914) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914

GTX628914 WB Image

The data was published in the journal Mol Cancer in 2014. PMID: 25362534

Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914

GTX628914 WB Image

The data was published in the journal PLoS One in 2014. PMID: 25285446

Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914

GTX628914 WB Image

The data was published in the journal PLoS One in 2014. PMID: 25285446

Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914

GTX628914 WB Image

The data was published in the journal J Biomed Sci in 2018.PMID: 29361943

REFERENCE

Application Reference

Silamparasan D et al. Front Plant Sci 2023; 14 : 1093472 Calcium-dependent protein kinase CDPK16 phosphorylates serine-856 of glutamate receptor-like GLR3.6 protein leading to salt-responsive root growth in Arabidopsis().
Application : WB
Fang J et al. iScience 2023; 26 (12) : 108551 Scramblase activity of proteorhodopsin confers physiological advantages to Escherichia coli in the absence of light.
Inoue Y et al. Protein Sci 2022; 31 (6) : e4331 Structure-based alteration of tryptophan residues of the multidrug transporter CmABCB1 to assess substrate binding using fluorescence spectroscopy.
Ko SH et al. J Biomed Sci 2022; 29 (1) : 108 Monoclonal antibodies against S2 subunit of spike protein exhibit broad reactivity toward SARS-CoV-2 variants.
Shao-Hsuan Wen et al. Int J Mol Sci 2021; 22 (21) Helicobacter pylori Neutrophil-Activating Protein Directly Interacts with and Activates Toll-like Receptor 2 to Induce the Secretion of Interleukin-8 from Neutrophils and ATRA-Induced Differentiated HL-60 Cells.
Application : ELISA
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REVIEW

