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HA tag antibody

Anti-HA tag antibody used in Immunoprecipitation (IP). GTX115044
Anti-HA tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115044
Anti-HA tag antibody used in Western Blot (WB). GTX115044
Anti-HA tag antibody used in ELISA (ELISA). GTX115044
Anti-HA tag antibody used in Western Blot (WB). GTX115044
Anti-HA tag antibody used in Western Blot (WB). GTX115044
Anti-HA tag antibody used in Western Blot (WB). GTX115044

Cat. No. GTX115044

Reference 55
5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ELISA, ChIP assay

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all HA tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IP 1:100-1:500
ELISA 1:1000-1:10000
ChIP assay Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to HA tag

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10622369

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HA tag antibody , HA antibody , HA epitope tag antibody

Research Area

DATA IMAGES

Anti-HA tag antibody used in Immunoprecipitation (IP). GTX115044

GTX115044 IP Image

Immunoprecipitation of HA tag protein from 293T whole cell extracts using 5 μg of HA tag antibody (GTX115044).
Western blot analysis was performed using HA tag antibody (GTX115044) diluted at 1:2500.
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

Anti-HA tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115044

GTX115044 ICC/IF Image

Immunofluorescence analysis of HA-recombinant protein transfected HeLa cell which fixed with paraformaldehyde, using HA tag(GTX115044) antibody at 1:500 dilution.

Anti-HA tag antibody used in Western Blot (WB). GTX115044

GTX115044 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HA tag antibody (GTX115044) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HA tag antibody used in ELISA (ELISA). GTX115044

GTX115044 ELISA Image

An ELISA plate is coated with 50 μL of a HA-Tag conjugated BSA at concentration of 5 μg/mL. The peptide conjugated carrier protein is coated and detected with anti-HA tag antibody (GTX115044) at concentration ranged from 3.1 to 200 ng/mL.

Anti-HA tag antibody used in Western Blot (WB). GTX115044

GTX115044 WB Image

The data was published in the journal Front Microbiol in 2019. PMID: 31969874

Anti-HA tag antibody used in Western Blot (WB). GTX115044

GTX115044 WB Image

The data was published in the journal Nat Commun in 2016.PMID: 26783207

Anti-HA tag antibody used in Western Blot (WB). GTX115044

GTX115044 WB Image

The data was published in the journal Nat Commun in 2016.PMID: 26783207

REFERENCE

Application Reference

H Heo et al. Autophagy 2023; : 1-16 TRIM22 facilitates autophagosome-lysosome fusion by mediating the association of GABARAPs and PLEKHM1
Takemoto R et al. J Virol 2023; 97 (5) : e0034023 Interaction of the Hemagglutinin Stalk Region with Cell Adhesion Molecule (CADM) 1 and CADM2 Mediates the Spread between Neurons and Neuropathogenicity of Measles Virus with a Hyperfusogenic Fusion Protein.
Dacheux D et al. Elife 2023; 12 : RP87698 Novel axonemal protein ZMYND12 interacts with TTC29 and DNAH1, and is required for male fertility and flagellum function
Application : ICC/IF
Huang TH et al. Front Cell Neurosci 2023; 17 : 1146278 Differential expression of GABA(A) receptor subunits δ and α6 mediates tonic inhibition in parvalbumin and somatostatin interneurons in the mouse hippocampus.
Y Liu et al. Cell Rep 2023; 42 (8) : 112852 Small cytosolic double-stranded DNA represses cyclic GMP-AMP synthase activation and induces autophagy
Application : WB
Show More +
Submit a Reference

