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Lin28A antibody

Anti-Lin28A antibody used in Flow cytometry (FACS). GTX121923
Anti-Lin28A antibody used in IHC (Paraffin sections) (IHC-P). GTX121923
Anti-Lin28A antibody used in Western Blot (WB). GTX121923
Anti-Lin28A antibody used in Western Blot (WB). GTX121923

Cat. No. GTX121923

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse, Pig
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

mouse ESC , hESC

Predict Reactivity

Rat, Sheep, Bovine, Cat, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lin28A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176162

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lin-28 homolog A , CSDD1 , LIN-28 , LIN28 , ZCCHC1 , lin-28A

Cellular Localization

Cytoplasm , Nucleus , nucleolus

Background

Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.

Database

Research Area

DATA IMAGES

Anti-Lin28A antibody used in Flow cytometry (FACS). GTX121923

GTX121923 FACS Image

Flow cytometry on human embryonic stem cells, staining with Lin28A (GTX121923)antibody at 1:100 dilution(purple) or rabbit IgG (black).

Anti-Lin28A antibody used in IHC (Paraffin sections) (IHC-P). GTX121923

GTX121923 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Lin28A antibody(GTX121923) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Lin28A antibody used in Western Blot (WB). GTX121923

GTX121923 WB Image

Sample (20 μg of whole cell lysate)
A: mouse ESC
12% SDS PAGE
GTX121923 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Lin28A antibody used in Western Blot (WB). GTX121923

GTX121923 WB Image

Sample (20 μg of whole cell lysate)
A: hESC
12% SDS PAGE
GTX121923 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Lin28A antibody Cat. No. GTX121923

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 15-Sep-2014
Score :
Application Tested : WB
Sample Species : Pig
Sample : pig heart tissue
Amount used : 10μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169