6X His tag antibody [GT359] Cat. No. GTX628914

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914
Date : Anonymous submitted on 13-Jun-2014
Score :
Application Tested : WB
Sample : Fission yeast cells transfected His-Tdh1
Amount used : 1μg
Blocking : 3% skim milk in TBS+0.3% tween20, 25°C, 16Hr
Primary Antibody : 1:2000, 25°C, 1Hr
Lane Description : A:Fission yeast cells, B:Fission yeast cells transfected His-Tdh1
Submit a Review
Application Reference
Silamparasan D et al. Front Plant Sci 2023; 14:1093472 Calcium-dependent protein kinase CDPK16 phosphorylates serine-856 of glutamate receptor-like GLR3.6 protein leading to salt-responsive root growth in Arabidopsis().
Application : WB
Fang J et al. iScience 2023; 26 (12):108551 Scramblase activity of proteorhodopsin confers physiological advantages to Escherichia coli in the absence of light.
Inoue Y et al. Protein Sci 2022; 31 (6):e4331 Structure-based alteration of tryptophan residues of the multidrug transporter CmABCB1 to assess substrate binding using fluorescence spectroscopy.
Ko SH et al. J Biomed Sci 2022; 29 (1):108 Monoclonal antibodies against S2 subunit of spike protein exhibit broad reactivity toward SARS-CoV-2 variants.
Sung-Chan Wei et al. Front Immunol 2021; 12:771011 An Integrated Platform for Serological Detection and Vaccination of COVID-19.
Application : WB
Nisha Tapryal et al. J Biol Chem 2021; 296:100723 Intrapulmonary administration of purified NEIL2 abrogates NF-κB-mediated inflammation.
Application : WB
Miao-Hsi Hsieh et al. Front Immunol 2021; 12:641360 Human Surfactant Protein D Binds Spike Protein and Acts as an Entry Inhibitor of SARS-CoV-2 Pseudotyped Viral Particles.
Application : ELISA
Yun-Hsiang Chen et al. Sci Rep 2021; 11 (1):12398 The potential of adoptive transfer of γ9δ2 T cells to enhance blinatumomab's antitumor activity against B-cell malignancy.
Application : WB
Robert John S Lamis et al. Diagnostics (Basel) 2021; 11 (6) Identification and Quantification of Anti-Gp.Mur Antibodies in Human Serum Using an Insect-Cell-Based System.
Application : WB, ELISA
Shao-Hsuan Wen et al. Int J Mol Sci 2021; 22 (21) Helicobacter pylori Neutrophil-Activating Protein Directly Interacts with and Activates Toll-like Receptor 2 to Induce the Secretion of Interleukin-8 from Neutrophils and ATRA-Induced Differentiated HL-60 Cells.
Application : ELISA
Wong CW et al. Molecules 2021; 26 (23) Engineered Bacteriorhodopsin May Induce Lung Cancer Cell Cycle Arrest and Suppress Their Proliferation and Migration.
Application : ICC/IF
Niborski LL et al. EBioMedicine 2021; 63:103206 Recombinant antibody against Trypanosoma cruzi from patients with chronic Chagas heart disease recognizes mammalian nervous system.
Roche B et al. FEBS Lett 2021; 595 (3):370-378 Identification of the fatty acid coenzyme-A ligase FadD1 as an interacting partner of FptX in the Pseudomonas?aeruginosa pyochelin pathway.
Application : WB
Wang Y et al. FASEB J 2019;:fj201801966RRR Macrophage migration inhibitory factor derived from spinal cord is involved in activation of macrophages following gecko tail amputation.
Application : WB
Tsai CH et al. ACS Synth Biol 2019; Generation of Stable Influenza Virus Hemagglutinin through Structure-Guided Recombination.
Application : WB, ICC/IF
Li CH et al. Toxins (Basel) 2019; 11 (5) Recombinant Aflatoxin-Degrading F420H2-Dependent Reductase from Mycobacterium smegmatis Protects Mammalian Cells from Aflatoxin Toxicity.
Application : WB
Yu-Fon Chen et al. Materials Science & Engineering C 2019; 102:85-95 TRAIL encapsulated to polypeptide-crosslinked nanogel exhibits increased anti-inflammatory activities in Klebsiella pneumoniae-induced sepsis treatment.
Application : WB
Itoh K et al. PLoS One 2019; 14 (5):e0216083 Isolation of nanobodies against Xenopus embryonic antigens using immune and non-immune phage display libraries.
Application : WB, ELISA
Wei SC et al. J Virol 2019; (Epub) Baculovirus IE2 interacts with viral DNA through Daxx to generate an organized nuclear body structure for gene activation in Vero cells.
Application : ICC/IF, IP
Hu CM et al. Cell Metab 2019; (Epub) High Glucose Triggers Nucleotide Imbalance through O-GlcNAcylation of Key Enzymes and Induces KRAS Mutation in Pancreatic Cells.
Application : WB
Huang KY et al. J Microbiol Immunol Infect 2018; (Epub) Potential role of autophagy in proteolysis in Trichomonas vaginalis.
Application : WB