REVIEW

HA tag antibody Cat. No. GTX115044

Rating
( Average 4.8 based on 5 users reviews)
Application
Western Blot(WB)
( Average 4.8 based on 4 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-HA tag antibody used in Western Blot (WB). GTX115044
Date : Anonymous submitted on 18-Dec-2023
Score :
Application Tested : WB
Sample : 293T cells with/without HA-tagged target protein transfection
Amount used : 100μg
Blocking : 2%BSA, 25°C, 1Hr
Primary Antibody : 1:6000, 25°C, > 16Hr
Anti-HA tag antibody used in Western Blot (WB). GTX115044
Date : Chen-I Hsu submitted on 16-Sep-2021
Score :
Application Tested : WB
Sample : A549 cells
Amount used : 50μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 25°C, 1Hr
Secondary Antibody : GTX213110-01, 1:2000, 25°C, 1Hr
Lane Description : The A549 cells were transfected with HA-tagged DNAJB6 cDNA and harvested at 24h post-transfection. The DNAJB6 antibody recognized both endogenous and HA-tagged DNAJB6 while the Genetex HA-tag antibody only recognized one band.
Anti-HA tag antibody used in Western Blot (WB). GTX115044
Date : Anonymous submitted on 06-May-2021
Score :
Application Tested : WB
Sample : A549 cells
Amount used : 10 μg
Blocking : 5% skim milk in TBST, 27°C, 1Hr
Primary Antibody : 1:5000, 4°C, 12Hr
Secondary Antibody : GTX213110-01, 1:5000, 27°C, 2Hr
Lane Description : Two groups (HA protein -/+) of whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HA antibody (GTX115044) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-HA tag antibody used in Western Blot (WB). GTX115044
Date : Anonymous submitted on 27-Oct-2016
Score :
Application Tested : WB
Sample : HA-human ApoA1-transfected HeLa cells
Amount used : 15μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:20000, 4°C, 16Hr
Anti-HA tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115044
Date : Anonymous submitted on 27-Oct-2016
Score :
Application Tested : ICC/IF
Sample : HA-human ApoA1-transfected HeLa cells
Blocking : 3% BSA, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.05% saponin
Primary Antibody : 1:2000, 4°C, 16Hr
Submit a Review
Application Reference
H Heo et al. Autophagy 2023;:1-16 TRIM22 facilitates autophagosome-lysosome fusion by mediating the association of GABARAPs and PLEKHM1
Takemoto R et al. J Virol 2023; 97 (5):e0034023 Interaction of the Hemagglutinin Stalk Region with Cell Adhesion Molecule (CADM) 1 and CADM2 Mediates the Spread between Neurons and Neuropathogenicity of Measles Virus with a Hyperfusogenic Fusion Protein.
Dacheux D et al. Elife 2023; 12:RP87698 Novel axonemal protein ZMYND12 interacts with TTC29 and DNAH1, and is required for male fertility and flagellum function
Application : ICC/IF
Huang TH et al. Front Cell Neurosci 2023; 17:1146278 Differential expression of GABA(A) receptor subunits δ and α6 mediates tonic inhibition in parvalbumin and somatostatin interneurons in the mouse hippocampus.
Y Liu et al. Cell Rep 2023; 42 (8):112852 Small cytosolic double-stranded DNA represses cyclic GMP-AMP synthase activation and induces autophagy
Application : WB
Zhao B et al. Cell Oncol (Dordr) 2023; 46 (3):717-733 USP13 promotes breast cancer metastasis through FBXL14-induced Twist1 ubiquitination.
M Huang et al. Cell Rep 2023; 42 (6):112667 LRP12 is an endogenous transmembrane inactivator of α4 integrins
Y Li et al. Cell Death Dis 2023; KLF12 promotes the proliferation of breast cancer cells by reducing the transcription of p21 in a p53-dependent and p53-independent manner
Application : WB, IP
Xia K et al. Cell Death Dis 2023; 14 (4):250 Cryptochrome 2 acetylation attenuates its antiproliferative effect in breast cancer.
Application : WB
Lin CY et al. Sci Adv 2023; 9 (14):eade9944 Small-molecule PIK-93 modulates the tumor microenvironment to improve immune checkpoint blockade response.
Application : WB, IP
