Integrin beta 1 / CD29 antibody

Name: Integrin beta 1 / CD29 antibody
Brand: GeneTex
SKU: GTX112971
Price: 399.00 USD

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX112971

Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX112971

Anti-Integrin beta 1 / CD29 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112971

Cat. No. GTX112971

Reference 7

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Dog
Package	100 μl ($399), 25 μl ($169)

See all Integrin beta 1 / CD29 products

View product citations for antibody GTX112971 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

human ITGB1-transfected 293T , HeLa

Predict Reactivity

Rat, Sheep, Bovine, Cat, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human Integrin beta 1 / CD29. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722978

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit beta 1 , CD29 , FNRB , GPIIA , MDF2 , MSK12 , VLA-BETA , VLAB

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Melanosome

Background

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 3688 ITGB1
UniProt: P05556 ITGB1

Research Area

DATA IMAGES

GTX112971 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Integrin beta 1 / CD29 antibody (GTX112971) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX112971 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX112971) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112971 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Integrin beta 1 / CD29(GTX112971) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX112971 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX112971) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112971 IHC-P Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX112971) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112971 WB Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human Integrin beta 1 / CD29-transfected 293T cells
7.5% SDS-PAGE
Integrin beta 1 / CD29 antibody (GTX112971) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112971 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Integrin beta 1 / CD29(GTX112971) antibody at 1:500 dilution.

GTX112971 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX112971) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.