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EasyBlot anti sheep IgG (HRP)

EasyBlot anti sheep IgG (HRP). GTX628906-01

Cat. No. GTX628906-01

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Isotype

 IgG2a

Application

 WB

Reactivity

 Sheep
Package
250 μl ($199),
50 μl ($89)
See all Sheep IgG products
PRODUCT

Summary

EasyBlot anti-Sheep IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of Sheep IgG and does not bind the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). EasyBlot is simple to use and results in Western Blot detection of only the target band in your IP experiments. Exercise caution when using Protein A/G for IP with EasyBlot. Protein A/G contaminants bind to sheep IgG with high affinity and may cause contaminating bands with a molecular weight similar to IgG heavy chain or light chian.

To minimize background from Protein A/G contamination, we strongly recommend the use of EasyBlot Kit anti-sheep IgG (Optimized for Prot A/G) (GTX228911-01) or EasyBloker (GTX425858) to minimize protein A/G contamination.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.05M Tris, 0.15M NaCl, 1%BSA, 40% Glycerol

Preservative

0.005% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Sheep

Target Isotype

IgG

Purification

Affinity purified by Protein G.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

EasyBlot anti sheep IgG (HRP). GTX628906-01

GTX628906-01 WB Image

EasyBlot anti-Sheep IgG (HRP) detects native sheep IgG protein by Western blot analysis.
A. 0.2 μg native sheep IgG
B. 0.1 μg native sheep IgG
C. 1 μg denatured sheep IgG
D. 2 μg denatured sheep IgG
E. 4 μg denatured sheep IgG
F. 8 μg denatured sheep IgG
10 % SDS-PAGE
EasyBlot anti-Sheep IgG (HRP) (GTX628906-01) dilution: 1:2000

REFERENCE

Application Reference

Hung YH et al. Am J Cancer Res 2022; 12 (2) : 713-728 Semaphorin 4C promotes motility and immunosuppressive activity of cancer cells via CRMP3 and PD-L1.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 199
$ 89