Alvim JC et al. Nat Commun 2023; 14 (1):1612 The K/HDEL receptor does not recycle but instead acts as a Golgi-gatekeeper.
Application : IP
Liao WT et al. J Biol Chem 2023; 299 (12):105455 CBAP regulates the function of Akt-associated TSC protein complexes to modulate mTORC1 signaling.
Chang CK et al. J Fungi (Basel) 2022; 8 (11) The Role of Sfp1 in Candida albicans Cell Wall Maintenance.
Application : ChIP assay
Takemoto R et al. J Virol 2022; 96 (3):e0194921 Short-Stalk Isoforms of CADM1 and CADM2 Trigger Neuropathogenic Measles Virus-Mediated Membrane Fusion by Interacting with the Viral Hemagglutinin.
Domenico Azarnia Tehran et al. Sci Adv 2022; 8 (21):eabl5032 Selective endocytosis of Ca2+-permeable AMPARs by the Alzheimer's disease risk factor CALM bidirectionally controls synaptic plasticity.
Anshu Agarwal et al. Microbiol Spectr 2022; 10 (3):e0083022 Japanese Encephalitis Virus NS4A Protein Interacts with PTEN-Induced Kinase 1 (PINK1) and Promotes Mitophagy in Infected Cells.
Application : ICC/IF
Christine E Broster Reix et al. Microbiol Spectr 2021; 9 (2):e0091521 Intrabody-Induced Cell Death by Targeting the T. brucei Cytoskeletal Protein TbBILBO1.
Application : ICC/IF
Aman S et al. Cell Death Discov 2021; 7 (1):351 DACH1 inhibits breast cancer cell invasion and metastasis by down-regulating the transcription of matrix metalloproteinase 9.
Application : WB
Chen WT et al. J Biomed Sci 2021; 28 (1):81 Elp1 facilitates RAD51-mediated homologous recombination repair via translational regulation.
Application : WB
Yang Y et al. Cell Death Discov 2021; 7 (1):328 FBXL10 promotes EMT and metastasis of breast cancer cells via regulating the acetylation and transcriptional activity of SNAI1.
Li X et al. NPJ Precis Oncol 2021; 5 (1):20 Acetylation of ELF5 suppresses breast cancer progression by promoting its degradation and targeting CCND1.
Application : WB, IP
Lee SA et al. Viruses 2021; 13 (3) Non-Metabolic Functions of PKM2 Contribute to Cervical Cancer Cell Proliferation Induced by the HPV16 E7 Oncoprotein.
Application : WB
Tsai MH et al. Acta Neuropathol Commun 2020; 8 (1):106 Impairment in dynein-mediated nuclear translocation by BICD2 C-terminal truncation leads to neuronal migration defect and human brain malformation.
Seo J et al. Commun Biol 2020; 3 (1):638 Fatty-acid-induced FABP5/HIF-1 reprograms lipid metabolism and enhances the proliferation of liver cancer cells.
Yao Z et al. Nat Commun 2020; 11 (1):2440 Split Intein-Mediated Protein Ligation for detecting protein-protein interactions and their inhibition.
Su YC et al. FASEB J 2020; 34 (6):7283-7294 MicroRNA-155 inhibits dengue virus replication by inducing heme oxygenase-1-mediated antiviral interferon responses.
Application : WB
Constantino-Jonapa LA et al. Parasitol Res 2020; 119 (5):1629-1640 EhRab21 associates with the Golgi apparatus in Entamoeba histolytica.
Application : WB
Ye J et al. Cell Signal 2020; 69:109567 Nuclear import of NLS- RARα is mediated by importin α/β.
Application : WB
Cao C et al. Front Microbiol 2019; 10:3040 BICP0 Negatively Regulates TRAF6-Mediated NF-庥B and Interferon Activation by Promoting K48-Linked Polyubiquitination of TRAF6.
Application : WB
Chang CH et al. Dev Cell 2019; 48 (2):184-199.e5 Atoh1 Controls Primary Cilia Formation to Allow for SHH-Triggered Granule Neuron Progenitor Proliferation.
Chen WC et al. J Viral Hepat 2019; (Epub) MicroRNA-let-7c suppresses hepatitis C virus replication by targeting Bach1 for induction of haem oxygenase-1 expression.
Application : WB
Qin J et al. Avian Pathol 2018;:1月10日 Biological characteristics and immunological properties in Muscovy ducks of H5N6 virus-like particles composed of HA-TM/HA-TMH3 and M1.
Application : WB
Lin CK et al. J Infect Dis 2018; (Epub) Prostasin impairs epithelial growth factor receptor activation to suppress dengue virus propagation.
Application : WB
Yang D et al. Nat Commun 2018; 9 (1):3052 Sensing of cytosolic LPS through caspy2 pyrin domain mediates noncanonical inflammasome activation in zebrafish.
Application : WB
Lee HY et al. Oncotarget 2018; 9 (17):13193-13205 EIF3C-enhanced exosome secretion promotes angiogenesis and tumorigenesis of human hepatocellular carcinoma.
Application : WB
Liu GT et al. FASEB J 2018;:fj201701253R Improving nuclear envelope dynamics by EBV BFRF1 facilitates intranuclear component clearance through autophagy.
Application : ICC/IF
Omoto T et al. Oncogene 2017; (Epub) The impact of stromal Hic-5 on the tumorigenesis of colorectal cancer through lysyl oxidase induction and stromal remodeling.
Application : WB
Wang CY et al. Am J Cancer Res 2017; 7 (11):2103-2120 PSMB5 plays a dual role in cancer development and immunosuppression.
Application : WB
Kubo Y et al. Front Microbiol 2017; 8:1653 Rab3a-Bound CD63 Is Degraded and Rab3a-Free CD63 Is Incorporated into HIV-1 Particles.
Application : WB
Barajas D et al. PLoS One 2017; 12 (3):e0173010 Generation of infectious recombinant Adeno-associated virus in Saccharomyces cerevisiae.
Su KH et al. Nat Cell Biol 2016; 18 (5):527-39 HSF1 critically attunes proteotoxic stress sensing by mTORC1 to combat stress and promote growth.
Application : WB
Tien SC et al. Nat Commun 2016; 7:10420 The Shp2-induced epithelial disorganization defect is reversed by HDAC6 inhibition independent of Cdc42.
Application : WB
Braso-Maristany F et al. Nat Med 2016; 22 (11):1303-1313 PIM1 kinase regulates cell death, tumor growth and chemotherapy response in triple-negative breast cancer.
Cao XL et al. Protein Expr Purif 2016; 130:81-89 Expression and purification of mouse Ttyh1 fragments as antigens to generate Ttyh1-specific monoclonal antibodies.
Lee CP et al. J Virol 2016; The Ubiquitin Ligase Itch and Ubiquitination Regulate BFRF1-mediated Nuclear Envelope Modification for EBV Maturation.
Application : ICC/IF
Lin TJ et al. Sci Rep 2016; 6:27768 Inhibition of endosomal fusion activity of influenza virus by Rheum tanguticum (da-huang).
Application : WB
Skieterska K et al. Cell Signal 2016; 28 (8):1001-1014 Characterization of the interaction between the * D4 receptor, KLHL12 and β-arrestins.
Application : WB
Chang AT et al. Genes Dev 2015; 29 (6):603-16 An evolutionarily conserved DNA architecture determines target specificity of the TWIST family bHLH transcription factors.
Chen YA et al. Sci Rep 2015; 5 (Epub):10667 The Cullin 4A/B-DDB1-Cereblon E3 Ubiquitin Ligase Complex Mediates the Degradation of CLC-1 Chloride Channels.
Application : WB
Hyvarinen S et al. J Biol Chem 2014; 289 (7):4295-306 Recognition of Malondialdehyde-modified Proteins by the C Terminus of Complement Factor H Is Mediated via the Polyanion Binding Site and Impaired by Mutations Found in Atypical Hemolytic Uremic Syndrome.
Ho LJ et al. PLoS One 2013; 8 (12):e82033 Ginkgo biloba extract individually inhibits JNK activation and induces c-Jun degradation in human chondrocytes: potential therapeutics for osteoarthritis.
Application : WB
Tsao HW et al. Proc Natl Acad Sci U S A 2013; 110 (39):15776-81 Ets-1 facilitates nuclear entry of NFAT proteins and their recruitment to the IL-2 promoter.
Application : WB
Lee CP et al. PLoS Pathog 2012; 8 (9):e1002904 The ESCRT machinery is recruited by the viral BFRF1 protein to the nucleus-associated membrane for the maturation of Epstein-Barr Virus.
Application : ICC/IF
Lin CS et al. Cardiovasc Res 2012; 95 (3):346-55 PKCδ signalling regulates SR-A and CD36 expression and foam cell formation.
Wang X et al. Cancer Sci 2012; 103 (3):515-21 calcineurin subunit b upregulates β-* production by phosphorylation of * regulatory factor 3 via toll-like receptor 4.
Application : WB
SDS
PBS.pdf
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Proclin.pdf
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