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United States (US)

Human IgG1 kappa isotype control

Cat. No. GTX35068

Reference 1

Host

 Human

Clonality

 Monoclonal

Isotype

 IgG1

Application

 ELISA
Package
100 μg ($109)
See all Human IgG1 kappa chain isotype control products

APPLICATION

Application Note

This item can be coated on ELISA using a dilution of 1ug/mL.

PROPERTIES

Form

Liquid

Buffer

Borate buffered saline

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Human IgG1 kappa negative control , monoclonal Human IgG1 isotype control , Human IgG1 negative control , Isotype control to Human IgG1 kappa , Hum , Isotype control for Human IgG1 kappa , Human IgG1 isotype control , Negative control for Human IgG1 kappa

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

REFERENCE

Application Reference

Rodriguez BV et al. Sci Rep 2020; 10 (1) : 18293 Staphylococcus aureus secretes immunomodulatory RNA and DNA via membrane vesicles.
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REVIEW

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Package List Price ($)
$ 109