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Zika virus NS2B protein antibody

Orthogonal
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308
Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133308
Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133308
Anti-Zika virus NS2B protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX133308
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308

Cat. No. GTX133308

Reference 35
8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-P (cell pellet)

Reactivity

 Zika virus
Package
100 μl ($399),
25 μl ($169)
See all Zika virus NS2B protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zika virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Zika virus NS2B protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2715494

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zika virus Non-structural protein 2B , Zika virus NS2B , ZIKV Non-structural protein 2B , ZIKV NS2B

Research Area

DATA IMAGES

Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308

GTX133308 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Zika virus NS2B protein antibody (GTX133308) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133308

GTX133308 ICC/IF Image

Immunofluorescent analysis ofZika Virus-PRVABC59 infected (A) and non-infected (B) vero cells using Zika virus NS2B protein antibody (GTX133308).
Green: Zika virus NS2B protein antibody (GTX133308) diluted at 1:4000.
This image was provided courtesy of cooperative research laboratories.

Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133308

GTX133308 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus NS2B protein antibody (GTX133308).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus NS2B protein antibody (GTX133308) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Zika virus NS2B protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX133308

GTX133308 IHC-P (cell pellet) Image

Zika virus NS2B protein antibody detects Zika virus NS2B protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded zika virus C6/36.
Green: Zika virus NS2B protein stained by Zika virus NS2B protein antibody (GTX133308) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308

GTX133308 WB Image

Orthogonal

The data was published in the journal Elife in 2018.PMID: 30511641

Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308

GTX133308 WB Image

The data was published in the journal PLoS Negl Trop Dis in 2017.PMID: 28481898

REFERENCE

Application Reference

Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
Manet C et al. J Neuroinflammation 2022; 19 (1) : 307 Zika virus infection of mature neurons from immunocompetent mice generates a disease-associated microglia and a tauopathy-like phenotype in link with a delayed interferon beta response.
Application : ICC/IF
Zhuang Wang et al. Viruses 2022; 14 (6) Azelnidipine Exhibits In Vitro and In Vivo Antiviral Effects against Flavivirus Infections by Targeting the Viral RdRp.
Kung YA et al. mBio 2022; : e0271721 Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis.
Application : ICC/IF
Jose Carlos Ponce-Rojas et al. J Clin Microbiol 2021; 59 (4) A Fast and Accessible Method for the Isolation of RNA, DNA, and Protein To Facilitate the Detection of SARS-CoV-2.
Application : WB
Show More +
Submit a Reference

