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Human Papillomavirus type 18 E7 antibody [GT881]

Overexpression
Anti-Human Papillomavirus type 18 E7 antibody [GT881] used in Western Blot (WB). GTX634337
Anti-Human Papillomavirus type 18 E7 antibody [GT881] used in Western Blot (WB). GTX634337
Anti-Human Papillomavirus type 18 E7 antibody [GT881] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634337
Anti-Human Papillomavirus type 18 E7 antibody [GT881] used in Lateral Flow (Lateral Flow). GTX634337
Anti-Human Papillomavirus type 18 E7 antibody [GT881] used in ELISA (ELISA). GTX634337
Anti-Human Papillomavirus type 18 E7 antibody [GT881] used in ELISA (ELISA). GTX634337

Cat. No. GTX634337

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT881

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA, Lateral Flow

Reactivity

 Human papillomavirus type 18
Package
100 μl ($399),
25 μl ($169)
See all Human Papillomavirus type 18 E7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
ELISA Assay dependent
Lateral Flow Assay dependent

Note :

Lateral Flow
Capture : GTX638883, Detection : GTX634337

Sandwich ELISA
Capture : GTX638883, Detection : GTX634337 or Capture : GTX634337, Detection : GTX638883.
Not tested in other applications.

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human papillomavirus

Immunogen

Full length Human Papilloma virus type 18 E7 recombinant protein.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888440

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Human papillomavirus Protein E7 , Human papillomavirus E7 , HPV virus Protein E7 , HPV virus E7

Research Area

DATA IMAGES

Anti-Human Papillomavirus type 18 E7 antibody [GT881] used in Western Blot (WB). GTX634337

GTX634337 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 18 E7 antibody [GT881] (GTX634337) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Human Papillomavirus type 18 E7 antibody [GT881] used in Western Blot (WB). GTX634337

GTX634337 WB Image

Various whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 18 E7 antibody [GT881] (GTX634337) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Human Papillomavirus type 18 E7 antibody [GT881] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634337

GTX634337 ICC/IF Image

Human Papillomavirus type 18 E7 antibody [GT881] detects Human Papillomavirus type 18 E7 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Human Papillomavirus type 18 E7 stained by Human Papillomavirus type 18 E7 antibody [GT881] (GTX634337) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-Human Papillomavirus type 18 E7 antibody [GT881] used in Lateral Flow (Lateral Flow). GTX634337

GTX634337 Lateral Flow Image

Detection of Human Papillomavirus type 18 E7 protein by lateral flow assay using the monoclonal antibody pair.

Capture: Human Papillomavirus type 18 E7 antibody (GTX638883 [HL2522])
Detection: Human Papillomavirus type 18 E7 antibody (GTX634337 [GT881])

Samples (20 ng) :
1. Human Papilloma virus type 18 E7 protein (GTX133412-pro)
2. Lysis buffer

Anti-Human Papillomavirus type 18 E7 antibody [GT881] used in ELISA (ELISA). GTX634337

GTX634337 ELISA Image

Sandwich ELISA detection of recombinant Human Papillomavirus type 18 E7 protein, His tag (GTX133412-pro) using antibodies as below.

Capture: Human Papillomavirus type 18 E7 antibody [HL2522] (GTX638883) (5 μg/mL)
Detection: Human Papillomavirus type 18 E7 antibody [GT881] (GTX634337) (1 μg/mL)

Anti-Human Papillomavirus type 18 E7 antibody [GT881] used in ELISA (ELISA). GTX634337

GTX634337 ELISA Image

Sandwich ELISA detection of recombinant Human Papillomavirus type 18 E7 protein, His tag (GTX133412-pro) using antibodies as below.

Capture: Human Papillomavirus type 18 E7 antibody [GT881] (GTX634337) (5 μg/mL)
Detection: Human Papillomavirus type 18 E7 antibody [HL2522] (GTX638883) (1 μg/mL)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations