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Histone H3K27me3 (Tri-methyl Lys27) antibody

Orthogonal
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX121184
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Frozen sections) (IHC-Fr). GTX121184
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Dot blot (Dot). GTX121184
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Western Blot (WB). GTX121184
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX121184
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX121184
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121184
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Western Blot (WB). GTX121184
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Whole mount) (IHC-Wm). GTX121184
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX121184
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121184
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121184
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX121184
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Western Blot (WB). GTX121184
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Western Blot (WB). GTX121184
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Western Blot (WB). GTX121184

Cat. No. GTX121184

Reference 11
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, Dot, EM

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC-Wm 1:100-1:500
Dot Assay dependent
EM Assay dependent
Not tested in other applications.

Positive Control

Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding tri-methyl Lys27 of human Histone H3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618572

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX121184

GTX121184 IHC-P Image

Histone H3K27me3 (trimethyl Lys27) antibody detects Histone H3K27me3 (trimethyl Lys27) protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Frozen sections) (IHC-Fr). GTX121184

GTX121184 IHC-Fr Image

Immunofluorescence photomicrographs of frozen sections of mouse liver.
Red: Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight594) (GTX213110-05).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Dot blot (Dot). GTX121184

GTX121184 Dot Image

Various peptide samples were spotted onto positively charged nylon membrane, and blotted with Histone H3 (tri-Methyl Lys27) antibody (GTX121184) diluted at 1:2500.

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Western Blot (WB). GTX121184

GTX121184 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) diluted at 1:1000.

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX121184

GTX121184 IHC-P Image

Histone H3K27me3 (Tri-methyl Lys27) antibody detects Histone H3K27me3 (Tri-methyl Lys27) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody (GTX121184) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX121184

GTX121184 IHC-P Image

Histone H3K27me3 (trimethyl Lys27) antibody detects Histone H3K27me3 (trimethyl Lys27) protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121184

GTX121184 ICC/IF Image

Histone H3K27me3 (Tri-methyl Lys27) antibody detects Histone H3K27me3 (Tri-methyl Lys27) protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody (GTX121184) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Western Blot (WB). GTX121184

GTX121184 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27me3 (Tri-methyl Lys27) antibody (GTX121184) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Whole mount) (IHC-Wm). GTX121184

GTX121184 IHC-Wm Image

Histone H3K27me3 (trimethyl Lys27) antibody detects Histone H3K27me3 (trimethyl Lys27) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) dilution: 1:100.

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX121184

GTX121184 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Histon H3 (tri-Methyl K27)(GTX121184) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121184

GTX121184 ICC/IF Image

Histone H3K27me3 (Tri-methyl Lys27) antibody detects Histone H3K27me3 (Tri-methyl Lys27) protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody (GTX121184) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121184

GTX121184 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using Histone H3 (tri-Methyl K27)(GTX121184) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX121184

GTX121184 IHC-P Image

Histone H3K27me3 (Tri-methyl Lys27) antibody detects Histone H3K27me3 (Tri-methyl Lys27) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody (GTX121184) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Western Blot (WB). GTX121184

GTX121184 WB Image

Orthogonal

U87 cells were grown under normoxic (DMEM 10% FBS, 16% O2), hypoxic (DMEM 10% FBS, 1% O2), and neurosphere conditions (DMEM/F12, B-27 supplement, growth factor (10ng/ml FGF and 20ng/ml EGF)). Cell lysate were Western blotted for H3K27me3 and Histone-H3 (loading control). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Western Blot (WB). GTX121184

GTX121184 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27me3 (Tri-methyl Lys27) antibody (GTX121184) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Western Blot (WB). GTX121184

GTX121184 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27me3 (Tri-methyl Lys27) antibody (GTX121184) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

C Casali et al. Eur J Cell Biol 2023; 103 (1) : 151373 Impact of heat and cold shock on epigenetics and chromatin structure
Application : EM
Perrigue PM et al. Cancers (Basel) 2020; 12 (7) Cancer Stem Cell-Inducing Media Activates Senescence Reprogramming in Fibroblasts.
Zhang T et al. Int J Nanomedicine 2020; 15 : 1457-1468 Anticancer Effects of Zinc Oxide Nanoparticles Through Altering the Methylation Status of Histone on Bladder Cancer Cells.
Application : WB
Reactivity : Human
Hasebe T et al. Gen Comp Endocrinol 2020; 292 : 113441 Stem cell development involves divergent thyroid hormone receptor subtype expression and epigenetic modifications in the Xenopus metamorphosing intestine.
Application : IHC-P
Reactivity : Human
Tanr?kulu B et al. Childs Nerv Syst 2020; (Epub) Is H3K27me3 status really a strong prognostic indicator for pediatric posterior fossa ependymomas? A single surgeon, single center experience.
Application : IHC-P
Reactivity : Human
Show More +
Submit a Reference

REVIEW

Histone H3K27me3 (Tri-methyl Lys27) antibody Cat. No. GTX121184

Rating
( Average 4.3 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4 based on 2 users reviews)
Electron microscopy(EM)
( Average 5 based on 1 users reviews)
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Western Blot (WB). GTX121184
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:100, 4°C, 16Hr
Lane Description : Lane 1: MG63 whole cell lysate, Lane 2: doxorubicin resistant MG63 cell line, Lane 3: Cisplatin resistant MG63 cell line
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Electron microscopy (EM). GTX121184
Date : Anonymous submitted on 25-Jul-2016
Score :
Application Tested : EM
Sample Species : Rat
Sample : liver tissue
Blocking : Normal Goat Serum in PBS, 25°C, 0.08Hr
Fixation : 4% PFA in S?rensen phosphate buffer (pH 7.2)
Permeabilization /Antigen retrieval : 0.1% BSA in PBST
Primary Antibody : 1:50, 4°C, 16Hr
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Western Blot (WB). GTX121184
Date : Anonymous submitted on 20-Jan-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 60μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
C Casali et al. Eur J Cell Biol 2023; 103 (1):151373 Impact of heat and cold shock on epigenetics and chromatin structure
Application : EM
Perrigue PM et al. Cancers (Basel) 2020; 12 (7) Cancer Stem Cell-Inducing Media Activates Senescence Reprogramming in Fibroblasts.
Zhang T et al. Int J Nanomedicine 2020; 15:1457-1468 Anticancer Effects of Zinc Oxide Nanoparticles Through Altering the Methylation Status of Histone on Bladder Cancer Cells.
Application : WB
Reactivity : Human
Hasebe T et al. Gen Comp Endocrinol 2020; 292:113441 Stem cell development involves divergent thyroid hormone receptor subtype expression and epigenetic modifications in the Xenopus metamorphosing intestine.
Application : IHC-P
Reactivity : Human
Tanr?kulu B et al. Childs Nerv Syst 2020; (Epub) Is H3K27me3 status really a strong prognostic indicator for pediatric posterior fossa ependymomas? A single surgeon, single center experience.
Application : IHC-P
Reactivity : Human
Blin G et al. Nutrients 2020; 12 (1) Maternal Exposure to High-Fat Diet Induces Long-Term Derepressive Chromatin Marks in the Heart.
Application : WB
Reactivity : Rat
Tsuyama N et al. Hematol Oncol 2017; Epstein-Barr virus-negative extranodal "true" natural killer-cell lymphoma harbouring a KDM6A mutation.
Application : IHC-P
Reactivity : Human
Mirzamohammadi F et al. Nat Commun 2016; 7:12047 Polycomb repressive complex 2 regulates skeletal growth by suppressing Wnt and TGF-β signalling.
Application : WB
Reactivity : Mouse
Perrigue PM et al. Mol Cancer Res 2015; 13 (4):636-50 The histone demethylase jumonji coordinates cellular senescence including secretion of neural stem cell-attracting cytokines.
Liu TP et al. Anticancer Drugs 2015; 26 (2):139-47 S-Adenosyl-L-methionine-competitive inhibitors of the histone methyltransferase EZH2 induce autophagy and enhance drug sensitivity in cancer cells.
Application : WB
Reactivity : Human
Jiang X et al. FASEB J 2012; 26 (8):3563-74 Maternal choline intake alters the epigenetic state of fetal cortisol-regulating genes in humans.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169