REVIEW

Zika virus NS2B protein antibody Cat. No. GTX133308

Rating
( Average 5 based on 8 users reviews)
Application
Western Blot(WB)
( Average 5 based on 5 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 2 users reviews)
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308
Date : Anonymous submitted on 25-May-2018
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected Vero cells
Amount used : 20 μg
Blocking : 5% non-fat milk, 22°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : The researcher provided "Cpd-E2 is a novel antiviral affecting Zika polyprotein translation".
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308
Date : Anonymous submitted on 28-Nov-2017
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika infected Huh-7 cells
Amount used : 10 μg
Blocking : 5% milk
Primary Antibody : 1:2000, 4°C, 16Hr
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308
Date : Anonymous submitted on 28-Nov-2017
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika infected C6/36 cells, Zika infected vero cells
Amount used : 10 μg
Blocking : 5% milk
Primary Antibody : 1:2000, 4°C, 16Hr
Anti-Zika virus NS2B protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133308
Date : Anonymous submitted on 08-Sep-2016
Score :
Application Tested : IHC-P
Sample Species : ZIKV
Sample : Zika-infected mouse spleen tissues
Primary Antibody : 1:100, 4°C, 16Hr
Figure Note : A: mouse red pulp tissue, B: Zika virus infected mouse red pulp tissue
Anti-Zika virus NS2B protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133308
Date : Anonymous submitted on 08-Sep-2016
Score :
Application Tested : IHC-P
Sample Species : ZIKV
Sample : Zika-infected mouse spleen tissues
Primary Antibody : 1:100, 4°C, 16Hr
Figure Note : A: mouse white pulp tissue, B: Zika virus infected mouse white pulp tissue
Anti-Zika virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133308
Date : Anonymous submitted on 14-Jun-2016
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Fixation : methanol + acetone
Primary Antibody : 1:4000, 4°C, 16Hr
Figure Note : A: Zika Virus-PRVABC59 infected vero cells. (MOI=1 24h), B: vero cell
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308
Date : Anonymous submitted on 04-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected C6/36 cells
Amount used : 20μg
Blocking : 5% non-fat milk
Primary Antibody : 1:4000, 4°C, 16Hr
Lane Description : Lane 1: C6/36 cells, Lane 2: Zika Virus-PRVABC59 infected C6/36 cells
Anti-Zika virus NS2B protein antibody used in Western Blot (WB). GTX133308
Date : Anonymous submitted on 02-Jun-2016
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : Zika Virus-PRVABC59 infected vero cells
Amount used : 15μg
Blocking : 5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vero cells, Lane 2: Zika Virus-PRVABC59 infected vero cells. (MOI=0.1 96h)
Show More +
Submit a Review
Application Reference
Y Lan et al. Nat Commun 2023; Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles
Application : WB
Manet C et al. J Neuroinflammation 2022; 19 (1):307 Zika virus infection of mature neurons from immunocompetent mice generates a disease-associated microglia and a tauopathy-like phenotype in link with a delayed interferon beta response.
Application : ICC/IF
Zhuang Wang et al. Viruses 2022; 14 (6) Azelnidipine Exhibits In Vitro and In Vivo Antiviral Effects against Flavivirus Infections by Targeting the Viral RdRp.
Kung YA et al. mBio 2022;:e0271721 Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis.
Application : ICC/IF
Jose Carlos Ponce-Rojas et al. J Clin Microbiol 2021; 59 (4) A Fast and Accessible Method for the Isolation of RNA, DNA, and Protein To Facilitate the Detection of SARS-CoV-2.
Application : WB
Ferreira RO et al. Viruses 2021; 13 (10) Effect of Serial Systemic and Intratumoral Injections of Oncolytic ZIKVBR in Mice Bearing Embryonal CNS Tumors.
Application : IHC-Fr
Gavino-Leopoldino D et al. J Virol 2021; 95 (22):e0090421 Skeletal Muscle Is an Early Site of Zika Virus Replication and Injury, Which Impairs Myogenesis.
Application : ICC/IF
Shelton SM et al. eNeuro 2021; 8 (5) Forebrain Neural Precursor Cells Are Differentially Vulnerable to Zika Virus Infection.
Application : IHC-Fr
