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Cyclin D1 antibody [N1C3]

KO/KD Comparison Orthogonal IP/MS
Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624
Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624
Anti-Cyclin D1 antibody [N1C3] used in ELISA (ELISA). GTX108624
Anti-Cyclin D1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108624
Anti-Cyclin D1 antibody [N1C3] used in Immunoprecipitation (IP). GTX108624
Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624
Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624
Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624
Anti-Cyclin D1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX108624
Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624
Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624

Cat. No. GTX108624

Reference 31
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ELISA, Sandwich ELISA

Reactivity

 Human, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Cyclin D1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP 1:100-1:500
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX634347, Detection: GTX108624
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Product Note

IP/MS validation was supported by references (PMID:30377392)

Predict Reactivity

Mouse, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Cyclin D1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729040

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin D1 , BCL1 , D11S287E , PRAD1 , U21B31

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624

GTX108624 WB Image

KO/KD

Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624

GTX108624 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin D1 antibody [N1C3] used in ELISA (ELISA). GTX108624

GTX108624 ELISA Image

Sandwich ELISA detection of recombinant full-length Cyclin D1 protein using GTX634347 as capture antibody at concentration of 5 μg/mL and GTX108624 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Cyclin D1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108624

GTX108624 ICC/IF Image

Cyclin D1 antibody [N1C3] detects Cyclin D1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cyclin D1 protein stained by Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Scale bar = 10 μm.

Anti-Cyclin D1 antibody [N1C3] used in Immunoprecipitation (IP). GTX108624

GTX108624 IP Image

Immunoprecipitation of Cyclin D1 protein from MCF-7 whole cell extracts using 5 μg of Cyclin D1 antibody [N1C3] (GTX108624).
Western blot analysis was performed using Cyclin D1 antibody [N1C3] (GTX108624).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624

GTX108624 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624

GTX108624 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624

GTX108624 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody (GTX108624) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Cyclin D1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX108624

GTX108624 IHC-P Image

Cyclin D1 antibody detects Cyclin D1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat liver.
Cyclin D1 stained by Cyclin D1 antibody (GTX108624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624

GTX108624 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody (GTX108624) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624

GTX108624 WB Image

Orthogonal

The data was published in the journal J Cell Mol Med in 2020. PMID: 31821701

REFERENCE

Application Reference

Lee SC et al. Pharmaceuticals (Basel) 2023; 16 (9) Synergistic Effect of Ginsenoside Rh2 Combines with Ionizing Radiation on CT26/luc Colon Carcinoma Cells and Tumor-Bearing Animal Model.
Zhang Y et al. Cell Death Discov 2023; 9 (1) : 82
Application : WB
Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Thomas FM et al. Asian Pac J Cancer Prev 2022; 23 (8) : 2863-2871 Modulation of POPDC1 Expression by Phenothiazine and Trifluoperazine Suppress Colon Cancer Growth and Migration.
Application : WB
Reactivity : Human
Yu-Hsuan Hung et al. Int J Mol Sci 2022; 23 (5) Semaphorin 6C Suppresses Proliferation of Pancreatic Cancer Cells via Inhibition of the AKT/GSK3/β-Catenin/Cyclin D1 Pathway.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

