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Dengue virus NS4B protein antibody

Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124250
Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124250
Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124250
Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX124250
Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX124250
Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX124250
Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX124250
Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX124250
Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX124250

Cat. No. GTX124250

Reference 21

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Dengue virus 2
Package
100 μl ($399),
25 μl ($169)
See all Dengue virus NS4B protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
FACS Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

Dengue virus type 2 infected BHK-21 cells , Dengue virus type 3 infected BHK-21 cells , Dengue virus 2-infected C6/36

Product Note

This antibody was raised against Dengue virus 2 NS4B protein, which may cross react with NS4B protein of Dengue virus 3 but does not recognize with NS4B protein of JEV.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Full length Dengue virus 2 NS4B Recombinant protein (Dengue virus 2 (strain 16681 PDK 53))

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176998

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Dengue Virus Non-structural protein 4B , Dengue Virus NS4B , DENV Non-structural protein 4B , DENV NS4B

Research Area

DATA IMAGES

Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124250

GTX124250 ICC/IF Image

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX124250) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124250

GTX124250 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS4B (Dengue virus 2)(GTX124250) antibody at 1:2000 dilution.

Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124250

GTX124250 ICC/IF Image

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX124250) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX124250

GTX124250 WB Image

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
10% SDS PAGE
GTX124250 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX124250

GTX124250 WB Image

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
12% SDS-PAGE
NS4B (Dengue virus) antibody (GTX124250) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX124250

GTX124250 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS4B protein antibody (GTX124250) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX124250

GTX124250 WB Image

The data was published in the journal PLoS Negl Trop Dis in 2014. PMID: 25412261

Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX124250

GTX124250 WB Image

The data was published in the journal PLoS Negl Trop Dis in 2014. PMID: 25412261

Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX124250

GTX124250 WB Image

The data was published in the journal PLoS Negl Trop Dis in 2014. PMID: 25412261

REFERENCE

Application Reference

Shwetha Shivaprasad et al. PLoS Pathog 2022; 18 (4) : e1010163 Loquacious modulates flaviviral RNA replication in mosquito cells.
Application : WB
Mazeaud C et al. Viruses 2021; 13 (10) The Biogenesis of Dengue Virus Replication Organelles Requires the ATPase Activity of Valosin-Containing Protein.
Application : WB
Sarah Goellner et al. STAR Protoc 2020; 1 (3) : 100173 Replication-Independent Generation and Morphological Analysis of Flavivirus Replication Organelles.
Application : WB, ICC/IF
Loe MWC et al. Antiviral Res 2020; 184 : 104954 Betulinic acid exhibits antiviral effects against dengue virus infection.
Application : WB
Cerikan B et al. Cell Rep 2020; 32 (1) : 107859 A Non-Replicative Role of the 3' Terminal Sequence of the Dengue Virus Genome in Membranous Replication Organelle Formation.
Application : ICC/IF
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REVIEW

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Application Reference
Shwetha Shivaprasad et al. PLoS Pathog 2022; 18 (4):e1010163 Loquacious modulates flaviviral RNA replication in mosquito cells.
Application : WB
Mazeaud C et al. Viruses 2021; 13 (10) The Biogenesis of Dengue Virus Replication Organelles Requires the ATPase Activity of Valosin-Containing Protein.
Application : WB
Sarah Goellner et al. STAR Protoc 2020; 1 (3):100173 Replication-Independent Generation and Morphological Analysis of Flavivirus Replication Organelles.
Application : WB, ICC/IF
Loe MWC et al. Antiviral Res 2020; 184:104954 Betulinic acid exhibits antiviral effects against dengue virus infection.
Application : WB
Cerikan B et al. Cell Rep 2020; 32 (1):107859 A Non-Replicative Role of the 3' Terminal Sequence of the Dengue Virus Genome in Membranous Replication Organelle Formation.
Application : ICC/IF
Mohamed B et al. Sci Rep 2020; 10 (1):4040 Very-long-chain fatty acid metabolic capacity of 17-beta-hydroxysteroid dehydrogenase type 12 (HSD17B12) promotes replication of hepatitis C virus and related flaviviruses.
Application : WB
Ngo AM et al. Elife 2019; 8 The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins.
Application : WB
Lee JK et al. Antiviral Res 2019; 171:104589 Antiviral activity of ST081006 against the dengue virus.
Application : WB
P?aszczyca A et al. PLoS Pathog 2019; 15 (5):e1007736 A novel interaction between dengue virus nonstructural protein 1 and the NS4A-2K-4B precursor is required for viral RNA replication but not for formation of the membranous replication organelle.
Wesley Freppel et al. Bio Protoc 2018; 8 (24):e3115 Production, Titration and Imaging of Zika Virus in Mammalian Cells.
Scaturro P et al. Nature 2018; 561 (7722):253-257 An orthogonal proteomic survey uncovers novel Zika virus host factors.
Application : WB
Reid DW et al. J Virol 2018; (Epub) Dengue virus selectively annexes endoplasmic reticulum-associated translation machinery as a strategy for co-opting host cell protein synthesis.
Application : WB
Roth H et al. MBio 2017; 8 (1) Flavivirus Infection Uncouples Translation Suppression from Cellular Stress Responses.
Application : ICC/IF
Hsieh MS et al. PLoS One 2017; 12 (11):e0188170 Detection and quantification of dengue virus using a novel biosensor system based on dengue NS3 protease activity.
Application : ICC/IF
Sepulveda-Salinas KJ et al. Cell Stress Chaperones 2017; (Epub) Participation of dengue virus NS4B protein in the modulation of immune effectors dependent on ER stress in insect cells.
Application : WB
Tsai TT et al. Antiviral Res 2017; 145:44-53 Targeting heat shock factor 1 as an antiviral strategy against dengue virus replication in-vitro and in-vivo.
Application : WB
Ho MR et al. Sci Rep 2017; 7 (1):6910 Blockade of dengue virus infection and viral cytotoxicity in neuronal cells in vitro and in vivo by targeting endocytic pathways.
Application : WB
Wu YW et al. Sci Rep 2016; 6:32243 Autophagy-associated dengue vesicles promote viral transmission avoiding antibody neutralization.
Application : FACS
Fang YT et al. PLoS One 2014; 9 (10):e110655 Autophagy facilitates antibody-enhanced dengue virus infection in human pre-basophil/mast cells.
Application : FACS
Tsai TT et al. PLoS Negl Trop Dis 2014; 8 (11):e3320 Antibody-dependent enhancement infection facilitates dengue virus-regulated signaling of IL-10 production in monocytes.
Application : WB
Poh MK et al. Antiviral Res 2012; 93 (1):191-8 U18666A, an intra-cellular cholesterol transport inhibitor, inhibits dengue virus entry and replication.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169