Matz K et al. Microorganisms 2021; 9 (6) Favipiravir (T-705) Protects IFNAR-/- Mice against Lethal Zika Virus Infection in a Sex-Dependent Manner.
Application : IHC-P
Pletnev AG et al. Nat Commun 2021; 12 (1):2469 Epididymal epithelium propels early sexual transmission of Zika virus in the absence of interferon signaling.
Silva BVM et al. Biosensors (Basel) 2021; 11 (5) A Label and Probe-Free Zika Virus Immunosensor Prussian Blue@carbon Nanotube-Based for Amperometric Detection of the NS2B Protein.
Shi H et al. Biotechnol Bioeng 2021; (Epub) Kinetics of Asian and African Zika Virus Lineages over Single-cycle and Multi-cycle Growth in Culture: gene expression, cell killing, virus production, and mathematical modeling.
Rory K M Long et al. Sci Rep 2020; 10 (1):20937 Super resolution microscopy and deep learning identify Zika virus reorganization of the endoplasmic reticulum.
Application : ICC/IF
Kaid C et al. Mol Ther 2020; 28 (5):1276-1286 Safety, Tumor Reduction, and Clinical Impact of Zika Virus Injection in Dogs with Advanced-Stage Brain Tumors.
Xiangling Cui et al. Front Pharmacol. 2020; 11:514313 Identification of Theaflavin-3,3'-Digallate as a Novel Zika Virus Protease Inhibitor.
Application : WB
Chen J et al. mBio 2020; 11 (5) Placental Alkaline Phosphatase Promotes Zika Virus Replication by Stabilizing Viral Proteins through BIP.
Application : ICC/IF
Tsetsarkin KA et al. PLoS Pathog 2020; 16 (7):e1008601 Zika virus tropism during early infection?of the?testicular interstitium and its role in viral pathogenesis in the testes.
Application : IHC-P
Wu Y et al. PLoS Negl Trop Dis 2020; 14 (3):e0008083 C19orf66 interrupts Zika virus replication by inducing lysosomal degradation of viral NS3.
Application : WB
Zhang X et al. MBio 2019; 10 (5) Zika Virus NS2A-Mediated Virion Assembly.
Application : WB
Figueiredo CP et al. Nat Commun 2019; 10 (1):3890 Zika virus replicates in adult human brain tissue and impairs synapses and memory in mice.
Application : IHC-P, IHC-Fr
Barrows NJ et al. Sci Rep 2019; 9 (1):9711 Dual roles for the ER membrane protein complex in flavivirus infection: viral entry and protein biogenesis.
Application : WB
Branche E et al. Sci Rep 2019; 9 (1):9857 Human Polyclonal Antibodies Prevent Lethal Zika Virus Infection in Mice.
Application : ICC/IF, IHC-P
Regla-Nava JA et al. PLoS Negl Trop Dis 2019; 13 (2):e0007080 Detection of Zika virus in mouse mammary gland and breast milk.
Application : ICC/IF, IHC-P
Carlin AF et al. Proc Natl Acad Sci U S A 2018; 115 (39):E9172-E9181 Deconvolution of pro- and antiviral genomic responses in Zika virus-infected and bystander macrophages.
Tsetsarkin KA et al. Nat Commun 2018; 9 (1):5350 Routes of Zika virus dissemination in the testis and epididymis of immunodeficient mice.
Soto-Acosta R et al. Elife 2018; 7 Fragile X mental retardation protein is a Zika virus restriction factor that is antagonized by subgenomic flaviviral RNA.
Application : WB
Wu YH et al. PLoS Negl Trop Dis 2018; 12 (10):e0006848 ICR suckling mouse model of Zika virus infection for disease modeling and drug validation.
Application : WB
Chen J et al. Nat Microbiol 2018; 3 (3):302-309 axl promotes zika virus infection in astrocytes by antagonizing type i * signalling.
Application : WB
Fernandez MP et al. JAMA Ophthalmol 2017; 135 (11):1163-1169 Ocular Histopathologic Features of Congenital Zika Syndrome.
Boigard H et al. PLoS Negl Trop Dis 2017; 11 (5):e0005608 Zika virus-like particle (VLP) based vaccine.
Fernandez M.P. et al. JAMA Ophthalmology 2017; (Epub) Ocular Histopathologic Features of Congenital Zika Syndrome.
Application : IHC-P
Bollweg BC et al. The Journal of Pathology 2017; (Epub) Optimization of Commercially Available Zika Virus Antibodies for Use in a Laboratory Developed Immunohistochemical Assay.
Application : IHC-P
Nguyen SM et al. PLoS Pathog 2017; 13 (5):e1006378 Highly efficient maternal-fetal Zika virus transmission in pregnant rhesus macaques.
Application : IHC-P
Boigard H et al. PLoS Negl Trop Dis 2017; (Epub) Zika virus-like particle (VLP) based vaccine.
Application : WB
Tang WW et al. Cell Reports 2016; (Epub) A Mouse Model of Zika Virus Sexual Transmission and Vaginal Viral Replication.
Application : IHC-P
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169