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c-Myc antibody

KO/KD Orthogonal Overexpression
Anti-c-Myc antibody used in ChIP assay (ChIP assay). GTX103436
Anti-c-Myc antibody used in Immunoprecipitation (IP). GTX103436
Anti-c-Myc antibody used in Western Blot (WB). GTX103436
Anti-c-Myc antibody used in Western Blot (WB). GTX103436
Anti-c-Myc antibody used in Western Blot (WB). GTX103436
Anti-c-Myc antibody used in Western Blot (WB). GTX103436
Anti-c-Myc antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103436
Anti-c-Myc antibody used in Western Blot (WB). GTX103436
Anti-c-Myc antibody used in IHC (Frozen sections) (IHC-Fr). GTX103436
Anti-c-Myc antibody used in Western Blot (WB). GTX103436
Anti-c-Myc antibody used in Western Blot (WB). GTX103436
Anti-c-Myc antibody used in ChIP assay (ChIP assay). GTX103436

Cat. No. GTX103436

Reference 29

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay

Reactivity

 Human, Mouse, Xenopus laevis
Package
100 μl ($399),
25 μl ($169)
See all c-Myc products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr Assay dependent
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

293T

Product Note

This antibody can detect the endogenous c-Myc, but it is unable to recognize the c-Myc tag.

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Cat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human c-Myc. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162914

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MYC proto-oncogene, bHLH transcription factor , MRTL , MYCC , bHLHe39 , c-Myc

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-c-Myc antibody used in ChIP assay (ChIP assay). GTX103436

GTX103436 ChIP assay Image

c-Myc antibody immunoprecipitates c-Myc protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of c-Myc antibody (GTX103436) The precipitated DNA was detected by PCR with primer set targeting to mSHMT promoter.

Anti-c-Myc antibody used in Immunoprecipitation (IP). GTX103436

GTX103436 IP Image

c-Myc antibody immunoprecipitates c-Myc protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 20 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of c-Myc protein by 2.5 μg of c-Myc antibody (GTX103436) 10% SDS-PAGE The immunoprecipitated c-Myc protein was detected by c-Myc antibody (GTX103436) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-c-Myc antibody used in Western Blot (WB). GTX103436

GTX103436 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc antibody (GTX103436) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Myc antibody used in Western Blot (WB). GTX103436

GTX103436 WB Image

KO/KD

Wild-type (WT) and MYC knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc antibody (GTX103436) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Myc antibody used in Western Blot (WB). GTX103436

GTX103436 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc antibody (GTX103436) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Myc antibody used in Western Blot (WB). GTX103436

GTX103436 WB Image

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
10% SDS PAGE
GTX103436 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Myc antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103436

GTX103436 ICC/IF Image

Orthogonal

The data was published in the journal Oncogenesis in 2020.PMID: 32350243

Anti-c-Myc antibody used in Western Blot (WB). GTX103436

GTX103436 WB Image

Overexpression

The data was published in the journal Sci Rep in 2018.PMID: 29875371

Anti-c-Myc antibody used in IHC (Frozen sections) (IHC-Fr). GTX103436

GTX103436 IHC-Fr Image

Orthogonal

The data was published in the journal Front Mol Neurosci in 2017.PMID: 29234272

Anti-c-Myc antibody used in Western Blot (WB). GTX103436

GTX103436 WB Image

Orthogonal

The data was published in the journal Front Mol Neurosci in 2017.PMID: 29234272

Anti-c-Myc antibody used in Western Blot (WB). GTX103436

GTX103436 WB Image

The data was published in the journal PLoS One in 2015. PMID: 26226629

Anti-c-Myc antibody used in ChIP assay (ChIP assay). GTX103436

GTX103436 ChIP assay Image

The data was published in the journal Pharmacol Res Perspect in 2018. PMID: 30455960

