GeneTex

  • Country / Location Selection

United States (US)

Vasa antibody

Anti-Vasa antibody used in IHC (Whole mount) (IHC-Wm). GTX128306
Anti-Vasa antibody used in Western Blot (WB). GTX128306
Anti-Vasa antibody used in IHC (Whole mount) (IHC-Wm). GTX128306
Anti-Vasa antibody used in Western Blot (WB). GTX128306
Anti-Vasa antibody used in Western Blot (WB). GTX128306
Anti-Vasa antibody used in IHC (Paraffin sections) (IHC-P). GTX128306

Cat. No. GTX128306

Reference 18

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm, IHC

Reactivity

 Zebrafish, Japanese Medaka, Fish
Package
100 μl ($399),
25 μl ($169)
See all Vasa products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Wm 1:100-1:500
IHC Assay dependent
Not tested in other applications.

Positive Control

Zebrafish 10 hpf

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish Vasa. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2847856

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 , vas , vasa , vlg , wu:fi24g05 , zgc:109812 , zgc:158535

Database

Research Area

DATA IMAGES

Anti-Vasa antibody used in IHC (Whole mount) (IHC-Wm). GTX128306

GTX128306 IHC-Wm Image

vasa antibody detects vasa protein at primordial germ cell on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
vasa antibody (GTX128306) dilution: 1:200.
Anti-Vasa antibody used in Western Blot (WB). GTX128306

GTX128306 WB Image

Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Vasa antibody (GTX128306) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vasa antibody used in IHC (Whole mount) (IHC-Wm). GTX128306

GTX128306 IHC-Wm Image

Vasa antibody detects Vasa protein on Medaka by whole mount immunohistochemical analysis.
Sample: 7 days-post-fertilization medaka embryo.
Vasa antibody (GTX128306) dilution: 1:100.
Anti-Vasa antibody used in Western Blot (WB). GTX128306

GTX128306 WB Image

Vasa antibody detects Vasa protein by western blot analysis.
A. 30 μg Zebrafish 10 hpf lysate/extract
7.5% SDS-PAGE
Vasa antibody (GTX128306) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vasa antibody used in Western Blot (WB). GTX128306

GTX128306 WB Image

The data was published in the journal PLoS Genet in 2018.PMID: 30399145

Anti-Vasa antibody used in IHC (Paraffin sections) (IHC-P). GTX128306

GTX128306 IHC-P Image

The data was published in the journal Sci Rep in 2017.PMID: 28211534

REFERENCE

Application Reference

Westerich KJ et al. bioRxiv 2023; Spatial organization and function of RNA molecules within phase-separated condensates are controlled by Dnd1.
Xiaoyun Sun et al. Int J Mol Sci 2022; 23 (11) Isolation and Characterization of Germline Stem Cells in Protogynous Hermaphroditic Monopterus albus.
Reactivity : Zebrafish
Gang Zhai et al. Elife 2022; 11 Augmentation of progestin signaling rescues testis organization and spermatogenesis in zebrafish with the depletion of androgen signaling.
Application : IHC-P
Reactivity : Zebrafish
Stefan Redl et al. Development 2021; 148 (2) Extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Raquel Atienzar-Aroca et al. Biology (Basel) 2021; 10 (2) Knockout of myoc Provides Evidence for the Role of Myocilin in Zebrafish Sex Determination Associated with Wnt Signalling Downregulation.
Application : IHC-Wm
Reactivity : Zebrafish
Show More +
Submit a Reference

REVIEW

There are currently no reviews for Vasa antibody (GTX128306). Be the first to share your experience with this product.
Submit a Review
Application Reference
Westerich KJ et al. bioRxiv 2023; Spatial organization and function of RNA molecules within phase-separated condensates are controlled by Dnd1.
Xiaoyun Sun et al. Int J Mol Sci 2022; 23 (11) Isolation and Characterization of Germline Stem Cells in Protogynous Hermaphroditic Monopterus albus.
Reactivity : Zebrafish
Gang Zhai et al. Elife 2022; 11 Augmentation of progestin signaling rescues testis organization and spermatogenesis in zebrafish with the depletion of androgen signaling.
Application : IHC-P
Reactivity : Zebrafish
Stefan Redl et al. Development 2021; 148 (2) Extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Raquel Atienzar-Aroca et al. Biology (Basel) 2021; 10 (2) Knockout of myoc Provides Evidence for the Role of Myocilin in Zebrafish Sex Determination Associated with Wnt Signalling Downregulation.
Application : IHC-Wm
Reactivity : Zebrafish
Hansen C.L. et al. Genesis. 2021; 59 (10):e23452 Conserved germ plasm characteristics across the Danio and Devario lineages.
Application : ICC/IF
Reactivity : Zebrafish
Higaki S et al. J Fish Biol 2021; (Epub) Germ cell-specific expression of Venus by Tol2-mediated transgenesis in endangered endemic cyprinid Honmoroko (Gnathopogon caerulescens).
Lai F et al. Int J Biol Sci 2021; 17 (8):2009-2020 Identification of Histone Modifications Reveals a Role of H2b Monoubiquitination in Transcriptional Regulation of dmrt1 in Monopterus albus.
Reactivity : Fish
Dedukh D et al. Sci Rep 2020; 10 (1):8720 Micronuclei in germ cells of hybrid frogs from Pelophylax esculentus complex contain gradually eliminated chromosomes.
Application : IHC-Wm
Ye D et al. Mar Biotechnol (NY) 2019; 21 (2):217-228 Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealed?by Lifetime Labeling of Germline.
Yan Zhao et al. Aquaculture 2019; 515:734535 Gonad development examination of high-temperature–treated genetically female Nile tilapia.
Application : IHC-P
Reactivity : Fish
Zhang X et al. PLoS One 2019; 14 (8):e0220364 Transcriptomic profile of early zebrafish PGCs by single cell sequencing.
Application : IHC-Wm
Krishnakumar P et al. PLoS Genet 2018; 14 (11):e1007696 Functional equivalence of germ plasm organizers.
Application : WB
Reactivity : Zebrafish
Higaki S et al. Fish Physiol Biochem 2017; (Epub) Cryopreservation of male and female gonial cells by vitrification in the critically endangered cyprinid honmoroko Gnathopogon caerulescens.
Higaki S et al. Zygote 2017;:43110 Successful vitrification of whole juvenile testis in the critically endangered cyprinid honmoroko (Gnathopogon caerulescens).
Application : IHC-P
Reactivity : Zebrafish
Liu Y et al. Biochimie 2017; (Epub) Nanog suppresses the expression of vasa by directly regulating nlk1 in the early zebrafish embryo.
Application : WB
Reactivity : Zebrafish
Higaki S et al. Sci Rep 2017; 7:42852 In vitro differentiation of fertile sperm from cryopreserved spermatogonia of the endangered endemic cyprinid honmoroko (Gnathopogon caerulescens).
Application : IHC-P
Reactivity : Zebrafish
Shen J et al. Biochem Biophys Res Commun 2016; 478 (2):858-63 Diethylnitrosamine-induced expression of germline-specific genes and pluripotency factors, including vasa and oct4, in medaka somatic cells.
Application : IHC
Reactivity : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169