Summary
GZMB produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 235 amino acids (19-247a.a.) and having a molecular mass of 26.5kDa. (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). GZMB is expressed with an 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Form
Liquid
Buffer
PBS, 20% Glycerol, 1mM DTT
Preservative
No preservative
Storage
Store as concentrated solution. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding a carrier protein (0.1% HSA or BSA), then aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Region/Sequence
C-terminal His-Tag; GEIIGGHEAK PHSRPYMAYL MIWDQKSLKR CGGFLIRDDF VLTAAHCWGS SINVTLGAHN IKEQEPTQQF IPVKRPIPHP AYNPKNFSND IMLLQLERKA KRTRAVQPLR LPSNKAQVKP GQTCSVAGWG QTAPLGKHSH TLQEVKMTVQ EDRKCESDLR HYYDSTIELC VGDPEIKKTS FKGDSGGPLV CNKVAQGIVS YGRNNGMPPR ACTKVSSFVH WIKKTMKRYH HHHHH
Expression System
Baculovirus (Sf9 cells)
Purity
≥ 95% by SDS-PAGE.
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Synonyms
granzyme B , C11 , CCPI , CGL-1 , CGL1 , CSP-B , CSPB , CTLA1 , CTSGL1 , HLP , SECT
Database
Research Area