Date :
Anonymous submitted on 22-Jun-2018
Application Tested :
ICC/IF
Sample Species :
PCV2
Sample :
PK-15 cells
Blocking :
1% BSA in PBS with 0.05% Tween, 37°C, 1Hr
Fixation :
4% Paraformaldehyde in PBS, pH7.4, 25°C, 0.3Hr
Permeabilization /Antigen retrieval :
0.2% TritonX-100 in PBS, 25°C, 40 minHr
Primary Antibody :
1:50, 37°C, 1Hr
Figure Note :
PCV2 non-infected (upper left) and infected (upper right, lower left and lower right) PK-15 cells. Image courtesy of Dr. Karuppannan from the lab "Opriessnig Laboratory, Dept of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA".