GeneTex

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United States (US)

NP-40 (25%)

Cat. No. GTX30961

Application

 WB, ICC/IF, ELISA, IHC
Package
50 ml ($129)
See all Detergent products
PRODUCT

Summary

NP-40 (25%)

APPLICATION

Application Note

For diluting antibodies use 1:1000 in buffers (1 ml into 999 ml).
Washing buffer use 1:500 (1 ml into 499 ml).
The optimum dilution should be determined by the individual lab.

PROPERTIES

Form

Liquid

Buffer

This detergent is supplied as 25% in water.

Preservative

0.05% preservative, not contain azide or mercury preservative

Storage

Store at room temperature.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Non ionic detergents like NP40 are used in immunoassays for diluting antibodies to lower background, and non-specific antigen-antibody (ag-ab) reaction. This detergent is also used in washing buffer from 0.1- 0.5% in all immunoassays (ELISA, Immunohistochemistry (IHC), Blot assays and others) to reduce background. Tergitol-type NP-40, which is nonyl phenoxylpolyethoxylethanol, lower surface tension of water.

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Package List Price ($)
$ 129