Mouse TGF beta 1 protein, His tag (active)

Cat. No. GTX04319-pro

Application	WB, Functional Assay, Neutralizing/Inhibition
Species	Mouse
Package	10 μg ($399)

See all TGF beta 1 protein products

Datasheet

View product citations for antibody GTX04319-pro on CiteAb

APPLICATION

Calculated MW

14.1 kDa. ( Note )

Observed MW

16 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute in 20mM Tris, 150mM NaCl pH8.0 to a concentration of 0.1-1.0 mg/mL. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl pH8.0, 0.05% sarcosyl, 5% trehalose.

Preservative

5% Proclin300

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-tag, Ala279~Ser390 (NP_035707.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

<1.0EU per 1μg (determined by the LAL method)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Secreted,Extracellular space,Extracellular matrix

Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. Mice lacking a functional copy of this gene develop severe multifocal inflammatory disease, yolk sac defects and colon cancer. [provided by RefSeq, Aug 2016]

Database

Gene ID: 21803 Tgfb1
UniProt: P04202 Tgfb1

Research Area

DATA IMAGES

GTX04319-pro WB Image

WB analysis of GTX04319-pro Mouse TGF beta 1 protein, His tag (active) using Mouse TGFb1 antibody.

GTX04319-pro Image

SDS-PAGE of GTX04319-pro Mouse TGF beta 1 protein, His tag (active).

GTX04319-pro Neutralizing/Inhibition Image

Cell viability was assessed by CCK-8 assay after incubation with recombinant GTX04319-pro Mouse TGF beta 1 protein, His tag (active) for 48h.
ED50 : 7.1μg/mL

GTX04319-pro Functional Assay Image

Cell proliferation effect of GTX04319-pro Mouse TGF beta 1 protein, His tag (active) 48h observed by inverted microscope.
(A) A549 cells cultured in DMEM, stimulated with 12.5μg/mL TGF-β1
(B) Unstimulated A549 cells cultured in DMEM

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Package

List Price ($)

10 μg

$ 399

Qty