GeneTex

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United States (US)

Antigen Retrieval Solution

Cat. No. GTX20970

Application

 IHC-P
Package
1 kit ($259)
See all Antigen Retrieval Reagent products
PRODUCT

Summary

Antigen retrieval solution-enzymatic (trypsin)

APPLICATION

Application Note

Deparaffinize tissues and hydrate to water. If necessary perform hydrogen peroxide block, was in water and rinse in PBS or TBS. The tissue section is now ready for trypsin digestion. Combine 1 part trypsin with 1 part buffer. Incubate for 5-10 miinutes at room temperature. Rinse well in PBS or TBS buffer and proceed with immunostaining procedure.

This product utilises - color-coded buffer (rose) for easy identification. The buffer incorporates a color-coded pH indicator. The end-user can visually inspect the solution and see that the mixture or buffer is at the proper pH. If the mixed solution is purple, the pH is too high for optimum digestion. If the buffer or trypsin solution turns orange or yellow, the pH is too low for optimum digestion. This product is provided as a two component, ready-to-use solution, and can be used at different concentrations: (1) 25 ml Concentrated liquid trypsin (2) 25 ml of trypsin buffer

Positive Control

Infected lung

PROPERTIES

Form

Liquid

Storage

Store concentrated solution at 4ºC. (Stable for 12 months) The working solution is stable for 3 days at 4ºC.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Antigen retrieval solution - enzymatic ( trypsin )

Background

Trypsin is a commonly used digestive enzyme. In formalin-fixed paraffin-embedded tissues, certain antibody protocols required enzyme pretreatment for proper immunohistochemical staining. This product used to be called ""Carezyme Trypsin Enzymatic Antigen Retrieval Solution"".

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Package List Price ($)
$ 259