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Yellow Fever virus NS3 protein antibody

Anti-Yellow Fever virus NS3 protein antibody used in Western Blot (WB). GTX133959

Cat. No. GTX133959

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Yellow Fever virus
Package
100 μl ($399),
25 μl ($169)
See all Yellow Fever virus NS3 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Positive Control

YFV NS3-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Yellow Fever virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Yellow Fever Virus NS3 protein (Yellow fever virus (strain 17D vaccine)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887155

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Yellow Fever Virus Non-structural protein 3 , Yellow Fever Virus NS3 , YFV Non-structural protein 3 , YFV NS3

Research Area

DATA IMAGES

Anti-Yellow Fever virus NS3 protein antibody used in Western Blot (WB). GTX133959

GTX133959 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with YFV virus NS3 protein antibody (GTX133959) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Gao Z et al. Antiviral Res 2020; 182 : 104907 Development of antibody-based assays for high throughput discovery and mechanistic study of antiviral agents against yellow fever virus.
Application : WB, ICC/IF
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169