Cyclin D1 antibody [N1C3] Cat. No. GTX108624

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 3 users reviews)
Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 0-30 μM DOX treatment for 2 h.
Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624
Date : Anonymous submitted on 21-Nov-2013
Score :
Application Tested : WB
Sample : H184B5F5/M10,BT-474,HCC1937,Hs578T,MCF-7,MDA-MB-231,MDA-MB-361,MDA-MB-453,T47D,ZR-75-1 cells
Amount used : 20μg
Blocking : 1% milk in PBST, 25°C, 0.5Hr
Primary Antibody : 1:5000, 25°C, 2Hr
Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624
Date : Anonymous submitted on 22-Dec-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 20μg
Blocking : 5% non-fat milk in TBST, 4°C, overnightHr
Submit a Review
Application Reference
Lee SC et al. Pharmaceuticals (Basel) 2023; 16 (9) Synergistic Effect of Ginsenoside Rh2 Combines with Ionizing Radiation on CT26/luc Colon Carcinoma Cells and Tumor-Bearing Animal Model.
Zhang Y et al. Cell Death Discov 2023; 9 (1):82
Application : WB
Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Thomas FM et al. Asian Pac J Cancer Prev 2022; 23 (8):2863-2871 Modulation of POPDC1 Expression by Phenothiazine and Trifluoperazine Suppress Colon Cancer Growth and Migration.
Application : WB
Reactivity : Human
Suhaini Sudi et al. Pharmaceuticals (Basel) 2022; 15 (2) Carpaine Promotes Proliferation and Repair of H9c2 Cardiomyocytes after Oxidative Insults.
Application : WB
Reactivity : Rat
Yu-Hsuan Hung et al. Int J Mol Sci 2022; 23 (5) Semaphorin 6C Suppresses Proliferation of Pancreatic Cancer Cells via Inhibition of the AKT/GSK3/β-Catenin/Cyclin D1 Pathway.
Application : WB
Reactivity : Human
Yeu-Sheng Tyan et al. Biosci Rep 2021; 41 (5) Effects of orlistat combined with enzalutamide and castration through inhibition of fatty acid synthase in a PC3 tumor-bearing mouse model.
Application : WB
Reactivity : Human
Huang CY et al. Life (Basel) 2021; 11 (9) Calcitriol Suppresses Warburg Effect and Cell Growth in Human Colorectal Cancer Cells.
Application : WB
Reactivity : Human
Lee W.J. et al. JournalJournal of Food and Drug Analysis 2021; 29 (1):113-127 Tea polyphenol epigallocatechin-3-gallate inhibits cell proliferation in a patient-derived triple-negative breast cancer xenograft mouse model via inhibition of proline-dehydrogenase-induced effects.
Hueng-Chuen Fan et al. Int J Mol Sci 2020; 21 (22) Dehydroxyhispolon Methyl Ether, A Hispolon Derivative, Inhibits WNT/β-Catenin Signaling to Elicit Human Colorectal Carcinoma Cell Apoptosis.
Application : WB
Reactivity : Human
Li M et al. Cancer Manag Res 2020; 12:9159-9171
Application : WB
Reactivity : Human
Huang CW et al. FASEB J 2020; 34 (7):9678-9693 Irisin, an exercise myokine, potently suppresses tumor proliferation, invasion, and growth in glioma.
Application : WB
Reactivity : Human
Wen N et al. J Int Med Res 2020; 48 (1):300060519895089 Overexpression of cell-cycle related and expression-elevated protein in tumor (CREPT) in malignant cervical cancer.
Application : WB
Reactivity : Human
Liang S et al. Oncol Lett 2020; 19 (4):3197-3204 METTL3 serves an oncogenic role in human ovarian cancer cells partially via the AKT signaling pathway.
Application : WB
Reactivity : Human
Huang SW et al. J Cell Mol Med 2020; 24 (2):1822-1836 Lovastatin-mediated MCF-7 cancer cell death involves LKB1-AMPK-p38MAPK-p53-survivin signalling cascade.
Application : WB
Reactivity : Human
Chen KC et al. PLoS One 2019; 14 (12):e0225913 IGF-1-enhanced miR-513a-5p signaling desensitizes glioma cells to?temozolomide?by targeting the NEDD4L-inhibited Wnt/帣-catenin?pathway.
Application : WB
Reactivity : Human
Wu TH et al. Int J Mol Sci 2019; 20 (23)
Application : WB
Reactivity : Human
Wu HY et al. Cancer Lett 2019; 456:13-22 p21-Activated kinase 3 promotes cancer stem cell phenotypes through activating the Akt-GSK3β-β-catenin signaling pathway in pancreatic cancer cells.
Application : WB
Reactivity : Human
Sikorski K et al. Nat Methods 2018; 15 (11):909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Lu WJ et al. Food Funct 2018; 9 (8):4500-4507 Licochalcone A attenuates glioma cell growth in vitro and in vivo through cell cycle arrest.
Application : WB
Reactivity : Human
Wu Z et al. Eur Rev Med Pharmacol Sci 2017; 21 (10):2338-2345 Up-regulation of miR-124 inhibits invasion and proliferation of prostate cancer cells through mediating JAK-STAT3 signaling pathway.
Application : WB
Reactivity : Human
Lin HC et al. J Agric Food Chem 2017; 65 (1):51-59 Antroquinonol, a Ubiquinone Derivative from the Mushroom Antrodia camphorata, Inhibits Colon Cancer Stem Cell-like Properties: Insights into the Molecular Mechanism and Inhibitory Targets.
Application : WB
Reactivity : Human
Berthe A et al. Cell Cycle 2016; 15 (24):3402-3412 Δ2-Troglitazone promotes cytostatic rather than pro-apoptotic effects in breast cancer cells cultured in high serum conditions.
Application : WB
Reactivity : Human
Chen PH et al. Neuropharmacology 2016; (Epub) the chac1-inhibited notch3 pathway is involved in *-induced glioma cytotoxicity.
Application : WB
Reactivity : Human
Chen CC et al. PLoS One 2016; 11 (12):e0168092 Viscolin Inhibits In Vitro Smooth Muscle Cell Proliferation and Migration and Neointimal Hyperplasia In Vivo.
Application : WB
Reactivity : Human
Lu CH et al. Pharmacol Res 2016; 115:288-298 Honokiol, a potential therapeutic agent, induces cell cycle arrest and program cell death in vitro and in vivo in human thyroid cancer cells.
Application : WB
Reactivity : Human
Yen CS et al. Sci Rep 2016; 6:25082 Lovastatin causes FaDu hypopharyngeal carcinoma cell death via AMPK-p63-survivin signaling cascade.
Application : WB
Reactivity : Human
Yeh YH et al. Oncol Rep. 2016; 35 Rhapontigenin inhibits TGF-β-mediated epithelial-mesenchymal transition via the PI3K/AKT/mTOR pathway and is not associated with HIF-1α degradation.
Application : WB
Reactivity : Human
Huang YH et al. Sci Rep 2015; 5:15900 The effects of a novel aliphatic-chain hydroxamate derivative WMJ-S-001 in HCT116 colorectal cancer cell death.
Application : WB
Reactivity : Human
Tsai TH et al. PLoS One 2014; 9 (4):e95443 Pigment Epithelium-Derived Factor 34-mer Peptide Prevents Liver Fibrosis and Hepatic Stellate Cell Activation through Down-Regulation of the PDGF Receptor.
Application : WB
Reactivity : Rat
Lee WY et al. Toxicol Appl Pharmacol 2014; 274 (1):55-62 The induction of heme oxygenase-1 suppresses heat shock protein 90 and the proliferation of human breast cancer cells through its byproduct carbon monoxide.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169