Armenta S et al. Data Brief 2018; 21:1944-1949 Data concerning secondary structure and alpha-glucans-binding capacity of the LaCBM26.
Journal of the Taiwan Institute of Chemical Engineers et al. 0 2018; (Epub) Ethanol production from hemicellulose by a consortium of different genetically-modified sacharomyces cerevisiae.
Application : WB
Serrano-Maldonado CE et al. J Mol Microbiol Biotechnol 2018; 28 (1):14-27 Cloning and Characterization of a Novel N-acetylglucosaminidase (AtlD) from Enterococcus faecalis.
Application : WB
Chou YT et al. J Biomed Sci 2018; 25 (1):6 Identification of as an isoform-specific inhibitor of HEME OXYGENASE-1/cytochrome P450 reductase interaction with anti-tumor activity.
Application : WB
Tabanag I.D. et al. 0 2018; (Epub) Hemicellulose Degradation and Utilization by a Synthetic Saccharomyces cerevisiae Consortium.
Application : WB
Huang YJ et al. Nanotheranostics 2017; 1 (3):326-337 TRAIL-functionalized gold nanoparticles selectively trigger apoptosis in polarized macrophages.
Application : WB
Ho CF et al. Arch Virol 2017; (Epub) Development of an antigen-capture ELISA for beak and feather disease virus.
Application : WB
Doud DFR et al. Biotechnol Biofuels 2017; 10:178 Metabolic engineering of Rhodopseudomonas palustris for the obligate reduction of n-butyrate to n-butanol.
Application : WB
Loiseau L et al. J Biol Chem 2017; (Epub) The UbiK protein is an accessory factor necessary for bacterial ubiquinone (UQ) biosynthesis and forms a complex with the UQ biogenesis factor UbiJ.
Application : WB
Erwig J et al. New Phytol 2017; (Epub) Chitin-induced and CHITIN ELICITOR RECEPTOR KINASE1 (CERK1) phosphorylation-dependent endocytosis of Arabidopsis thaliana LYSIN MOTIF-CONTAINING RECEPTOR-LIKE KINASE5 (LYK5).
Application : WB
Su FH et al. Chem. Eng. J. 2017; 308 (15):43107 Decorating outer membrane vesicles with organophosphorus hydrolase and cellulose binding domain for organophosphate pesticide degradation.
Application : WB
Churion KA et al. Anal Biochem 2016; 514:43323 Separating full-length protein from aggregating proteolytic products using filter flow-through purification.
Application : WB
Liao YJ et al. Transfusion 2016; The SHP2-ERK2 signaling pathway regulates branched I antigen formation by controlling the binding of CCAAT/enhancer binding protein α to the IGnTC promoter region during erythroid differentiation.
Application : WB
Wang L et al. Cell Cycle 2015; 14 (1):157-66 Regulation of polo-like kinase 1 by DNA damage and PP2A/B55α.
Application : WB
Kara I et al. J Biol Chem 2015; 290 (5):2812-21 the paired basic amino acid-cleaving enzyme 4 (pace4) is involved in the maturation of * receptor isoform b: an opportunity to reduce the specific * receptor-dependent effects of *-like growth factor 2 (igf2).
Application : WB
Hong JB et al. PLoS One 2014; 9 (2):e89396 A nucleolus-predominant piggyBac transposase, NP-mPB, mediates elevated transposition efficiency in mammalian cells.
Application : WB
Deng W et al. Endocrinology 2014; 155 (5):1618-28 Protein kinase B (PKB/AKT1) formed signaling complexes with mitochondrial proteins and prevented glycolytic energy dysfunction in cultured cardiomyocytes during ischemia-reperfusion injury.
Itoh K et al. PLoS One 2014; 9 (10):e107521 Expression cloning of camelid nanobodies specific for Xenopus embryonic antigens.
Application : WB
Zhuang H et al. Mol Cancer 2014; 13:243 Human epididymis protein 4 in association with Annexin II promotes invasion and metastasis of ovarian cancer cells.
Application : WB
Watashi K et al. Hepatology 2014; 59 (5):1726-37 Cyclosporin A and its analogs inhibit hepatitis B virus entry into cultured hepatocytes through targeting a membrane transporter NTCP.
Application : ELISA
Clone Reference
Teruyo Ojima-Kato et al. Antibodies 2018; 7 (4):38 Rapid Generation of Monoclonal Antibodies from Single B Cells by Ecobody Technology.
Kowalczyk T et al. AMB Express 2018; 8 (1):125 High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes.
Ng WK et al. Biotechnol Appl Biochem 2017; (Epub) Human neonatal Fc receptor as a new potential antibody binding protein for antibody immobilization.
Lin CC et al. Acta Crystallogr D Biol Crystallogr 2015; 71 (Pt 4):1006-21 Structures of the hydrolase domain of zebrafish 10-formyltetrahydrofolate dehydrogenase and its complexes reveal a complete set of key residues for hydrolysis and product inhibition.
SDS
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$ 399
$ 169