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ERK2 antibody [N2C3]

KO/KD IP/MS
Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094
Anti-ERK2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113094
Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094
Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094
Anti-ERK2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113094
Anti-ERK2 antibody [N2C3] used in Immunoprecipitation (IP). GTX113094
Anti-ERK2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113094
Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094
Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094
Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094
Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094

Cat. No. GTX113094

Reference 15
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat, Cat, Dog, Pig
Package
100 μl ($399),
25 μl ($169)
See all ERK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

H1299 , HeLa , Mouse brain

Product Note

IP/MS validation was supported by references (PMID:30377410)

Predict Reactivity

Zebrafish, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036872

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 1 , ERK , ERK-2 , ERK2 , ERT1 , MAPK2 , P42MAPK , PRKM1 , PRKM2 , p38 , p40 , p41 , p41mapk , p42-MAPK

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094

GTX113094 WB Image

KO/KD

Wild-type (WT) and ERK2 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK2 antibody [N2C3] (GTX113094) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERK2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113094

GTX113094 IHC-P Image

ERK2 antibody [N2C3] detects ERK2 protein at cytosol and nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
ERK2 antibody [N2C3] (GTX113094) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094

GTX113094 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERK2 antibody (GTX113094) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094

GTX113094 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERK2 antibody (GTX113094) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERK2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113094

GTX113094 IHC-P Image

ERK2 antibody detects ERK2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat mammary gland.
ERK2 stained by ERK2 antibody (GTX113094) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ERK2 antibody [N2C3] used in Immunoprecipitation (IP). GTX113094

GTX113094 IP Image

ERK2 antibody [N2C3] immunoprecipitates MAPK1 protein in IP experiments.
IP samples: HeLa whole cell extract
A. 30 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MAPK1 protein by 4 μg ERK2 antibody [N2C3] (GTX113094)
10 % SDS-PAGE
The immunoprecipitated MAPK1 protein was detected by ERK2 antibody [N2C3] (GTX113094) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-ERK2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113094

GTX113094 IHC-P Image

ERK2 antibody [N2C3] detects ERK2 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ERK2 antibody [N2C3] (GTX113094) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094

GTX113094 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
10% SDS PAGE
GTX113094 diluted at 1:1000

Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094

GTX113094 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX113094 diluted at 1:5000

Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094

GTX113094 WB Image

The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799

Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094

GTX113094 WB Image

The data was published in the journal Cancers (Basel) in 2019. PMID: 31835799

REFERENCE

Application Reference

Lee H.P. et al. ournal of Functional Foods 2021; 86 : 104712 Cordycerebroside A suppresses VCAM-dependent monocyte adhesion in osteoarthritis synovial fibroblasts by inhibiting MEK/ERK/AP-1 signaling.
Application : WB
Reactivity : Human
Liu JF et al. J Cell Physiol 2020; (Epub) PM2.5 facilitates IL-6 production in human osteoarthritis synovial fibroblasts via ASK1 activation.
Application : WB
Reactivity : Human
Nguyen HD et al. Cancers (Basel) 2019; 11 (12) The α9 Nicotinic Acetylcholine Receptor Mediates Nicotine-Induced PD-L1 Expression and Regulates Melanoma Cell Proliferation and Migration.
Mitra J et al. Mol Brain 2019; 12 (1) : 55 Loss of endosomal recycling factor RAB11 coupled with complex regulation of MAPK/ERK/AKT signaling in postmortem spinal cord specimens of sporadic amyotrophic lateral sclerosis patients.
Huang SY et al. Mol Ther Nucleic Acids 2019; 17 : 10-23 Novel Role for miR-1290 in Host Species Specificity of Influenza A Virus.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

ERK2 antibody [N2C3] Cat. No. GTX113094

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-ERK2 antibody [N2C3] used in Western Blot (WB). GTX113094
Date : Anonymous submitted on 22-Oct-2012
Score :
Application Tested : WB
Sample Species : Rat
Sample : SD Rat brain thalamus tissue,amygdala tissue
Amount used : 20μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Submit a Review
Application Reference
Lee H.P. et al. ournal of Functional Foods 2021; 86:104712 Cordycerebroside A suppresses VCAM-dependent monocyte adhesion in osteoarthritis synovial fibroblasts by inhibiting MEK/ERK/AP-1 signaling.
Application : WB
Reactivity : Human
Liu JF et al. J Cell Physiol 2020; (Epub) PM2.5 facilitates IL-6 production in human osteoarthritis synovial fibroblasts via ASK1 activation.
Application : WB
Reactivity : Human
Nguyen HD et al. Cancers (Basel) 2019; 11 (12) The α9 Nicotinic Acetylcholine Receptor Mediates Nicotine-Induced PD-L1 Expression and Regulates Melanoma Cell Proliferation and Migration.
Mitra J et al. Mol Brain 2019; 12 (1):55 Loss of endosomal recycling factor RAB11 coupled with complex regulation of MAPK/ERK/AKT signaling in postmortem spinal cord specimens of sporadic amyotrophic lateral sclerosis patients.
Huang SY et al. Mol Ther Nucleic Acids 2019; 17:10-23 Novel Role for miR-1290 in Host Species Specificity of Influenza A Virus.
Application : WB
Reactivity : Human
Sikorski K et al. Nat Methods 2018; 15 (11):909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Chen CF et al. Oncology Letters 2018;:6323-6330 synergistic inhibitory effects of * and curcumin on human *-resistant oral cancer car cells through intrinsic apoptotic process.
Application : WB
Reactivity : Human
Nana AW et al. Horm Cancer 2018; (Epub) Nano-Diamino-Tetrac (NDAT) Enhances Resveratrol-Induced Antiproliferation by Action on the RRM2 Pathway in Colorectal Cancers.
Application : WB
Reactivity : Human
Jui-Cheng Tsai et al. Journal of Functional Foods 2018; 48:283-296 Extracts of Cerbera manghas L. effectively inhibit the viability of glioblastoma cell lines and their cancer stemloids in vitro and in mouse xenograft model.
Application : WB
Reactivity : Human
Cai H et al. J Nat Med 2017; (Epub) 18β-glycyrrhetinic acid inhibits migration and invasion of human gastric cancer cells via the ROS/PKC-α/ERK pathway.
Application : WB
Reactivity : Human
Chin YT et al. Mediators Inflamm 2016; 2016:6953459 2,3,5,4'-Tetrahydroxystilbene-2-O-β-glucoside Isolated from Polygoni Multiflori Ameliorates the Development of Periodontitis.
Application : WB
Reactivity : Human
Lin HY et al. Steroids 2016; (Epub) Actions of l-thyroxine and Nano-diamino-tetrac (Nanotetrac) on PD-L1 in cancer cells.
Application : WB
Reactivity : Human
Sinanoglu O et al. Urology 2012; 80 (6):1392.e1-6 The protective effects of spirulina in * induced nephrotoxicity and urotoxicity in rats.
Application : WB
Reactivity : Mouse
Tseng HL et al. Toxicol Appl Pharmacol 2012; 264 (1):104-13 Quercetin 3-O-methyl ether protects FL83B cells from copper induced oxidative stress through the PI3K/Akt and MAPK/Erk pathway.
Application : WB
Reactivity : Mouse
Yun-Hong Y et al. Mol Cell Proteomics 2011; 10 (10):M110.007138 A study of the spatial protein organization of the postsynaptic density isolated from porcine cerebral cortex and cerebellum.
Application : WB
Reactivity : Pig
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169