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Mig-2 antibody [9E4]

KO/KD Overexpression
Anti-Mig-2 antibody [9E4] used in Western Blot (WB). GTX84507
Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507
Anti-Mig-2 antibody [9E4] used in Western Blot (WB). GTX84507
Anti-Mig-2 antibody [9E4] used in Immunoprecipitation (IP). GTX84507
Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507
Anti-Mig-2 antibody [9E4] used in Western Blot (WB). GTX84507
Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507
Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507
Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507
Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507
Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507
Anti-Mig-2 antibody [9E4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84507
Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507

Cat. No. GTX84507

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9E4

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Rat, Dog, Monkey
Package
100 μl ($519)
See all Mig-2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF 1:50
IHC-P 1:150
IP 2ug/500ul
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10727911

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fermitin family member 2 , KIND2 , MIG2 , PLEKHC1 , UNC112 , UNC112B , mig-2

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton,Nucleus

Background

Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.

Database

Research Area

DATA IMAGES

Anti-Mig-2 antibody [9E4] used in Western Blot (WB). GTX84507

GTX84507 WB Image

WB analysis of various cell lines using GTX84507 Mig-2 antibody [9E4].
Loading : 35 ug per lane

Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507

GTX84507 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84507 Mig-2 antibody [9E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Mig-2 antibody [9E4] used in Western Blot (WB). GTX84507

GTX84507 WB Image

KO/KD

WB analysis of wild type and Mig-2 knock out HeLa cell lysate(10μg per lane) using GTX84507 Mig-2 antibody [9E4].
Dilution : 1:1000

Anti-Mig-2 antibody [9E4] used in Immunoprecipitation (IP). GTX84507

GTX84507 IP Image

IP analysis of DDDDK tagged Mig-2 overexpressed HEK293T lysate using GTX84507 Mig-2 antibody [9E4].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507

GTX84507 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX84507 Mig-2 antibody [9E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Mig-2 antibody [9E4] used in Western Blot (WB). GTX84507

GTX84507 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Mig-2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84507 Mig-2 antibody [9E4].
Loading : 5 ug per lane

Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507

GTX84507 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84507 Mig-2 antibody [9E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507

GTX84507 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84507 Mig-2 antibody [9E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507

GTX84507 IHC-P Image

IHC-P analysis of human kidney carcinoma tissue using GTX84507 Mig-2 antibody [9E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507

GTX84507 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX84507 Mig-2 antibody [9E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507

GTX84507 IHC-P Image

IHC-P analysis of human colon tissue using GTX84507 Mig-2 antibody [9E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Mig-2 antibody [9E4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84507

GTX84507 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX84507 Mig-2 antibody [9E4].

Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507

GTX84507 IHC-P Image

IHC-P analysis of human ovary tissue using GTX84507 Mig-2 antibody [9E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

Application Reference

Fanny Eysert et al. bioRxiv 2019; (Epub) Alzheimer’s genetic risk factor FERMT2 (Kindlin-2) is regulated by risk allele-binding of miR-4504 and leads to synaptic dysfunction in an APP-dependent manner.
Application : WB
Sullivan SE et al. Hum Mol Genet 2018; (Epub) Candidate-based screening via gene modulation in human neurons and astrocytes implicates FERMT2 in Aβ and TAU proteostasis.
Application : WB
Reactivity : Human
Chapuis J et al. Acta Neuropathol 2016; Genome-wide, high-content siRNA screening identifies the Alzheimer's genetic risk factor FERMT2 as a major modulator of APP metabolism.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519