E-Cadherin antibody [GT477]

Name: E-Cadherin antibody [GT477]
Brand: GeneTex
SKU: GTX629691
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-E-Cadherin antibody [GT477] used in IHC (Paraffin sections) (IHC-P). GTX629691

Anti-E-Cadherin antibody [GT477] used in Western Blot (WB). GTX629691

Anti-E-Cadherin antibody [GT477] used in Flow cytometry (FACS). GTX629691

Anti-E-Cadherin antibody [GT477] used in Immunoprecipitation (IP). GTX629691

Anti-E-Cadherin antibody [GT477] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629691

Cat. No. GTX629691

Reference 4

Host	Mouse
Clonality	Monoclonal
Clone Name	GT477
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, FACS, IP
Reactivity	Human, Mouse, Rat, Dog
Package	100 μl ($399), 25 μl ($169)

See all E-Cadherin products

View product citations for antibody GTX629691 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
FACS	1:50-1:200
IP	1:100-1:500

Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888129

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO

Cellular Localization

Cell junction , Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]

Database

Gene ID: 999 CDH1
UniProt: P12830 CDH1

Research Area

DATA IMAGES

GTX629691 IHC-P Image

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
E-Cadherin antibody [GT477] (GTX629691) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX629691 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT477] (GTX629691) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629691 FACS Image

E-Cadherin antibody [GT477] (GTX629691) detects E-Cadherin protein by flow cytometry analysis.
Sample: MCF-7 cell.
Black: Unlabelled sample was used as a control.
Red: E-Cadherin antibody [GT477] (GTX629691) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

GTX629691 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT477] (GTX629691) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629691 IP Image

E-adherin antibody [GT477] immunoprecipitates E-adherin protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. Control with 3 μg of preimmune Mouse IgG
B. Immunoprecipitation of E-adherin protein by 3 μg E-adherin antibody [GT477] (GTX629691)
5 % SDS-PAGE
The immunoprecipitated E-adherin protein was detected by E-adherin antibody [GT477] (GTX629691) diluted at 1:500.
[EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]

GTX629691 IHC-P Image

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane and cytoplasm in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
E-Cadherin antibody [GT477] (GTX629691) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX629691 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT477] (GTX635000) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629691 ICC/IF Image

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MCF7 cells were fixed in ice-cold MeOH for 5 min.
Green: E-Cadherin protein stained by E-Cadherin antibody [GT477] (GTX629691) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX629691 WB Image

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT477] (GTX629691) diluted at 1:2000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629691 IHC-P Image

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog skin.
E-Cadherin stained by E-Cadherin antibody [GT477] (GTX629691) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX629691 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT477] (GTX629691) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629691 ICC/IF Image

E-Cadherin antibody [GT477] detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody [GT477] (GTX629691) diluted at 1:2000.

GTX629691 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with E-Cadherin antibody [GT477] (GTX629691) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

Application Reference

A Adamiok-Ostrowska et al. Oncol Lett 2023; Agrin is a novel oncogenic protein in thyroid cancer

T?nia Lima et al. Cancers (Basel) 2022; 14 (8) Application of Proteogenomics to Urine Analysis towards the Identification of Novel Biomarkers of Prostate Cancer: An Exploratory Study.

Application : WB

Hongtao Luo et al. Front Public Health 2020; 8 : 579705 Carbon Ion Therapy Inhibits Esophageal Squamous Cell Carcinoma Metastasis by Upregulating STAT3 Through the JAK2/STAT3 Signaling Pathway.

Application : WB

Reactivity : Human

Pei YF et al. Am J Pathol 2019; (Epub) Methyl-CpG Binding Domain Protein 2 Inhibits the Malignant Characteristic of Lung Adenocarcinoma through the Epigenetic Modulation of 10 to 11 Translocation 1 and miR-200s.

Application : WB

Reactivity : Human

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