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Dengue virus Type 1, 2, 3 & 4 antibody [D1-11(3)]

Anti-Dengue virus Type 1, 2, 3 & 4 antibody [D1-11(3)] used in ELISA (ELISA). GTX29202
Anti-Dengue virus Type 1, 2, 3 & 4 antibody [D1-11(3)] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29202

Cat. No. GTX29202

Reference 19
1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 D1-11(3)

Isotype

 IgG2a

Application

 WB, ICC/IF, ELISA

Reactivity

 Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4
Package
250 μg ($399)
See all Dengue virus products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 10 - 2 ug/ml
ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus

Immunogen

Mice were immunized with a mixture of dengue 1, 2, 3 and 4. Spleen cells were used to prepare hybridomas and clones selected based on the ability of the antibody they produced to react with each dengue serotype.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_379737

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Dengue Virus , DENV

Research Area

DATA IMAGES

Anti-Dengue virus Type 1, 2, 3 & 4 antibody [D1-11(3)] used in ELISA (ELISA). GTX29202

GTX29202 ELISA Image

ELISA analysis of immunogen using GTX29202 Dengue virus Type 1, 2, 3 & 4 antibody [D1-11(3)].

Anti-Dengue virus Type 1, 2, 3 & 4 antibody [D1-11(3)] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29202

GTX29202 ICC/IF Image

The data was published in the journal Virol J in 2013. PMID: 23360780

REFERENCE

Application Reference

Afroz S et al. Front Immunol 2020; 11 : 1831 Dengue Virus Induced COX-2 Signaling Is Regulated Through Nutrient Sensor GCN2.
Application : ICC/IF
Dwivedi VD et al. J Biomol Struct Dyn 2020; : 1-14 Anti-dengue infectivity evaluation of bioflavonoid from Azadirachta indica by dengue virus serine protease inhibition.
Application : ICC/IF
Arias-Arias JL et al. Am J Trop Med Hyg 2018; 99 (6) : 1451-1457 Dengue Virus Infection of Primary Human Smooth Muscle Cells.
Application : ICC/IF
Fujiki J et al. Jpn J Infect Dis 2018; (Epub) Single amino acid mutation in dengue virus NS4B protein has opposing effects on viral proliferation in mammalian and mosquito cells.
Application : ICC/IF
Dhanoa A et al. Infect Dis Poverty. 2018; 7 (1) : 1 Seroprevalence of dengue among healthy adults in a rural community in Southern Malaysia: a pilot study.
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REVIEW

Dengue virus Type 1, 2, 3 & 4 antibody [D1-11(3)] Cat. No. GTX29202

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-Dengue virus Type 1, 2, 3 & 4 antibody [D1-11(3)] used in Western Blot (WB). GTX29202
Date : Anonymous submitted on 22-Jul-2014
Score :
Application Tested : WB
Sample Species : DENV1, DENV2, DENV3, DENV4
Sample : DV infected vero cells
Amount used : 1x10^6 cells
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Figure Note : starreivew courtesy of Guey-Chuen Perng, Ph.D. (Laboratory of National Cheng Kung University)
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Application Reference
Afroz S et al. Front Immunol 2020; 11:1831 Dengue Virus Induced COX-2 Signaling Is Regulated Through Nutrient Sensor GCN2.
Application : ICC/IF
Dwivedi VD et al. J Biomol Struct Dyn 2020;:1-14 Anti-dengue infectivity evaluation of bioflavonoid from Azadirachta indica by dengue virus serine protease inhibition.
Application : ICC/IF
Arias-Arias JL et al. Am J Trop Med Hyg 2018; 99 (6):1451-1457 Dengue Virus Infection of Primary Human Smooth Muscle Cells.
Application : ICC/IF
Fujiki J et al. Jpn J Infect Dis 2018; (Epub) Single amino acid mutation in dengue virus NS4B protein has opposing effects on viral proliferation in mammalian and mosquito cells.
Application : ICC/IF
Dhanoa A et al. Infect Dis Poverty. 2018; 7 (1):1 Seroprevalence of dengue among healthy adults in a rural community in Southern Malaysia: a pilot study.
Basso CR et al. Virology 2017; 513:85-90 An easy way to detect dengue virus using nanoparticle-antibody conjugates.
Vicente-Santos A et al. PLoS Negl Trop Dis 2017; 11 (5):e0005537 Neotropical bats that co-habit with humans function as dead-end hosts for dengue virus.
Afroz S et al. Sci Rep 2016; 6:33752 Transcriptome meta-analysis reveals a dysregulation in extra cellular matrix and cell junction associated gene signatures during Dengue virus infection.
Application : ICC/IF
Pitcher TJ et al. J Gen Virol 2015; 96 (Pt 2):288-293 Functional analysis of dengue virus (DENV) type 2 envelope protein domain 3 type-specific and DENV complex-reactive critical epitope residues.
Harahap-Carrillo IS et al. Vaccines (Basel) 2015; 3 (3):503-18 Immunogenic Subviral Particles Displaying Domain III of Dengue 2 Envelope Protein Vectored by Measles Virus.
Application : WB
Liu B et al. J Virol 2014; 88 (23):13821-35 Overlapping and Distinct Molecular Determinants Dictating the Antiviral Activities of TRIM56 against Flaviviruses and Coronavirus.
Application : WB
Manakkadan A et al. Virol J 2013; 10:37 Lineage shift in Indian strains of Dengue virus serotype-3 (Genotype III), evidenced by detection of lineage IV strains in clinical cases from Kerala.
Application : ICC/IF
Barratt-Boyes SM et al. Comp Immunol Microbiol Infect Dis 2012; 35 (3):217-8 Comparative immunology, microbiology and infectious diseases. Introduction.
Application : ICC/IF
Bowen DM et al. Vaccine 2012; 30 (42):1527263 Simplifying complex sequence information: a PCP-consensus protein binds antibodies against all four Dengue serotypes.
Application : ELISA
Pitcher TJ et al. Virology 2012; 422 (2):386-92 Conservation of the DENV-2 type-specific and DEN complex-reactive antigenic sites among DENV-2 genotypes.
Application : ELISA
Gromowski GD et al. Virology 2010; 407 (2):237-46 Mutations of an antibody binding energy hot spot on domain III of the dengue 2 envelope glycoprotein exploited for neutralization escape.
Matsui K et al. Virology 2009; 384 (1):16-20 Characterization of dengue complex-reactive epitopes on dengue 3 virus envelope protein domain III.
Application : ELISA
Gromowski GD et al. J Virol 2008; 82 (17):8828-37 Characterization of dengue virus complex-specific neutralizing epitopes on envelope protein domain III of dengue 2 virus.
Application : ELISA
Volk DE et al. Virology 2007; 364 (1):147-54 Solution structure of the envelope protein domain III of dengue-4 virus.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399