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iNOS antibody [4E5]

Anti-iNOS antibody [4E5] used in Flow cytometry (FACS). GTX60599
Anti-iNOS antibody [4E5] used in IHC (Paraffin sections) (IHC-P). GTX60599
Anti-iNOS antibody [4E5] used in Western Blot (WB). GTX60599
Anti-iNOS antibody [4E5] used in IHC (Paraffin sections) (IHC-P). GTX60599
Anti-iNOS antibody [4E5] used in ELISA (ELISA). GTX60599

Cat. No. GTX60599

Reference 7

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4E5

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr, FACS, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all iNOS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
IHC-Fr Assay dependent
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

131 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human NOS2 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2887985

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nitric oxide synthase 2 , HEP-NOS , INOS , NOS , NOS2A

Cellular Localization

Cytoplasm, cytosol

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-iNOS antibody [4E5] used in Flow cytometry (FACS). GTX60599

GTX60599 FACS Image

FACS analysis of MCF-7 cells using GTX60599 iNOS antibody [4E5].
Green : iNOS
Red : negative control

Anti-iNOS antibody [4E5] used in IHC (Paraffin sections) (IHC-P). GTX60599

GTX60599 IHC-P Image

IHC-P analysis of breast cancer tissue using GTX60599 iNOS antibody [4E5].

Anti-iNOS antibody [4E5] used in Western Blot (WB). GTX60599

GTX60599 WB Image

WB analysis of human iNOS (AA: 997-1058) recombinant protein using GTX60599 iNOS antibody [4E5].

Anti-iNOS antibody [4E5] used in IHC (Paraffin sections) (IHC-P). GTX60599

GTX60599 IHC-P Image

IHC-P analysis of liver cancer tissue using GTX60599 iNOS antibody [4E5].

Anti-iNOS antibody [4E5] used in ELISA (ELISA). GTX60599

GTX60599 ELISA Image

ELISA analysis of antigen using GTX60599 iNOS antibody [4E5].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

REFERENCE

Application Reference

W Zhou et al. Eur J Pharmacol 2023; : 176043 Chromofungin, a chromogranin A-derived peptide, protects against sepsis-induced acute lung injury by inhibiting LBP/TLR4-dependent inflammatory signaling
Application : IHC-P
Reactivity : Mouse
Hsueh YH et al. J Orthop Translat 2023; 38 : 141-155 Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an in vitro and in vivo osteoarthritis model.
Li J et al. Ann Transl Med 2021; 9 (20) : 1534 Lupeol reduces M1 macrophage polarization to attenuate immunologic dissonance and fatty acid deposition in rats with diet-induced metabolic syndrome.
Application : FACS
Reactivity : Rat
Liang X et al. Mol Cell Biochem 2018; (Epub) Mesenchymal stem cells attenuate sepsis-induced liver injury via inhibiting * polarization of Kupffer cells.
Application : FACS
Reactivity : Human

Clone Reference

R Sadler et al. J Neurosci 2020; 40 (5):1162-1173 Short-Chain Fatty Acids Improve Poststroke Recovery via Immunological Mechanisms
Application : IHC-Fr
Reactivity : Mouse
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399