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YAP1 antibody

KO/KD Comparison Overexpression
Anti-YAP1 antibody used in Western Blot (WB). GTX129151
Anti-YAP1 antibody used in Western Blot (WB). GTX129151
Anti-YAP1 antibody used in Western Blot (WB). GTX129151
Anti-YAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129151
Anti-YAP1 antibody used in Western Blot (WB). GTX129151
Anti-YAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129151
Anti-YAP1 antibody used in Immunoprecipitation (IP). GTX129151
Anti-YAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129151
Anti-YAP1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129151
Anti-YAP1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129151

Cat. No. GTX129151

Reference 13

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

 Human, Mouse, Deer
Package
100 μl ($399),
25 μl ($169)
See all YAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HeLa , YAP1-transfected 293T

Predict Reactivity

Rat, Zebrafish, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human YAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885910

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Yes associated protein 1 , COB1 , YAP , YAP2 , YAP65 , YKI

Background

This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-YAP1 antibody used in Western Blot (WB). GTX129151

GTX129151 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody (GTX129151) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-YAP1 antibody used in Western Blot (WB). GTX129151

GTX129151 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with YAP1 antibody (GTX129151) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-YAP1 antibody used in Western Blot (WB). GTX129151

GTX129151 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody (GTX129151) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-YAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129151

GTX129151 ICC/IF Image

YAP1 antibody detects YAP1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: YAP1 protein stained by YAP1 antibody (GTX129151) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-YAP1 antibody used in Western Blot (WB). GTX129151

GTX129151 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody (GTX129151) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-YAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129151

GTX129151 IHC-P Image

YAP1 antibody detects YAP1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human glioblastoma.
YAP1 stained by YAP1 antibody (GTX129151) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-YAP1 antibody used in Immunoprecipitation (IP). GTX129151

GTX129151 IP Image

YAP1 antibody immunoprecipitates YAP1 protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of YAP1 protein by 4 μg YAP1 antibody (GTX129151)
7.5 % SDS-PAGE
The immunoprecipitated YAP1 protein was detected by YAP1 antibody (GTX129151) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-YAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129151

GTX129151 IHC-P Image

YAP1 antibody detects YAP1 protein at nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
YAP1 antibody (GTX129151) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-YAP1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129151

GTX129151 IHC-Fr Image

Overexpression

The data was published in the journal Nat Commun in 2019. PMID: 31477715

Anti-YAP1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129151

GTX129151 IHC-Fr Image

The data was published in the journal Nat Commun in 2019. PMID: 31477715

REFERENCE

Application Reference

Blokland KEC et al. Front Pharmacol 2022; 13 : 989169 Substrate stiffness engineered to replicate disease conditions influence senescence and fibrotic responses in primary lung fibroblasts.
Application : ICC/IF
Reactivity : Human
Xuyang Sun et al. Int J Mol Sci 2022; 23 (9) PRDX2 Knockdown Inhibits Extracellular Matrix Synthesis of Chondrocytes by Inhibiting Wnt5a/YAP1/CTGF and Activating IL-6/JAK2/STAT3 Pathways in Deer Antler.
Application : WB
Xuyang Sun et al. Int J Mol Sci 2022; 23 (2) Melatonin Promotes Antler Growth by Accelerating MT1-Mediated Mesenchymal Cell Differentiation and Inhibiting VEGF-Induced Degeneration of Chondrocytes.
Application : WB
Reactivity : Deer
Huang PY et al. Am J Cancer Res 2022; 12 (2) : 873-892 A novel HDAC11 inhibitor potentiates the tumoricidal effects of cordycepin against malignant peripheral nerve sheath tumor through the Hippo signaling pathway.
Application : WB
Reactivity : Human
Sun X et al. Int. J. Mol. Sci. 2022; 23 (2) : 759 Melatonin Promotes Antler Growth by Accelerating MT1-Mediated Mesenchymal Cell Differentiation and Inhibiting VEGF-Induced Degeneration of Chondrocytes.
Application : WB
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REVIEW

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Application Reference
Blokland KEC et al. Front Pharmacol 2022; 13:989169 Substrate stiffness engineered to replicate disease conditions influence senescence and fibrotic responses in primary lung fibroblasts.
Application : ICC/IF
Reactivity : Human
Xuyang Sun et al. Int J Mol Sci 2022; 23 (9) PRDX2 Knockdown Inhibits Extracellular Matrix Synthesis of Chondrocytes by Inhibiting Wnt5a/YAP1/CTGF and Activating IL-6/JAK2/STAT3 Pathways in Deer Antler.
Application : WB
Xuyang Sun et al. Int J Mol Sci 2022; 23 (2) Melatonin Promotes Antler Growth by Accelerating MT1-Mediated Mesenchymal Cell Differentiation and Inhibiting VEGF-Induced Degeneration of Chondrocytes.
Application : WB
Reactivity : Deer
Huang PY et al. Am J Cancer Res 2022; 12 (2):873-892 A novel HDAC11 inhibitor potentiates the tumoricidal effects of cordycepin against malignant peripheral nerve sheath tumor through the Hippo signaling pathway.
Application : WB
Reactivity : Human
Sun X et al. Int. J. Mol. Sci. 2022; 23 (2):759 Melatonin Promotes Antler Growth by Accelerating MT1-Mediated Mesenchymal Cell Differentiation and Inhibiting VEGF-Induced Degeneration of Chondrocytes.
Application : WB
Tanaka H et al. Commun Biol 2021; 4 (1):1175 HMGB1 signaling phosphorylates Ku70 and impairs DNA damage repair in Alzheimer's disease pathology.
Application : IHC-P
Reactivity : Human
Zhu Q et al. Oncol Lett 2020; 19 (1):103-112 Linc-OIP5 loss regulates migration and invasion in MDA-MB-231 breast cancer cells by inhibiting YAP1/JAG1 signaling.
Li CL et al. Anticancer Res 2020; 40 (10):5529-5538 Curcumol Suppresses Triple-negative Breast Cancer Metastasis by Attenuating Anoikis Resistance via Inhibition of Skp2-mediated Transcriptional Addiction.
Application : WB
Reactivity : Human
Zhu Q et al. Biol Res 2020; 53 (1):5 Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor microenvironment.
Application : WB
Reactivity : Human
Miesje van der Stoel et al. bioRxiv 2019; (Epub) DLC1 is a direct target of activated YAP/TAZ that drives collective migration and sprouting angiogenesis.
Application : WB
Reactivity : Human
Pajtler KW et al. Nat Commun 2019; 10 (1):3914 YAP1 subgroup supratentorial ependymoma requires TEAD and nuclear factor I-mediated transcriptional programmes for tumorigenesis.
Application : WB
Reactivity : Human
Chen MJ et al. Pathol Res Pract 2019; (Epub) Association of nuclear localization of SHP2 and YAP1 with unfavorable prognosis in non-small cell lung cancer.
Application : IHC-P
Reactivity : Human
Lin YC et al. Clin Otolaryngol 2016; (Epub) Immunohistochemical studies and fluorodeoxyglucose uptake on positron emission tomography in pharyngeal cancer for predicting radiotherapy-based treatment outcomes.
Application : IHC-P
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169