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Dishevelled 2 antibody [N1N3]

Comparison
Anti-Dishevelled 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111156
Anti-Dishevelled 2 antibody [N1N3] used in Western Blot (WB). GTX111156
Anti-Dishevelled 2 antibody [N1N3] used in Western Blot (WB). GTX111156
Anti-Dishevelled 2 antibody [N1N3] used in Western Blot (WB). GTX111156
Anti-Dishevelled 2 antibody [N1N3] used in Western Blot (WB). GTX111156

Cat. No. GTX111156

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Dishevelled 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2 , 293T , A431 , HeLa , HepG2 , Jurkat , Raji , NCI-H929

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Dishevelled 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618250

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dishevelled segment polarity protein 2

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Dishevelled 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111156

GTX111156 ICC/IF Image

Dvl-2 antibody [N1N3] detects Dvl-2 protein at cytoplasm by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dvl-2 protein stained by Dvl-2 antibody [N1N3] (GTX111156) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Dishevelled 2 antibody [N1N3] used in Western Blot (WB). GTX111156

GTX111156 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dishevelled 2 antibody [N1N3] (GTX111156) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dishevelled 2 antibody [N1N3] used in Western Blot (WB). GTX111156

GTX111156 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dishevelled 2 antibody [N1N3] (GTX111156) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dishevelled 2 antibody [N1N3] used in Western Blot (WB). GTX111156

GTX111156 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dishevelled 2 antibody [N1N3] (GTX111156) diluted at 1:5000 and competitor's antibody (SC-13974) diluted by 1:200.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Dishevelled 2 antibody [N1N3] used in Western Blot (WB). GTX111156

GTX111156 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dishevelled 2 antibody [N1N3] (GTX111156) diluted at 1:10000 and competitor's antibody (sc-13974) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

Application Reference

Anja Bufe et al. Proc Natl Acad Sci U S A 2021; 118 (34) Wnt signaling recruits KIF2A to the spindle to ensure chromosome congression and alignment during mitosis.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169