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Cdh1 antibody

Anti-Cdh1 antibody used in IHC (Whole mount) (IHC-Wm). GTX125890
Anti-Cdh1 antibody used in Immunohistochemistry (IHC). GTX125890

Cat. No. GTX125890

Reference 15

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-Fr, IHC-Wm, IHC

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Cdh1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-Fr Assay dependent
IHC-Wm 1:50-1:500
IHC 1:100-1:1000
Not tested in other applications.

Predict Reactivity

Human (100%)| Mouse (80%)| Rat (83%)| Bovine (82%)| Dog (80%)| Pig (83%)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Cdh1

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167551

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cadherin 1, type 1, E-cadherin (epithelial) , E-cadherin

Database

Research Area

DATA IMAGES

Anti-Cdh1 antibody used in IHC (Whole mount) (IHC-Wm). GTX125890

GTX125890 IHC-Wm Image

Cdh1 antibody detects Cdh1 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Cdh1 antibody (GTX125890) dilution: 1:50.
Anti-Cdh1 antibody used in Immunohistochemistry (IHC). GTX125890

GTX125890 IHC Image

Immunohistochemical analysis of agarose-embedded zebrafish embryo, using E-cadherin (GTX125890) antibody at 1:100 dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)

REFERENCE

Application Reference

Lee JG et al. Microbiome 2022; 10 (1) : 3 Host tp53 mutation induces gut dysbiosis eliciting inflammation through disturbed sialic acid metabolism.
Application : IHC-Fr
Reactivity : Zebrafish
Klatt Shaw D et al. Dev Cell 2021; 56 (5) : 613-626.e7 Localized EMT reprograms glial progenitors to promote spinal cord repair.
Application : IHC-Fr
Reactivity : Zebrafish
Liu R et al. Cell Transplant 2020; 29 : 963689720969167 Characteristics of TGFBR1-EGFR-CTNNB1-CDH1 Signaling Axis in Wnt-Regulated Invasion and Migration in Lung Cancer.
Liu J et al. PLoS Biol 2019; 17 (8) : e3000203 Chemokine signaling links cell-cycle progression and cilia formation for left-right symmetry breaking.
Application : IHC-Wm
Reactivity : Zebrafish
Paul CD et al. Cell Syst 2019; 9 (2) : 187-206.e16 Tissue Architectural Cues Drive Organ Targeting of Tumor Cells in Zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
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REVIEW

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Application Reference
Lee JG et al. Microbiome 2022; 10 (1):3 Host tp53 mutation induces gut dysbiosis eliciting inflammation through disturbed sialic acid metabolism.
Application : IHC-Fr
Reactivity : Zebrafish
Klatt Shaw D et al. Dev Cell 2021; 56 (5):613-626.e7 Localized EMT reprograms glial progenitors to promote spinal cord repair.
Application : IHC-Fr
Reactivity : Zebrafish
Liu R et al. Cell Transplant 2020; 29:963689720969167 Characteristics of TGFBR1-EGFR-CTNNB1-CDH1 Signaling Axis in Wnt-Regulated Invasion and Migration in Lung Cancer.
Liu J et al. PLoS Biol 2019; 17 (8):e3000203 Chemokine signaling links cell-cycle progression and cilia formation for left-right symmetry breaking.
Application : IHC-Wm
Reactivity : Zebrafish
Paul CD et al. Cell Syst 2019; 9 (2):187-206.e16 Tissue Architectural Cues Drive Organ Targeting of Tumor Cells in Zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Enya S et al. Dis Model Mech 2018; 11 (8) A novel zebrafish intestinal tumor model reveals a role for cyp7a1-dependent tumor-liver crosstalk in causing adverse effects on the host.
Hehr CL et al. Cell Adh Migr 2018; 12 (5):489-502 Polarity and morphogenesis of the eye epithelium requires the adhesion junction associated adaptor protein Traf4.
Warga RM et al. Genetics 2018; 210 (4):1391-1409 Probing Cadherin Interactions in Zebrafish with E- and N-Cadherin Missense Mutants.
Application : IHC-Wm
Reactivity : Zebrafish
Mochizuki T et al. Development 2018; 145 (20) Endocytic trafficking factor VPS45 is essential for spatial regulation of lens fiber differentiation in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Franco J.J. et al. bioRxiv 2018; (Epub) Cellular Crowding Influences Extrusion and Proliferation to Facilitate Epithelial Tissue Repair.
Application : IHC-Wm
Reactivity : Zebrafish
Enya S et al. bioRxiv 2017; (Epub) A novel zebrafish intestinal tumor model reveals a role for cyp7a1-dependent tumor-liver crosstalk in tumor's adverse effects on host.
Application : IHC-Fr
Reactivity : Zebrafish
Jimenez L et al. Dis Model Mech 2016; 9 (4):389-400 Phenotypic chemical screening using a zebrafish neural crest EMT reporter identifies retinoic acid as an inhibitor of epithelial morphogenesis.
Application : IHC-Wm
Reactivity : Zebrafish
Ma WR et al. Development 2015; 142 (4):763-73 Jag1b is essential for patterning inner ear sensory cristae by regulating anterior morphogenetic tissue separation and preventing posterior cell death.
Application : IHC-Wm
Reactivity : Zebrafish
Chou CW et al. PLoS One 2014; 9 (9):e107997 The hemodynamically-regulated vascular microenvironment promotes migration of the steroidogenic tissue during its interaction with chromaffin cells in the zebrafish embryo.
Application : IHC-Wm
Reactivity : Zebrafish
Xiong F et al. Cell 2014; 159 (2):415-27 Interplay of cell shape and division orientation promotes robust morphogenesis of developing epithelia.
Application : IHC-Wm
Reactivity : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169