REFERENCE

Application Reference

Su WC et al. In Vivo 2023; 37 (6) : 2524-2532 Thiamet G as a Potential Treatment for Polycystic Kidney Disease.
Lin CY et al. Cell Transplant 2023; 32 : 9.6369E+15 Coating-Free Culture Medium for Establishing and Maintaining Human Induced Pluripotent Stem Cells.
Mushtaq I et al. Front Oncol 2022; 12 : 919351 Telomere Attrition With Concomitant hTERT Overexpression Involved in the Progression of Gastric Cancer May Have Prognostic and Clinical Implications in High-Risk Population Group From North India.
Application : IHC-P
Reactivity : Human
Huang CY et al. Life (Basel) 2021; 11 (9) Calcitriol Suppresses Warburg Effect and Cell Growth in Human Colorectal Cancer Cells.
Application : WB
Reactivity : Human
Hsu WJ et al. J Pers Med 2021; 11 (10) PRMT1 Confers Resistance to Olaparib via Modulating MYC Signaling in Triple-Negative Breast Cancer.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Su WC et al. In Vivo 2023; 37 (6):2524-2532 Thiamet G as a Potential Treatment for Polycystic Kidney Disease.
Lin CY et al. Cell Transplant 2023; 32:9.6369E+15 Coating-Free Culture Medium for Establishing and Maintaining Human Induced Pluripotent Stem Cells.
Mushtaq I et al. Front Oncol 2022; 12:919351 Telomere Attrition With Concomitant hTERT Overexpression Involved in the Progression of Gastric Cancer May Have Prognostic and Clinical Implications in High-Risk Population Group From North India.
Application : IHC-P
Reactivity : Human
Huang CY et al. Life (Basel) 2021; 11 (9) Calcitriol Suppresses Warburg Effect and Cell Growth in Human Colorectal Cancer Cells.
Application : WB
Reactivity : Human
Hsu WJ et al. J Pers Med 2021; 11 (10) PRMT1 Confers Resistance to Olaparib via Modulating MYC Signaling in Triple-Negative Breast Cancer.
Application : WB
Reactivity : Human
Wu MJ et al. Theranostics 2021; 11 (16):7779-7796 Targeting KDM4B that coactivates c-Myc-regulated metabolism to suppress tumor growth in castration-resistant prostate cancer.
Application : IHC-P
Qiu S et al. Front Microbiol 2021; 12:690988 Downregulation of Light-Harvesting Complex II Induces ROS-Mediated Defense Against Turnip Mosaic Virus Infection in Nicotiana benthamiana.
Application : WB
Lee CC et al. Life Sci 2021; 277:119438 A regulatory loop among CD276, miR-29c-3p, and Myc exists in cancer cells against natural killer cell cytotoxicity.
Application : WB
Colozza G et al. MicroPubl Biol 2021; 2021 Purified Bighead protein efficiently promotes head development in the South African clawed frog, Xenopus laevis.
Reactivity : Xenopus laevis
Ge J et al. J Exp Clin Cancer Res 2021; 40 (1):59 USP16 regulates castration-resistant prostate cancer cell proliferation by deubiquitinating and stablizing c-Myc.
Application : WB
Reactivity : Human
Chen J et al. Oncogenesis 2020; 9 (4):42 ACSL4 promotes hepatocellular carcinoma progression via c-Myc stability mediated by ERK/FBW7/c-Myc axis.
Reactivity : Human
Classen J et al. Sci Rep 2020; 10 (1):3181 A Munc18-1 mutant mimicking phosphorylation by Down Syndrome-related kinase Dyrk1a supports normal synaptic transmission and promotes recovery after intense activity.
Application : WB
Zhang J et al. Artif Cells Nanomed Biotechnol 2020; 48 (1):479-487 Inhibition of Wnt signalling pathway by XAV939 enhances radiosensitivity in human cervical cancer HeLa cells.
Application : WB
Reactivity : Human
Davuluri G et al. Mol Cell Biol 2019; 39 (16) Impaired Ribosomal Biogenesis by Noncanonical Degradation of β-Catenin during Hyperammonemia.
Fang Y et al. Nat Commun 2019; 10 (1):3981 CD36 inhibits 帣-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis.
Application : IHC-P
Reactivity : Human
Chen YH et al. Sci Rep 2019; 9 (1):9919 Mitochondrial Akt Signaling Modulated Reprogramming of Somatic Cells.
Reactivity : Human
Seira N et al. Pharmacol Res Perspect 2018; 6 (6):e00441 Cellular density-dependent increases in HIF-1α compete with c-Myc to down-regulate human EP4 receptor promoter activity through Sp-1-binding region.
Application : IP
Reactivity : Human
Zhang K et al. Mol Cancer 2018; 17 (1):153 Targeting histone methyltransferase G9a inhibits growth and Wnt signaling pathway by epigenetically regulating HP1α and APC2 gene expression in non-small cell lung cancer.
Application : WB
Reactivity : Human
Lin CK et al. Sci Rep 2018; 8 (1):8676 Lobohedleolide suppresses hepatitis C virus replication via JNK/c-Jun-C/EBP-mediated down-regulation of cyclooxygenase-2 expression.
Application : WB
Reactivity : Human
Chen KC et al. Journal of Food and Drug Analysis 2018; (Epub) Flavonoids Luteolin and Quercetin Inhibit RPS19 and contributes to metastasis of cancer cells through c-Myc reduction.
Application : WB
Reactivity : Human
Yang JS et al. Oncol Rep 2018; 39 (1):383-391 ITR-284 modulates cell differentiation in human chronic myelogenous leukemia K562 cells.
Ma YX et al. Front Mol Neurosci 2017; 10:391 Differential Roles of Glycogen Synthase Kinase 3 Subtypes Alpha and Beta in Cortical Development.
Application : WB
Lee YC et al. Int J Mol Sci 2017; 18 (9) Hsp90α Mediates BMI1 Expression in Breast Cancer Stem/Progenitor Cells through Facilitating Nuclear Translocation of c-Myc and EZH2.
Application : WB
Reactivity : Human
Lin HC et al. J Agric Food Chem 2017; 65 (1):51-59 Antroquinonol, a Ubiquinone Derivative from the Mushroom Antrodia camphorata, Inhibits Colon Cancer Stem Cell-like Properties: Insights into the Molecular Mechanism and Inhibitory Targets.
Application : WB
Reactivity : Human
Chung IH et al. Oncotarget 2016; 7 (16):22448-59 ChIP-on-chip analysis of thyroid hormone-regulated genes and their physiological significance.
Application : WB
Reactivity : Human
Fan F et al. Biomed Pharmacother 2016; 84:1858-1864 Nuclear PKM2 expression, an independent risk factor for ER after curative resection of hepatocellular carcinoma.
Huang CC et al. Oncotarget 2015; 6 (11):8698-708 Paxillin promotes colorectal tumor invasion and poor patient outcomes via ERK-mediated stabilization of Bcl-2 protein by phosphorylation at Serine 87.
Application : WB, IHC-P
Reactivity : Human
Wu MH et al. PLoS One 2015; 10 (7):e0134122 Macrophage Infiltration Induces Gastric Cancer Invasiveness by Activating the β-Catenin Pathway.
Fu Y et al. Med Oncol 2015; 32 (8):215 FBXW7 overexpression suppresses renal cancer cell proliferation and induces apoptosis.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169