APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Molt-4 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , U87-MG , SK-N-SH , IMR-32 , SK-N-AS , rat brain , human Calcineurin A-transfected 293T
Predict Reactivity
Zebrafish, Rabbit, Bovine, Dog, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.98 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Calcineurin A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10721697
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein phosphatase 3 catalytic subunit alpha , ACCIID , CALN , CALNA , CALNA1 , CCN1 , CNA1 , IECEE , IECEE1 , PPP2B
Cellular Localization
Cytoplasm , Cell membrane , Cytoplasm, myofibril, sarcomere, Z line , Cell projection, dendritic spine
Database
Research Area
DATA IMAGES
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GTX111039 ICC/IF Image
Calcineurin A antibody detects Calcineurin A protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Calcineurin A stained by Calcineurin A antibody (GTX111039) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX111039 WB Image
Calcineurin A antibody detects Calcineurin A protein by western blot analysis. Non-transfected (-) and Calcineurin A-transfected (+, ) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A antibody (GTX111039) at a dilution of 1:5000.
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GTX111039 WB Image
Calcineurin A antibody detects Calcineurin A protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A antibody (GTX111039) diluted by 1:3000.
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GTX111039 WB Image
Calcineurin A antibody detects Calcineurin A protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with Calcineurin A antibody (GTX111039) diluted by 1:3000.
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GTX111039 WB Image
Calcineurin A antibody detects Calcineurin A protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A antibody (GTX111039) diluted by 1:3000.
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GTX111039 WB Image
Calcineurin A antibody detects Calcineurin A protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with Calcineurin A antibody (GTX111039) diluted by 1:3000
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GTX111039 WB Image
The data was published in the journal Front Cell Neurosci in 2014. PMID: 25404896
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REFERENCE
REVIEW
Calcineurin A antibody
Cat. No. GTX111039
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Rating
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( Average 5 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 03-Aug-2022
Application Tested :
WB
Sample Species :
Ms
Sample :
JAWS II cells
Amount used :
20μg
Blocking :
3% BSA in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 18Hr
