PRODUCT
Summary
PSD95 antibody recognizes postsynaptic density protein 95, an ~80 kDa multidomain scaffold protein enriched at neuron postsynaptic excitatory membranes. It belongs to the membrane-associated guanylate kinase (MAGUK) family, which also contains PSD-93, SAP97 and SAP102. PSD95 is one of the most abundant proteins in the PSD, and functions in synapse maturation, strength, and plasticity.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse brain , mouse fetal brain , rat brain , mouse eye
Predict Reactivity
Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.39 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse PSD95. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886821
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
discs large MAGUK scaffold protein 4 , Dlgh4 , PSD-95 , PSD95 , SAP90 , SAP90A
Cellular Localization
Membrane , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density , synapse
Background
Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
Database
Research Area
DATA IMAGES
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GTX133091 IHC-Fr Image
PSD95 antibody detects PSD95 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: PSD95 protein stained by PSD95 antibody (GTX133091) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX133091 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PSD95 antibody (GTX133091) diluted at 1:1000.
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GTX133091 IHC-P Image
PSD95 antibody detects PSD95 protein expression by immunohistochemical analysis.
Sample: Paraffin-Embedded adult mouse retina.
Green: PSD95 protein stained by PSD95 antibody (GTX133091) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133091 ICC/IF Image
PSD95 antibody detects PSD95 protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PSD95 protein stained by PSD95 antibody (GTX133091) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX133091 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PSD95 antibody (GTX133091) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133091 IHC-P Image
PSD95 antibody detects PSD95 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: PSD95 stained by PSD95 antibody (GTX133091) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1 , a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133091 WB Image
The data was published in the 2022 in Front Aging Neurosci. PMID: 35264943
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REFERENCE
REVIEW
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