APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
1:50-1:200 |
1:1000-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
LGNSO transfected HeLa , human ESC
Predict Reactivity
Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.66 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
From tissue culture supernatant
Conjugation
Unconjugated
RRID
AB_11176459
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4
Cellular Localization
Nucleus
Background
This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX627419 IP Image
Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX627419) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX627419) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
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GTX627419 IHC-P Image
Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using OCT3/4(GTX627419) antibody at 1:200 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX627419 WB Image
Mouse embryonic stem cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (GTX627419) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627419 IHC-P Image
Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX627419 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (GTX627419) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627419 IHC-P Image
Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human cervical carcinoma.Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX627419 WB Image
Oct4 antibody [GT486] detects Oct4 protein by western blot analysis. A. 30 μg human ESC whole cell lysate/extract 10% SDS-PAGE (GTX627419) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627419 FACS Image
Oct4 antibody [GT486] detects POU5F protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [GT486] dilution: 1:50
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GTX627419 WB Image
Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (GTX627419) diluted at 1:120000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627419 ICC/IF Image
Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.
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GTX627419 WB Image
Sample (10 μg of whole cell lysate) A: Non-transfected HeLa lysates B: HeLa transfected recombinant Oct4 protein 10% SDS PAGE GTX627419 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627419 ICC/IF Image
Oct4 antibody detects Oct4 protein by immunofluorescent analysis. Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min. Green: Oct4 protein stained by Oct4 antibody (GTX627419) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
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GTX627419 WB Image
The data was published in the 2020 in Sci Rep. PMID: 32398775
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GTX627419 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 31878324
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REFERENCE
REVIEW
Oct4 antibody [GT486]
Cat. No. GTX627419
Rating
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( Average 4.3 based on 3 users reviews)
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Application
Western Blot(WB)
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( Average 4.5 based on 2 users reviews)
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Flow cytometry(FACS)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 30-May-2024
Application Tested :
WB
Sample Species :
Hu
Sample :
human iPSC cells
Amount used :
6μg
Blocking :
5% milk, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 17-Jun-2016
Application Tested :
WB
Sample Species :
Hu
Sample :
HeLa cells
Amount used :
60μg
Blocking :
5% milk, 24°C, 1.5Hr
Primary Antibody :
1:1200, 4°C, 16Hr
Date :
Anonymous submitted on 16-May-2013
Application Tested :
FACS
Sample Species :
Hu
Sample :
hES cells
Blocking :
2% serum, 4°C, 0.5Hr
Fixation :
70% ethanol
Primary Antibody :
1:50, 4°C, 0.5Hr