NOTCH1 antibody [4C9]

Cat. No. GTX84020

Host	Mouse
Clonality	Monoclonal
Clone Name	4C9
Isotype	IgG2b
Application	WB, IHC-P
Reactivity	Human
Package	100 μl ($519)

See all NOTCH1 products

Datasheet
Document

View product citations for antibody GTX84020 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000
IHC-P	1:50-1:150

Not tested in other applications.

Calculated MW

273 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 2300-2556 of human NOTCH1

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

notch receptor 1 , AOS5 , AOVD1 , TAN1 , hN1

Cellular Localization

Cell membrane

Background

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia.

Database

Gene ID: 4851 NOTCH1
UniProt: P46531 NOTCH1

Research Area

DATA IMAGES

GTX84020 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX84020 NOTCH1 antibody [4C9].

GTX84020 IHC-P Image

IHC-P analysis of kidney tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84020 IHC-P Image

IHC-P analysis of kidney carcinoma tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84020 IHC-P Image

IHC-P analysis of pancreas tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84020 IHC-P Image

IHC-P analysis of endometrium tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84020 IHC-P Image

IHC-P analysis of liver tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84020 IHC-P Image

IHC-P analysis of breast tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84020 IHC-P Image

IHC-P analysis of human bladder tissue using GTX84020 NOTCH1 antibody [4C9].

GTX84020 WB Image

WB analysis of HEK293T cells transfected with NOTCH1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84020 NOTCH1 antibody [4C9].
Loading : 5 ug per lane

GTX84020 IHC-P Image

IHC-P analysis of pancreas carcinoma tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84020 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84020 IHC-P Image

IHC-P analysis of thyroid carcinoma tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84020 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84020 IHC-P Image

IHC-P analysis of human lymphoma tissue using GTX84020 NOTCH1 antibody [4C9].

GTX84020 IHC-P Image

IHC-P analysis of lung carcinoma tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84020 IHC-P Image

IHC-P analysis of human endometrium adenocarcinoma tissue using GTX84020 NOTCH1 antibody [4C9].

GTX84020 WB Image

WB analysis of wild type and NOTCH1 knock out HeLa cell lysate(10μg per lane) using GTX84020 NOTCH1 antibody [4C9].
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

NOTCH1 antibody [4C9]

Cat. No. GTX84020

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84020 IHC-P Image

GTX84020 IHC-P Image

GTX84020 IHC-P Image

GTX84020 IHC-P Image

GTX84020 IHC-P Image

GTX84020 IHC-P Image

GTX84020 IHC-P Image

GTX84020 IHC-P Image

GTX84020 WB Image

GTX84020 IHC-P Image

GTX84020 IHC-P Image

GTX84020 IHC-P Image

GTX84020 IHC-P Image

GTX84020 IHC-P Image

GTX84020 IHC-P Image

GTX84020 IHC-P Image

GTX84020 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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